Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography

The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-boun...

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Veröffentlicht in:	Analytical biochemistry 2017-01, Vol.517, p.53-55
Hauptverfasser:	Baranovskaya, Marianna D., Ugarov, Victor I., Chetverina, Helena V., Chetverin, Alexander B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugation Centrifugation, Density Gradient - methods Chromatography, Affinity E. coli ribosomes Escherichia coli - chemistry Escherichia coli Proteins - isolation & purification Poly U - chemistry Poly(C) Poly(U) Protein S1 Ribosomal Proteins - isolation & purification Ribosomes - chemistry Sucrose - analogs & derivatives Sucrose - chemistry
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