Mass Spectrometry-based Methods for Phosphorylation Site Mapping of Hyperphosphorylated Proteins Applied to Net1, a Regulator of Exit from Mitosis in Yeast

Prior to anaphase in Saccharomyces cerevisiae , Cdc14 protein phosphatase is sequestered within the nucleolus and inhibited by Net1, a component of the RENT complex in budding yeast. During anaphase the RENT complex disassembles, allowing Cdc14 to migrate to the nucleus and cytoplasm where it cataly...

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Veröffentlicht in:	Molecular & cellular proteomics 2002-03, Vol.1 (3), p.186-196
Hauptverfasser:	Loughrey Chen, Susan, Huddleston, Michael J, Shou, Wenying, Deshaies, Raymond J, Annan, Roland S, Carr, Steven A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Binding Sites Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Chromatography, Liquid Mass Spectrometry - methods Mitosis Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins - chemistry Nuclear Proteins - genetics Peptide Mapping - methods Phosphorylation Proteome - chemistry Proteome - genetics Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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