Transforming growth factor beta -3 crystals as reservoirs for slow release of active TGF- beta 3

Transforming growth factor beta -3 crystals as reservoirs for slow release of active TGF- beta 3

Transforming growth factor beta s (TGF- beta s) play critical roles in many diseased states and injury repair processes. Exogenous delivery of TGF- beta may thus have therapeutic applications. Here, crystals of TGF- beta 3 (TGF- beta 3) are being evaluated as protected reservoirs for sustained local...

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Veröffentlicht in:Journal of controlled release 2002-01, Vol.78 (1-3), p.25-34
Hauptverfasser: Jen, A, Madoerin, K, Vosbeck, K, Arvinte, T, Merkle, H P
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container_end_page 34
container_issue 1-3
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container_title Journal of controlled release
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creator Jen, A
Madoerin, K
Vosbeck, K
Arvinte, T
Merkle, H P
description Transforming growth factor beta s (TGF- beta s) play critical roles in many diseased states and injury repair processes. Exogenous delivery of TGF- beta may thus have therapeutic applications. Here, crystals of TGF- beta 3 (TGF- beta 3) are being evaluated as protected reservoirs for sustained local release. A sensitive Mv1Lu cell growth inhibition assay established that in vitro, active TGF- beta 3 can be delivered from physically stable crystals. Non-sink release experiments revealed that crystal solubility at pH 7.4 was higher in cell culture medium (2.7 plus or minus 0.1 mu g/ml) than in saline buffers ( similar to 1-1.5 mu g/ml, P
doi_str_mv 10.1016/s0168-3659(01)00490-4
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title Transforming growth factor beta -3 crystals as reservoirs for slow release of active TGF- beta 3
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