Diurnal change of cucumber [Cucumis sativus] CPD photolyase gene (CsPHR) expression and its physiological role in growth under UV-B irradiation
Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored...
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| Veröffentlicht in: | Plant and cell physiology 2002-03, Vol.43 (3), p.342-349 |
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| creator | Takahashi, S. (Tokyo Univ. (Japan)) Nakajima, N Saji, H Kondo, N |
| description | Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09:00 and 12:00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation. |
| doi_str_mv | 10.1093/pcp/pcf038 |
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(Tokyo Univ. (Japan)) ; Nakajima, N ; Saji, H ; Kondo, N</creator><creatorcontrib>Takahashi, S. (Tokyo Univ. (Japan)) ; Nakajima, N ; Saji, H ; Kondo, N</creatorcontrib><description>Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09:00 and 12:00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcf038</identifier><identifier>PMID: 11917089</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Amino Acid Sequence ; Circadian Rhythm - physiology ; CPD ; CUCUMIS SATIVUS ; Cucumis sativus - genetics ; Cucumis sativus - growth & development ; Cucumis sativus - radiation effects ; cyclobutane pyrimidine dimer ; Deoxyribodipyrimidine Photo-Lyase - genetics ; Deoxyribodipyrimidine Photo-Lyase - physiology ; dithiothreitol ; DNA Repair ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; Dose-Response Relationship, Radiation ; DTT ; ELISA ; enzyme-linked immunosorbent assay ; Escherichia coli - genetics ; GENE EXPRESSION ; Gene Expression Regulation, Enzymologic - radiation effects ; Gene Expression Regulation, Plant - radiation effects ; Genetic Complementation Test ; GROWTH ; Key words: Cucumis sativus — Cyclobutane pyrimidine dimer —Diurnal change — DNA photolyase (EC 4.1.99.3) — Growth retardation —Photorepair ; Light ; LYASES ; Molecular Sequence Data ; Mutation ; Plant Leaves - genetics ; Plant Leaves - growth & development ; Plant Leaves - radiation effects ; Plant Proteins - genetics ; Plant Proteins - physiology ; Pyrimidine Dimers - metabolism ; RACE ; rapid amplification of cDNA ends ; reverse transcription–polymerase chain reaction ; RT-PCR ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; ULTRAVIOLET IRRADIATION ; Ultraviolet Rays</subject><ispartof>Plant and cell physiology, 2002-03, Vol.43 (3), p.342-349</ispartof><rights>Copyright Oxford University Press(England) Mar 15, 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-500427712c1b89a1ba8b5b1e242d1488622c2e64d25378b8c60e8e57cc58744a3</citedby><cites>FETCH-LOGICAL-c492t-500427712c1b89a1ba8b5b1e242d1488622c2e64d25378b8c60e8e57cc58744a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11917089$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, S. (Tokyo Univ. (Japan))</creatorcontrib><creatorcontrib>Nakajima, N</creatorcontrib><creatorcontrib>Saji, H</creatorcontrib><creatorcontrib>Kondo, N</creatorcontrib><title>Diurnal change of cucumber [Cucumis sativus] CPD photolyase gene (CsPHR) expression and its physiological role in growth under UV-B irradiation</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09:00 and 12:00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.</description><subject>Amino Acid Sequence</subject><subject>Circadian Rhythm - physiology</subject><subject>CPD</subject><subject>CUCUMIS SATIVUS</subject><subject>Cucumis sativus - genetics</subject><subject>Cucumis sativus - growth & development</subject><subject>Cucumis sativus - radiation effects</subject><subject>cyclobutane pyrimidine dimer</subject><subject>Deoxyribodipyrimidine Photo-Lyase - genetics</subject><subject>Deoxyribodipyrimidine Photo-Lyase - physiology</subject><subject>dithiothreitol</subject><subject>DNA Repair</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Dose-Response Relationship, Radiation</subject><subject>DTT</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Escherichia coli - genetics</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Enzymologic - radiation effects</subject><subject>Gene Expression Regulation, Plant - radiation effects</subject><subject>Genetic Complementation Test</subject><subject>GROWTH</subject><subject>Key words: Cucumis sativus — Cyclobutane pyrimidine dimer —Diurnal change — DNA photolyase (EC 4.1.99.3) — Growth retardation —Photorepair</subject><subject>Light</subject><subject>LYASES</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - radiation effects</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - physiology</subject><subject>Pyrimidine Dimers - metabolism</subject><subject>RACE</subject><subject>rapid amplification of cDNA