Genotoxic effect of ozone in human peripheral blood leukocytes
The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1 h at 37 °C with 0.9–5.3 mM O 3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40 mM of H 2O 2, used as a positive...
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| Veröffentlicht in: | Mutation research 2002-05, Vol.517 (1), p.13-20 |
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| creator | Dı́az-Llera, Silvia González-Hernández, Yanela Prieto-González, E.A Azoy, Angel |
| description | The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1
h at 37
°C with 0.9–5.3
mM O
3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40
mM of H
2O
2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45–90
min at 37
°C, and prevented by pre-incubation of the cells with catalase (20
μg/ml). These results demonstrate that O
3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H
2O
2. |
| doi_str_mv | 10.1016/S1383-5718(02)00022-0 |
| format | Article |
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h at 37
°C with 0.9–5.3
mM O
3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40
mM of H
2O
2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45–90
min at 37
°C, and prevented by pre-incubation of the cells with catalase (20
μg/ml). These results demonstrate that O
3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H
2O
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h at 37
°C with 0.9–5.3
mM O
3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40
mM of H
2O
2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45–90
min at 37
°C, and prevented by pre-incubation of the cells with catalase (20
μg/ml). These results demonstrate that O
3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H
2O
2.</description><subject>Biological and medical sciences</subject><subject>Catalase</subject><subject>Catalase - therapeutic use</subject><subject>Cells, Cultured</subject><subject>Comet Assay</subject><subject>DNA Damage</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Human leukocytes</subject><subject>Humans</subject><subject>Leukocytes - metabolism</subject><subject>Medical sciences</subject><subject>Miscellaneous (drug allergy, mutagens, teratogens...)</subject><subject>Oxygen - pharmacology</subject><subject>Ozone</subject><subject>Ozone - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>SCGE</subject><issn>1383-5718</issn><issn>0027-5107</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EolD4BFA2IFgE_EribkCogoJUiQWwthxnrBqSuNgJonw97gN1yWru4szc0UHohOArgkl-_UKYYGlWEHGB6SXGmNIU76ADIopRyrIR3Y35DxmgwxDeI4MZFvtoQGLgDPMDdDOB1nXu2-oEjAHdJc4k7se1kNg2mfWNapM5eDufgVd1UtbOVUkN_YfTiw7CEdozqg5wvJlD9PZw_zp-TKfPk6fx3TTVnNIu1YozKLnJiQAzMkbQTKs846ZgYFhFcCXKQuW6AGLyjBagBaVgtCgywkcMsyE6X9-de_fZQ-hkY4OGulYtuD5IIjjPWS4imK1B7V0IHoyce9sov5AEy6U4uRInl1YkpnIlTi4LTjcFfdlAtd3amIrA2QZQQavaeNVqG7ZcLI-fLw_drjmIOr4seBm0hVZDZX3UKytn_3nlFxtcic0</recordid><startdate>20020527</startdate><enddate>20020527</enddate><creator>Dı́az-Llera, Silvia</creator><creator>González-Hernández, Yanela</creator><creator>Prieto-González, E.A</creator><creator>Azoy, Angel</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20020527</creationdate><title>Genotoxic effect of ozone in human peripheral blood leukocytes</title><author>Dı́az-Llera, Silvia ; González-Hernández, Yanela ; Prieto-González, E.A ; Azoy, Angel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-ca43eb4f618ef9ff825ca654f73ef3d10d8b7a6c7e1f6527ec822efc875149303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Catalase</topic><topic>Catalase - therapeutic use</topic><topic>Cells, Cultured</topic><topic>Comet Assay</topic><topic>DNA Damage</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Human leukocytes</topic><topic>Humans</topic><topic>Leukocytes - metabolism</topic><topic>Medical sciences</topic><topic>Miscellaneous (drug allergy, mutagens, teratogens...)</topic><topic>Oxygen - pharmacology</topic><topic>Ozone</topic><topic>Ozone - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>SCGE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dı́az-Llera, Silvia</creatorcontrib><creatorcontrib>González-Hernández, Yanela</creatorcontrib><creatorcontrib>Prieto-González, E.A</creatorcontrib><creatorcontrib>Azoy, Angel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Mutation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dı́az-Llera, Silvia</au><au>González-Hernández, Yanela</au><au>Prieto-González, E.A</au><au>Azoy, Angel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxic effect of ozone in human peripheral blood leukocytes</atitle><jtitle>Mutation research</jtitle><addtitle>Mutat Res</addtitle><date>2002-05-27</date><risdate>2002</risdate><volume>517</volume><issue>1</issue><spage>13</spage><epage>20</epage><pages>13-20</pages><issn>1383-5718</issn><issn>0027-5107</issn><eissn>1879-3592</eissn><abstract>The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1
h at 37
°C with 0.9–5.3
mM O
3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40
mM of H
2O
2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45–90
min at 37
°C, and prevented by pre-incubation of the cells with catalase (20
μg/ml). These results demonstrate that O
3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H
2O
2.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12034304</pmid><doi>10.1016/S1383-5718(02)00022-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Mutation research, 2002-05, Vol.517 (1), p.13-20 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biological and medical sciences Catalase Catalase - therapeutic use Cells, Cultured Comet Assay DNA Damage Dose-Response Relationship, Drug Drug toxicity and drugs side effects treatment Human leukocytes Humans Leukocytes - metabolism Medical sciences Miscellaneous (drug allergy, mutagens, teratogens...) Oxygen - pharmacology Ozone Ozone - pharmacology Pharmacology. Drug treatments SCGE |
| title | Genotoxic effect of ozone in human peripheral blood leukocytes |
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