Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia
BACKGROUND Myzus persicae is a serious pest that attacks a broad range of agricultural crops. This species has developed chemical resistance to many insecticides globally, and within Australia resistance to multiple chemical groups has been identified. Resistance to neonicotinoid insecticides has be...
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| Veröffentlicht in: | Pest management science 2017-08, Vol.73 (8), p.1611-1617 |
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| creator | de Little, Siobhan C Edwards, Owain van Rooyen, Anthony R Weeks, Andrew Umina, Paul A |
| description | BACKGROUND
Myzus persicae is a serious pest that attacks a broad range of agricultural crops. This species has developed chemical resistance to many insecticides globally, and within Australia resistance to multiple chemical groups has been identified. Resistance to neonicotinoid insecticides has been discovered in several countries, but has not previously been confirmed in Australia. We use biomolecular assays and bioassays on field‐collected populations to investigate neonicotinoid resistance in M. persicae within Australia.
RESULTS
Several geographically and genetically distinct populations showed evidence for resistance in bioassays. Genetic markers identified that the mechanism of neonicotinoid resistance in Australia is metabolic resistance through the enhanced expression of a cytochrome P450 gene, CYP6CY3.
CONCLUSION
M. persicae populations in parts of Australia are now resistant to four different insecticide chemical groups, raising concerns about the long‐term management of this pest. While higher copy numbers of CYP6CY3 were seen in all resistant populations, the number of gene copies was not strongly correlated with the level of resistance as determined by LD50 values generated through bioassays. This finding sheds further light on the complexity of the P450 genes in regulating neonicotinoid resistance. © 2016 Society of Chemical Industry |
| doi_str_mv | 10.1002/ps.4495 |
| format | Article |
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Myzus persicae is a serious pest that attacks a broad range of agricultural crops. This species has developed chemical resistance to many insecticides globally, and within Australia resistance to multiple chemical groups has been identified. Resistance to neonicotinoid insecticides has been discovered in several countries, but has not previously been confirmed in Australia. We use biomolecular assays and bioassays on field‐collected populations to investigate neonicotinoid resistance in M. persicae within Australia.
RESULTS
Several geographically and genetically distinct populations showed evidence for resistance in bioassays. Genetic markers identified that the mechanism of neonicotinoid resistance in Australia is metabolic resistance through the enhanced expression of a cytochrome P450 gene, CYP6CY3.
CONCLUSION
M. persicae populations in parts of Australia are now resistant to four different insecticide chemical groups, raising concerns about the long‐term management of this pest. While higher copy numbers of CYP6CY3 were seen in all resistant populations, the number of gene copies was not strongly correlated with the level of resistance as determined by LD50 values generated through bioassays. This finding sheds further light on the complexity of the P450 genes in regulating neonicotinoid resistance. © 2016 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.4495</identifier><identifier>PMID: 27888606</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Agricultural management ; Agrochemicals ; Animals ; Aphids - genetics ; Aphids - metabolism ; Australia ; Bioassays ; Biological Assay ; Chemical attack ; Corrosion resistance ; Cytochrome P450 ; Gene expression ; Genetic markers ; Genotyping Techniques ; Insecticide resistance ; Insecticide Resistance - genetics ; Insecticides ; Metabolism ; Myzus persicae ; neonicotinoid ; Neonicotinoid insecticides ; Neonicotinoids ; P450 gene ; Pesticides ; Pests ; Population genetics ; Populations</subject><ispartof>Pest management science, 2017-08, Vol.73 (8), p.1611-1617</ispartof><rights>2016 Society of Chemical Industry</rights><rights>2016 Society of Chemical Industry.</rights><rights>2017 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4445-b55c84229301cc2c1b78a3f5475aa822c4476a7de0040501aa6f889ca1ad95433</citedby><cites>FETCH-LOGICAL-c4445-b55c84229301cc2c1b78a3f5475aa822c4476a7de0040501aa6f889ca1ad95433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.4495$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.4495$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27888606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Little, Siobhan C</creatorcontrib><creatorcontrib>Edwards, Owain</creatorcontrib><creatorcontrib>van Rooyen, Anthony R</creatorcontrib><creatorcontrib>Weeks, Andrew</creatorcontrib><creatorcontrib>Umina, Paul A</creatorcontrib><title>Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Myzus persicae is a serious pest that attacks a broad range of agricultural crops. This species has developed chemical resistance to many insecticides globally, and within Australia resistance to multiple chemical groups has been identified. Resistance to neonicotinoid insecticides has been discovered in several countries, but has not previously been confirmed in Australia. We use biomolecular assays and bioassays on field‐collected populations to investigate neonicotinoid resistance in M. persicae within Australia.
RESULTS
Several geographically and genetically distinct populations showed evidence for resistance in bioassays. Genetic markers identified that the mechanism of neonicotinoid resistance in Australia is metabolic resistance through the enhanced expression of a cytochrome P450 gene, CYP6CY3.
