Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas
MicroRNA (miR)-497 plays a tumor-suppressive role in several malignancies and is involved in glioma invasiveness and resistance to chemotherapy. To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction (qRT-PCR) was performed...
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| Veröffentlicht in: | Human pathology 2016-12, Vol.58, p.47-53 |
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| creator | Feng, Fuqiang, MD Kuai, Dong, MD Wang, Hongqin, MS Li, Tao, MS Miao, Wang, MS Liu, Yueting, MS Fan, Yimin, MD |
| description | MicroRNA (miR)-497 plays a tumor-suppressive role in several malignancies and is involved in glioma invasiveness and resistance to chemotherapy. To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-497 in 110 pairs of freshly prepared glioma and nonneoplastic brain tissues. Then the associations of miR-497 expression with various clinicopathologic characteristics and overall survival of glioma patients were estimated statistically. Gain-of-function assays also were performed to examine the role of miR-497 in glioma angiogenesis. The expression of miR-497 in human glioma tissues was significantly lower than in nonneoplastic brain tissues (P < .001). In addition, low miR-497 expression was significantly associated with advanced World Health Organization grade (P < .001) and low Karnofsky performance scores (P = .02). Moreover, the survival of glioma patients with low miR-497 expression was dramatically shorter than that of patients with high miR-497 expression (P = .001). Forced expression of miR-497 in glioma cells inhibited tube formation by co-cultured human brain microvascular endothelial cells. We also found that miR-497 overexpression in glioma cells led to decreased expression of vascular endothelial growth factor (VEGF). In conclusion, miR-497 may be a favorable prognostic marker in human gliomas, in part by being a negative regulator of angiogenesis, implying its potential as a therapeutic target for this cancer. |
| doi_str_mv | 10.1016/j.humpath.2016.04.022 |
| format | Article |
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To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-497 in 110 pairs of freshly prepared glioma and nonneoplastic brain tissues. Then the associations of miR-497 expression with various clinicopathologic characteristics and overall survival of glioma patients were estimated statistically. Gain-of-function assays also were performed to examine the role of miR-497 in glioma angiogenesis. The expression of miR-497 in human glioma tissues was significantly lower than in nonneoplastic brain tissues (P < .001). In addition, low miR-497 expression was significantly associated with advanced World Health Organization grade (P < .001) and low Karnofsky performance scores (P = .02). Moreover, the survival of glioma patients with low miR-497 expression was dramatically shorter than that of patients with high miR-497 expression (P = .001). Forced expression of miR-497 in glioma cells inhibited tube formation by co-cultured human brain microvascular endothelial cells. We also found that miR-497 overexpression in glioma cells led to decreased expression of vascular endothelial growth factor (VEGF). In conclusion, miR-497 may be a favorable prognostic marker in human gliomas, in part by being a negative regulator of angiogenesis, implying its potential as a therapeutic target for this cancer.</description><identifier>ISSN: 0046-8177</identifier><identifier>EISSN: 1532-8392</identifier><identifier>DOI: 10.1016/j.humpath.2016.04.022</identifier><identifier>PMID: 27569294</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Angiogenesis ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Brain cancer ; Brain Neoplasms - blood supply ; Brain Neoplasms - genetics ; Brain Neoplasms - metabolism ; Brain Neoplasms - pathology ; Brain research ; Cancer ; Cell Line, Tumor ; Child ; Clinicopathological features ; Coculture Techniques ; Down-Regulation ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Glioma ; Glioma - blood supply ; Glioma - genetics ; Glioma - metabolism ; Glioma - pathology ; Hospitals ; Humans ; Kaplan-Meier Estimate ; Karnofsky Performance Status ; Male ; Medical prognosis ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miR-497 ; Multivariate analysis ; Neoplasm Grading ; Neovascularization, Pathologic ; Pathology ; Prognosis ; Real-Time Polymerase Chain Reaction ; Transfection ; Tumors ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; Young Adult</subject><ispartof>Human pathology, 2016-12, Vol.58, p.47-53</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier Limited Dec 01, 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-13255d9a1f9e54fe464fef8d2a07049ad57cc38b5cea93047ed8cadc38a72fb53</citedby><cites>FETCH-LOGICAL-c448t-13255d9a1f9e54fe464fef8d2a07049ad57cc38b5cea93047ed8cadc38a72fb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.humpath.2016.04.