Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats
This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duode...
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| Veröffentlicht in: | Acta histochemica 2017-01, Vol.119 (1), p.26-31 |
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| container_title | Acta histochemica |
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| creator | Zhou, Ying Yuan, Huai-rui Cui, Lu Ansari, Abdur Rahman Xiao, Ke Luo, You Wu, Xin-tong Guo, Liang Khan, Faheem Ahmed Yang, Zhi Song, Hui |
| description | This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation. |
| doi_str_mv | 10.1016/j.acthis.2016.11.002 |
| format | Article |
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Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/j.acthis.2016.11.002</identifier><identifier>PMID: 27884396</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Caspase 3 - genetics ; Caspase 3 - metabolism ; Caspase-3 ; Drug Combinations ; Duodenum - cytology ; Duodenum - drug effects ; Duodenum - immunology ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - immunology ; Gene Expression ; Ileum - cytology ; Ileum - drug effects ; Ileum - immunology ; Immunity, Mucosal - drug effects ; In Situ Nick-End Labeling ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - immunology ; Intestine mucosal ; Jejunum - cytology ; Jejunum - drug effects ; Jejunum - immunology ; Lipopolysaccharides - pharmacology ; LPS induced ; Nicotinamide Phosphoribosyltransferase - pharmacology ; Rats ; Rats, Wistar ; Visfatin</subject><ispartof>Acta histochemica, 2017-01, Vol.119 (1), p.26-31</ispartof><rights>2016 Elsevier GmbH</rights><rights>Copyright © 2016 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-dce5054179c8c5968ff69a90a7031f175ad7899f37584f283800794c3f71ea843</citedby><cites>FETCH-LOGICAL-c362t-dce5054179c8c5968ff69a90a7031f175ad7899f37584f283800794c3f71ea843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.acthis.2016.11.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27884396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Yuan, Huai-rui</creatorcontrib><creatorcontrib>Cui, Lu</creatorcontrib><creatorcontrib>Ansari, Abdur Rahman</creatorcontrib><creatorcontrib>Xiao, Ke</creatorcontrib><creatorcontrib>Luo, You</creatorcontrib><creatorcontrib>Wu, Xin-tong</creatorcontrib><creatorcontrib>Guo, Liang</creatorcontrib><creatorcontrib>Khan, Faheem Ahmed</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><creatorcontrib>Song, Hui</creatorcontrib><title>Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Caspase 3 - genetics</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase-3</subject><subject>Drug Combinations</subject><subject>Duodenum - cytology</subject><subject>Duodenum - drug effects</subject><subject>Duodenum - immunology</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - immunology</subject><subject>Gene Expression</subject><subject>Ileum - cytology</subject><subject>Ileum - drug effects</subject><subject>Ileum - immunology</subject><subject>Immunity, Mucosal - drug effects</subject><subject>In Situ Nick-End Labeling</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestine mucosal</subject><subject>Jejunum - cytology</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - immunology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>LPS induced</subject><subject>Nicotinamide Phosphoribosyltransferase - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Visfatin</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhq0KRLct_wChHLkkzNhJbF-QUFUoUiUucLZcx269SuLF41Ti39fLFo6cRjPvO18PY-8QOgQcP-4768pjpI7XrEPsAPgZ2-GIqgUh-Su2AxiHFrnCc3ZBtAcADYK_YedcKtULPe6YuQnBu0JNCs1TpGBLXJu0NuXRN_aQDiVR_CPGtXiqop2bZXOJanR-nqkKTVyWbU1zeoiulqlkT-SnJttCV-x1sDP5ty_xkv38cvPj-ra9-_712_Xnu9aJkZd2cn6AoUepnXKDHlUIo7YarASBAeVgJ6m0DkIOqg9cCQUgde9EkOht_eWSfTjNPeT0a6uXmiXS8UC7-rSRQdX3wCXXolr7k9XlRJR9MIccF5t_GwRzRGv25oTWHNEaRFPR1rb3Lxu2-8VP_5r-sqyGTyeDr38-RZ8NuehX56eYK2Izpfj_Dc92Loxf</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Zhou, Ying</creator><creator>Yuan, Huai-rui</creator><creator>Cui, Lu</creator><creator>Ansari, Abdur Rahman</creator><creator>Xiao, Ke</creator><creator>Luo, You</creator><creator>Wu, Xin-tong</creator><creator>Guo, Liang</creator><creator>Khan, Faheem Ahmed</creator><creator>Yang, Zhi</creator><creator>Song, Hui</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats</title><author>Zhou, Ying ; Yuan, Huai-rui ; Cui, Lu ; Ansari, Abdur Rahman ; Xiao, Ke ; Luo, You ; Wu, Xin-tong ; Guo, Liang ; Khan, Faheem Ahmed ; Yang, Zhi ; Song, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-dce5054179c8c5968ff69a90a7031f175ad7899f37584f283800794c3f71ea843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Caspase 3 - genetics</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase-3</topic><topic>Drug Combinations</topic><topic>Duodenum - cytology</topic><topic>Duodenum - drug effects</topic><topic>Duodenum - immunology</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - immunology</topic><topic>Gene Expression</topic><topic>Ileum - cytology</topic><topic>Ileum - drug effects</topic><topic>Ileum - immunology</topic><topic>Immunity, Mucosal - drug effects</topic><topic>In Situ Nick-End Labeling</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestine mucosal</topic><topic>Jejunum - cytology</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - immunology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>LPS induced</topic><topic>Nicotinamide Phosphoribosyltransferase - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Visfatin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Yuan, Huai-rui</creatorcontrib><creatorcontrib>Cui, Lu</creatorcontrib><creatorcontrib>Ansari, Abdur Rahman</creatorcontrib><creatorcontrib>Xiao, Ke</creatorcontrib><creatorcontrib>Luo, You</creatorcontrib><creatorcontrib>Wu, Xin-tong</creatorcontrib><creatorcontrib>Guo, Liang</creatorcontrib><creatorcontrib>Khan, Faheem Ahmed</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><creatorcontrib>Song, Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Ying</au><au>Yuan, Huai-rui</au><au>Cui, Lu</au><au>Ansari, Abdur Rahman</au><au>Xiao, Ke</au><au>Luo, You</au><au>Wu, Xin-tong</au><au>Guo, Liang</au><au>Khan, Faheem Ahmed</au><au>Yang, Zhi</au><au>Song, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2017-01</date><risdate>2017</risdate><volume>119</volume><issue>1</issue><spage>26</spage><epage>31</epage><pages>26-31</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>27884396</pmid><doi>10.1016/j.acthis.2016.11.002</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Apoptosis Apoptosis - drug effects Caspase 3 - genetics Caspase 3 - metabolism Caspase-3 Drug Combinations Duodenum - cytology Duodenum - drug effects Duodenum - immunology Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - immunology Gene Expression Ileum - cytology Ileum - drug effects Ileum - immunology Immunity, Mucosal - drug effects In Situ Nick-End Labeling Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - immunology Intestine mucosal Jejunum - cytology Jejunum - drug effects Jejunum - immunology Lipopolysaccharides - pharmacology LPS induced Nicotinamide Phosphoribosyltransferase - pharmacology Rats Rats, Wistar Visfatin |
| title | Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats |
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