CONCLUSION
M. persicae populations in parts of Australia are now resistant to four different insecticide chemical groups, raising concerns about the long‐term management of this pest. While higher copy numbers of CYP6CY3 were seen in all resistant populations, the number of gene copies was not strongly correlated with the level of resistance as determined by LD50 values generated through bioassays. This finding sheds further light on the complexity of the P450 genes in regulating neonicotinoid resistance. © 2016 Society of Chemical Industry</description><subject>Agricultural management</subject><subject>Agrochemicals</subject><subject>Animals</subject><subject>Aphids - genetics</subject><subject>Aphids - metabolism</subject><subject>Australia</subject><subject>Bioassays</subject><subject>Biological Assay</subject><subject>Chemical attack</subject><subject>Corrosion resistance</subject><subject>Cytochrome P450</subject><subject>Gene expression</subject><subject>Genetic markers</subject><subject>Genotyping Techniques</subject><subject>Insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides</subject><subject>Metabolism</subject><subject>Myzus persicae</subject><subject>neonicotinoid</subject><subject>Neonicotinoid insecticides</subject><subject>Neonicotinoids</subject><subject>P450 gene</subject><subject>Pesticides</subject><subject>Pests</subject><subject>Population genetics</subject><subject>Populations</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MtKAzEUBuAgivWGbyABF1akmmSSmWRZ6hUqCiq4C6dpRiPTyZjMKPXpTa26EFwlHL785PwI7VJyTAlhJ0085lyJFbRBBcsHXCm5-nuXjz20GeMLIUQpxdZRjxVSypzkG-jp1EXj32yYY1_imW1h4itncLDRxRZqY3HrcW197YxvXe3dNGJX46dgbY0bC-YZQ_O8mPav5x9dTLMQnQF7uGDDLrYBKgfbaK2EKtqd73MLPZyf3Y8uB-Obi6vRcDwwnHMxmAhhJGdMZYQawwydFBKyUvBCAEjGkipyKKaWEE4EoQB5KaUyQGGqBM-yLdRf5jbBv3Y2tnqWFrRVBWmHLmoqOSdMpdeJ7v-hL74LdfqdpipVx4tM0aQOlsoEH2OwpW6Cm0GYa0r0onvdRL3oPsm977xuMrPTX_dTdgJHS_DuKjv_L0ff3n3FfQLYZIvA</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>de Little, Siobhan C</creator><creator>Edwards, Owain</creator><creator>van Rooyen, Anthony R</creator><creator>Weeks, Andrew</creator><creator>Umina, Paul A</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia</title><author>de Little, Siobhan C ; Edwards, Owain ; van Rooyen, Anthony R ; Weeks, Andrew ; Umina, Paul A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4445-b55c84229301cc2c1b78a3f5475aa822c4476a7de0040501aa6f889ca1ad95433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Agricultural management</topic><topic>Agrochemicals</topic><topic>Animals</topic><topic>Aphids - genetics</topic><topic>Aphids - metabolism</topic><topic>Australia</topic><topic>Bioassays</topic><topic>Biological Assay</topic><topic>Chemical attack</topic><topic>Corrosion resistance</topic><topic>Cytochrome P450</topic><topic>Gene expression</topic><topic>Genetic markers</topic><topic>Genotyping Techniques</topic><topic>Insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>Insecticides</topic><topic>Metabolism</topic><topic>Myzus persicae</topic><topic>neonicotinoid</topic><topic>Neonicotinoid insecticides</topic><topic>Neonicotinoids</topic><topic>P450 gene</topic><topic>Pesticides</topic><topic>Pests</topic><topic>Population genetics</topic><topic>Populations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Little, Siobhan C</creatorcontrib><creatorcontrib>Edwards, Owain</creatorcontrib><creatorcontrib>van Rooyen, Anthony R</creatorcontrib><creatorcontrib>Weeks, Andrew</creatorcontrib><creatorcontrib>Umina, Paul A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Little, Siobhan C</au><au>Edwards, Owain</au><au>van Rooyen, Anthony R</au><au>Weeks, Andrew</au><au>Umina, Paul A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2017-08</date><risdate>2017</risdate><volume>73</volume><issue>8</issue><spage>1611</spage><epage>1617</epage><pages>1611-1617</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Myzus persicae is a serious pest that attacks a broad range of agricultural crops. This species has developed chemical resistance to many insecticides globally, and within Australia resistance to multiple chemical groups has been identified. Resistance to neonicotinoid insecticides has been discovered in several countries, but has not previously been confirmed in Australia. We use biomolecular assays and bioassays on field‐collected populations to investigate neonicotinoid resistance in M. persicae within Australia.
RESULTS
Several geographically and genetically distinct populations showed evidence for resistance in bioassays. Genetic markers identified that the mechanism of neonicotinoid resistance in Australia is metabolic resistance through the enhanced expression of a cytochrome P450 gene, CYP6CY3.
CONCLUSION
M. persicae populations in parts of Australia are now resistant to four different insecticide chemical groups, raising concerns about the long‐term management of this pest. While higher copy numbers of CYP6CY3 were seen in all resistant populations, the number of gene copies was not strongly correlated with the level of resistance as determined by LD50 values generated through bioassays. This finding sheds further light on the complexity of the P450 genes in regulating neonicotinoid resistance. © 2016 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>27888606</pmid><doi>10.1002/ps.4495</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Agricultural management Agrochemicals Animals Aphids - genetics Aphids - metabolism Australia Bioassays Biological Assay Chemical attack Corrosion resistance Cytochrome P450 Gene expression Genetic markers Genotyping Techniques Insecticide resistance Insecticide Resistance - genetics Insecticides Metabolism Myzus persicae neonicotinoid Neonicotinoid insecticides Neonicotinoids P450 gene Pesticides Pests Population genetics Populations |
| title | Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia |
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