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27569294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Fuqiang, MD</creatorcontrib><creatorcontrib>Kuai, Dong, MD</creatorcontrib><creatorcontrib>Wang, Hongqin, MS</creatorcontrib><creatorcontrib>Li, Tao, MS</creatorcontrib><creatorcontrib>Miao, Wang, MS</creatorcontrib><creatorcontrib>Liu, Yueting, MS</creatorcontrib><creatorcontrib>Fan, Yimin, MD</creatorcontrib><title>Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas</title><title>Human pathology</title><addtitle>Hum Pathol</addtitle><description>MicroRNA (miR)-497 plays a tumor-suppressive role in several malignancies and is involved in glioma invasiveness and resistance to chemotherapy. To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-497 in 110 pairs of freshly prepared glioma and nonneoplastic brain tissues. Then the associations of miR-497 expression with various clinicopathologic characteristics and overall survival of glioma patients were estimated statistically. Gain-of-function assays also were performed to examine the role of miR-497 in glioma angiogenesis. The expression of miR-497 in human glioma tissues was significantly lower than in nonneoplastic brain tissues (P < .001). In addition, low miR-497 expression was significantly associated with advanced World Health Organization grade (P < .001) and low Karnofsky performance scores (P = .02). Moreover, the survival of glioma patients with low miR-497 expression was dramatically shorter than that of patients with high miR-497 expression (P = .001). Forced expression of miR-497 in glioma cells inhibited tube formation by co-cultured human brain microvascular endothelial cells. We also found that miR-497 overexpression in glioma cells led to decreased expression of vascular endothelial growth factor (VEGF). In conclusion, miR-497 may be a favorable prognostic marker in human gliomas, in part by being a negative regulator of angiogenesis, implying its potential as a therapeutic target for this cancer.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Angiogenesis</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Brain cancer</subject><subject>Brain Neoplasms - blood supply</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - metabolism</subject><subject>Brain Neoplasms - pathology</subject><subject>Brain research</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Child</subject><subject>Clinicopathological features</subject><subject>Coculture Techniques</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Glioma</subject><subject>Glioma - blood supply</subject><subject>Glioma - genetics</subject><subject>Glioma - metabolism</subject><subject>Glioma - pathology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Karnofsky Performance Status</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miR-497</subject><subject>Multivariate analysis</subject><subject>Neoplasm Grading</subject><subject>Neovascularization, Pathologic</subject><subject>Pathology</subject><subject>Prognosis</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Young Adult</subject><issn>0046-8177</issn><issn>1532-8392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi1ERbeFnwCyxIVLUtuxk_gCqiooSFWRCpwtrz3JeknsYCdA_30d7QJSL1xszeiZz3cQeklJSQmtL_blbhknPe9Kls2S8JIw9gRtqKhY0VaSPUUbQnhdtLRpTtFZSntCKBVcPEOnrBG1ZJJvkL8DuxiwGH5PEVJywePQ4dGZGO5uLwsuG-wS1ikF4_ScwV9u3uE-QjYi1r53oQcPaYW8xVMIEU8x9D6sLudxblN73A8ujDo9RyedHhK8OP7n6NuH91-vPhY3n68_XV3eFIbzdi5oxYSwUtNOguAd8Do_XWuZJg3hUlvRGFO1W2FAy4rwBmxrtM0u3bBuK6pz9OaQN7fyY4E0q9ElA8OgPYQlKdpyXgnatjSjrx-h-7BEn7tbqYZzyiXJlDhQeS8pRejUFN2o472iRK2CqL06CqJWQRThKguS414dsy_bEezfqD8KZODdAYC8jp8OokrGgc-SuAhmVja4_5Z4-yiDGZx3Rg_f4R7Sv2lUYoqoL-tVrEdB64rQPFr1AONytLk</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Feng, Fuqiang, MD</creator><creator>Kuai, Dong, MD</creator><creator>Wang, Hongqin, MS</creator><creator>Li, Tao, MS</creator><creator>Miao, Wang, MS</creator><creator>Liu, Yueting, MS</creator><creator>Fan, Yimin, MD</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20161201</creationdate><title>Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas</title><author>Feng, Fuqiang, MD ; Kuai, Dong, MD ; Wang, Hongqin, MS ; Li, Tao, MS ; Miao, Wang, MS ; Liu, Yueting, MS ; Fan, Yimin, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-13255d9a1f9e54fe464fef8d2a07049ad57cc38b5cea93047ed8cadc38a72fb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Angiogenesis</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Brain cancer</topic><topic>Brain Neoplasms - blood supply</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - metabolism</topic><topic>Brain Neoplasms - pathology</topic><topic>Brain research</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Child</topic><topic>Clinicopathological features</topic><topic>Coculture Techniques</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Glioma</topic><topic>Glioma - blood supply</topic><topic>Glioma - genetics</topic><topic>Glioma - metabolism</topic><topic>Glioma - pathology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Karnofsky Performance Status</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miR-497</topic><topic>Multivariate analysis</topic><topic>Neoplasm Grading</topic><topic>Neovascularization, Pathologic</topic><topic>Pathology</topic><topic>Prognosis</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Transfection</topic><topic>Tumors</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Fuqiang, MD</creatorcontrib><creatorcontrib>Kuai, Dong, MD</creatorcontrib><creatorcontrib>Wang, Hongqin, MS</creatorcontrib><creatorcontrib>Li, Tao, MS</creatorcontrib><creatorcontrib>Miao, Wang, MS</creatorcontrib><creatorcontrib>Liu, Yueting, MS</creatorcontrib><creatorcontrib>Fan, Yimin, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Human pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Fuqiang, MD</au><au>Kuai, Dong, MD</au><au>Wang, Hongqin, MS</au><au>Li, Tao, MS</au><au>Miao, Wang, MS</au><au>Liu, Yueting, MS</au><au>Fan, Yimin, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas</atitle><jtitle>Human pathology</jtitle><addtitle>Hum Pathol</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>58</volume><spage>47</spage><epage>53</epage><pages>47-53</pages><issn>0046-8177</issn><eissn>1532-8392</eissn><abstract>MicroRNA (miR)-497 plays a tumor-suppressive role in several malignancies and is involved in glioma invasiveness and resistance to chemotherapy. To add to the knowledge of the clinical significance of miR-497 in human gliomas, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miR-497 in 110 pairs of freshly prepared glioma and nonneoplastic brain tissues. Then the associations of miR-497 expression with various clinicopathologic characteristics and overall survival of glioma patients were estimated statistically. Gain-of-function assays also were performed to examine the role of miR-497 in glioma angiogenesis. The expression of miR-497 in human glioma tissues was significantly lower than in nonneoplastic brain tissues (P < .001). In addition, low miR-497 expression was significantly associated with advanced World Health Organization grade (P < .001) and low Karnofsky performance scores (P = .02). Moreover, the survival of glioma patients with low miR-497 expression was dramatically shorter than that of patients with high miR-497 expression (P = .001). Forced expression of miR-497 in glioma cells inhibited tube formation by co-cultured human brain microvascular endothelial cells. We also found that miR-497 overexpression in glioma cells led to decreased expression of vascular endothelial growth factor (VEGF). In conclusion, miR-497 may be a favorable prognostic marker in human gliomas, in part by being a negative regulator of angiogenesis, implying its potential as a therapeutic target for this cancer.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27569294</pmid><doi>10.1016/j.humpath.2016.04.022</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Angiogenesis Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Brain cancer Brain Neoplasms - blood supply Brain Neoplasms - genetics Brain Neoplasms - metabolism Brain Neoplasms - pathology Brain research Cancer Cell Line, Tumor Child Clinicopathological features Coculture Techniques Down-Regulation Female Gene expression Gene Expression Regulation, Neoplastic Glioma Glioma - blood supply Glioma - genetics Glioma - metabolism Glioma - pathology Hospitals Humans Kaplan-Meier Estimate Karnofsky Performance Status Male Medical prognosis MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miR-497 Multivariate analysis Neoplasm Grading Neovascularization, Pathologic Pathology Prognosis Real-Time Polymerase Chain Reaction Transfection Tumors Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism Young Adult |
| title | Reduced expression of microRNA-497 is associated with greater angiogenesis and poor prognosis in human gliomas |
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