ends</subject><subject>reverse transcription–polymerase chain reaction</subject><subject>RT-PCR</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>ULTRAVIOLET IRRADIATION</subject><subject>Ultraviolet Rays</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0e9r1DAYB_AgirtN3_heCb4QFar52aQvXU89x9BDtjEUCWma9jJ7TZe0uvsr_JfN7KEgJCQhH56H5AvAI4xeYVTQ14MZ0mwQlXfAAjOBswJxehcsEKIkQ0LiA3AY4xVCaU_RfXCAcYEFksUC_Fq6KfS6g2aj-9ZC30AzmWlb2QC_lrc7F2HUo_sxxW-wXC_hsPGj73Y6Wtja3sLnZVyvPr-A9mYINkbne6j7GroxJrpL5863zqQOwXcWuh62wf8cN3Dq69Tj_CI7hi4EXbvUxPcPwL1Gd9E-3K9H4Pzd27NylZ1-ev-hfHOaGVaQMeMIMSIEJgZXstC40rLiFbaEkRozKXNCDLE5qwmnQlbS5MhKy4UxXArGND0Cz-a6Q_DXk42jSg81tut0b_0UFZaM5ZyQBJ_-B6_8nx-LiiDMKZMcJfRyRib4GINt1BDcVoedwkjdZqRSRmrOKOEn-4pTtbX1P7oPJYFsBi6O9ubvvQ7fVS6o4Gp1-UWdXR5frJYfsTpJ_vHsG-2VboOL6mRNEEojxwLT31OPpZU</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Takahashi, S. 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(Japan)) ; Nakajima, N ; Saji, H ; Kondo, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-500427712c1b89a1ba8b5b1e242d1488622c2e64d25378b8c60e8e57cc58744a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Circadian Rhythm - physiology</topic><topic>CPD</topic><topic>CUCUMIS SATIVUS</topic><topic>Cucumis sativus - genetics</topic><topic>Cucumis sativus - growth & development</topic><topic>Cucumis sativus - radiation effects</topic><topic>cyclobutane pyrimidine dimer</topic><topic>Deoxyribodipyrimidine Photo-Lyase - genetics</topic><topic>Deoxyribodipyrimidine Photo-Lyase - physiology</topic><topic>dithiothreitol</topic><topic>DNA Repair</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Dose-Response Relationship, Radiation</topic><topic>DTT</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Escherichia coli - genetics</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Enzymologic - radiation effects</topic><topic>Gene Expression Regulation, Plant - radiation effects</topic><topic>Genetic Complementation Test</topic><topic>GROWTH</topic><topic>Key words: Cucumis sativus — Cyclobutane pyrimidine dimer —Diurnal change — DNA photolyase (EC 4.1.99.3) — Growth retardation —Photorepair</topic><topic>Light</topic><topic>LYASES</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - radiation effects</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - physiology</topic><topic>Pyrimidine Dimers - metabolism</topic><topic>RACE</topic><topic>rapid amplification of cDNA ends</topic><topic>reverse transcription–polymerase chain reaction</topic><topic>RT-PCR</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>ULTRAVIOLET IRRADIATION</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, S. 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(Tokyo Univ. (Japan))</au><au>Nakajima, N</au><au>Saji, H</au><au>Kondo, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diurnal change of cucumber [Cucumis sativus] CPD photolyase gene (CsPHR) expression and its physiological role in growth under UV-B irradiation</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>43</volume><issue>3</issue><spage>342</spage><epage>349</epage><pages>342-349</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09:00 and 12:00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>11917089</pmid><doi>10.1093/pcp/pcf038</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Circadian Rhythm - physiology CPD CUCUMIS SATIVUS Cucumis sativus - genetics Cucumis sativus - growth & development Cucumis sativus - radiation effects cyclobutane pyrimidine dimer Deoxyribodipyrimidine Photo-Lyase - genetics Deoxyribodipyrimidine Photo-Lyase - physiology dithiothreitol DNA Repair DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification Dose-Response Relationship, Radiation DTT ELISA enzyme-linked immunosorbent assay Escherichia coli - genetics GENE EXPRESSION Gene Expression Regulation, Enzymologic - radiation effects Gene Expression Regulation, Plant - radiation effects Genetic Complementation Test GROWTH Key words: Cucumis sativus — Cyclobutane pyrimidine dimer —Diurnal change — DNA photolyase (EC 4.1.99.3) — Growth retardation —Photorepair Light LYASES Molecular Sequence Data Mutation Plant Leaves - genetics Plant Leaves - growth & development Plant Leaves - radiation effects Plant Proteins - genetics Plant Proteins - physiology Pyrimidine Dimers - metabolism RACE rapid amplification of cDNA ends reverse transcription–polymerase chain reaction RT-PCR Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid ULTRAVIOLET IRRADIATION Ultraviolet Rays |
| title | Diurnal change of cucumber [Cucumis sativus] CPD photolyase gene (CsPHR) expression and its physiological role in growth under UV-B irradiation |
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