Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography
The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-boun...
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| Veröffentlicht in: | Analytical biochemistry 2017-01, Vol.517, p.53-55 |
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| container_title | Analytical biochemistry |
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| creator | Baranovskaya, Marianna D. Ugarov, Victor I. Chetverina, Helena V. Chetverin, Alexander B. |
| description | The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-bound polypyrimidine with the ribosomes, its length should not exceed several hundred nucleotides. Unlike popular affinity chromatography through a poly(U) Sepharose or poly(U) cellulose column, the method tolerates limited polyribonucleotide degradation by eventual traces of ribonucleases, and can readily be incorporated into standard protocols for the isolation of ribosomes by centrifugation.
•S1 is removed from ribosomes by centrifugation following incubation with poly(C) or poly(U).•Size matters: Optimally, the pyrimidine polyribonucleotide is 100–200 nt long.•The treatment is compatible with standard ribosome washing procedures.•The expensive affinity resin is no longer needed. |
| doi_str_mv | 10.1016/j.ab.2016.11.010 |
| format | Article |
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•S1 is removed from ribosomes by centrifugation following incubation with poly(C) or poly(U).•Size matters: Optimally, the pyrimidine polyribonucleotide is 100–200 nt long.•The treatment is compatible with standard ribosome washing procedures.•The expensive affinity resin is no longer needed.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2016.11.010</identifier><identifier>PMID: 27865825</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Centrifugation ; Centrifugation, Density Gradient - methods ; Chromatography, Affinity ; E. coli ribosomes ; Escherichia coli - chemistry ; Escherichia coli Proteins - isolation & purification ; Poly U - chemistry ; Poly(C) ; Poly(U) ; Protein S1 ; Ribosomal Proteins - isolation & purification ; Ribosomes - chemistry ; Sucrose - analogs & derivatives ; Sucrose - chemistry</subject><ispartof>Analytical biochemistry, 2017-01, Vol.517, p.53-55</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-469ac93227291c9799ee4a49bfbdaacf91480180eee17fe61c446787af1cdde33</citedby><cites>FETCH-LOGICAL-c350t-469ac93227291c9799ee4a49bfbdaacf91480180eee17fe61c446787af1cdde33</cites><orcidid>0000-0002-1829-5076</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2016.11.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27865825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baranovskaya, Marianna D.</creatorcontrib><creatorcontrib>Ugarov, Victor I.</creatorcontrib><creatorcontrib>Chetverina, Helena V.</creatorcontrib><creatorcontrib>Chetverin, Alexander B.</creatorcontrib><title>Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-bound polypyrimidine with the ribosomes, its length should not exceed several hundred nucleotides. Unlike popular affinity chromatography through a poly(U) Sepharose or poly(U) cellulose column, the method tolerates limited polyribonucleotide degradation by eventual traces of ribonucleases, and can readily be incorporated into standard protocols for the isolation of ribosomes by centrifugation.
•S1 is removed from ribosomes by centrifugation following incubation with poly(C) or poly(U).•Size matters: Optimally, the pyrimidine polyribonucleotide is 100–200 nt long.•The treatment is compatible with standard ribosome washing procedures.•The expensive affinity resin is no longer needed.</description><subject>Centrifugation</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Chromatography, Affinity</subject><subject>E. coli ribosomes</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli Proteins - isolation & purification</subject><subject>Poly U - chemistry</subject><subject>Poly(C)</subject><subject>Poly(U)</subject><subject>Protein S1</subject><subject>Ribosomal Proteins - isolation & purification</subject><subject>Ribosomes - chemistry</subject><subject>Sucrose - analogs & derivatives</subject><subject>Sucrose - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtu3DAQRYkgRrx20qcKWKaRPKMHJaYLjPUDMGDAj5qhqGHEhbTckJSN_ftosU46VzPFPRe4h7GvCDkCiotNrru8WL4cMQeED2yFIEUGJciPbAUAZVYI2Zyysxg3AIhVLT6x06JpRd0W9Yr9eqDJv-iRe8t3wSdyW_6I3AY_8XU0AwVnBqe58aPjwXU--okif3Vp8HPiaSA-RzrQ2lq3dWnPzbDAOvnfQe-G_Wd2YvUY6cvbPWfPV-uny5vs7v769vLnXWbKGlJWCamNLIuiKSQa2UhJVOlKdrbrtTZWYtUCtkBE2FgSaKpKNG2jLZq-p7I8Z9-PvcuKPzPFpCYXDY2j3pKfo8K2KuoaUYglCseoCT7GQFbtgpt02CsEdfCqNkp36uBVIarF64J8e2ufu4n6_8A_kUvgxzFAy8YXR0FF42hrqHeBTFK9d--3_wXXZoh7</recordid><startdate>20170115</startdate><enddate>20170115</enddate><creator>Baranovskaya, Marianna D.</creator><creator>Ugarov, Victor I.</creator><creator>Chetverina, Helena V.</creator><creator>Chetverin, Alexander B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1829-5076</orcidid></search><sort><creationdate>20170115</creationdate><title>Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography</title><author>Baranovskaya, Marianna D. ; Ugarov, Victor I. ; Chetverina, Helena V. ; Chetverin, Alexander B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-469ac93227291c9799ee4a49bfbdaacf91480180eee17fe61c446787af1cdde33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Centrifugation</topic><topic>Centrifugation, Density Gradient - methods</topic><topic>Chromatography, Affinity</topic><topic>E. coli ribosomes</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli Proteins - isolation & purification</topic><topic>Poly U - chemistry</topic><topic>Poly(C)</topic><topic>Poly(U)</topic><topic>Protein S1</topic><topic>Ribosomal Proteins - isolation & purification</topic><topic>Ribosomes - chemistry</topic><topic>Sucrose - analogs & derivatives</topic><topic>Sucrose - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baranovskaya, Marianna D.</creatorcontrib><creatorcontrib>Ugarov, Victor I.</creatorcontrib><creatorcontrib>Chetverina, Helena V.</creatorcontrib><creatorcontrib>Chetverin, Alexander B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baranovskaya, Marianna D.</au><au>Ugarov, Victor I.</au><au>Chetverina, Helena V.</au><au>Chetverin, Alexander B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2017-01-15</date><risdate>2017</risdate><volume>517</volume><spage>53</spage><epage>55</epage><pages>53-55</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-bound polypyrimidine with the ribosomes, its length should not exceed several hundred nucleotides. Unlike popular affinity chromatography through a poly(U) Sepharose or poly(U) cellulose column, the method tolerates limited polyribonucleotide degradation by eventual traces of ribonucleases, and can readily be incorporated into standard protocols for the isolation of ribosomes by centrifugation.
•S1 is removed from ribosomes by centrifugation following incubation with poly(C) or poly(U).•Size matters: Optimally, the pyrimidine polyribonucleotide is 100–200 nt long.•The treatment is compatible with standard ribosome washing procedures.•The expensive affinity resin is no longer needed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27865825</pmid><doi>10.1016/j.ab.2016.11.010</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0002-1829-5076</orcidid></addata></record> |
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| subjects | Centrifugation Centrifugation, Density Gradient - methods Chromatography, Affinity E. coli ribosomes Escherichia coli - chemistry Escherichia coli Proteins - isolation & purification Poly U - chemistry Poly(C) Poly(U) Protein S1 Ribosomal Proteins - isolation & purification Ribosomes - chemistry Sucrose - analogs & derivatives Sucrose - chemistry |
| title | Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography |
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