An efficient clean-up method for the GC–MS determination of methylsulfonyl-PCBs/DDEs extracted from various marine mammal tissues
Existing methods for analyzing methylsulfonyl-PCBs in animal tissues require multiple laborious clean-up steps prior to quantitation. In this paper, we report a new, efficient method for the isolation and determination of methylsulfonyl-PCB metabolites in the blubber, liver, kidney, lung, and milk f...
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| Veröffentlicht in: | Marine environmental research 2001-08, Vol.52 (2), p.127-150 |
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| creator | Herman, D.P Effler, J.I Boyd, D.T Krahn, M.M |
| description | Existing methods for analyzing methylsulfonyl-PCBs in animal tissues require multiple laborious clean-up steps prior to quantitation. In this paper, we report a new, efficient method for the isolation and determination of methylsulfonyl-PCB metabolites in the blubber, liver, kidney, lung, and milk from mammals of marine origin using: solvent extraction, GPC fractionation, and clean-up on an adsorption chromatography column prior to analysis by gas chromatography–mass spectrometry. The method is rapid and can be completed in about half the analysis time required by most other reported methods. The method exhibits excellent analyte recoveries (89–95%), and good reproducibilities with CV's ranging from 3–12% depending on analyte concentration. Detection limits are approximately 1 ng/g lipid. The method is further validated by comparing its quantitative results to that of an independent reference analytical method for several different marine mammal blubber samples. Finally, the method is applied to profile the distribution of methylsulfones among the various tissue types studied for a single bottle-nose dolphin (
Tursiops truncatus) sample. In addition, the extent of their transplacental transfer from mother to unborn fetus for several pilot whale (
Globicephala melaena) mother-fetus pairs is examined. |
| doi_str_mv | 10.1016/S0141-1136(00)00267-1 |
| format | Article |
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Tursiops truncatus) sample. In addition, the extent of their transplacental transfer from mother to unborn fetus for several pilot whale (
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Tursiops truncatus) sample. In addition, the extent of their transplacental transfer from mother to unborn fetus for several pilot whale (
Globicephala melaena) mother-fetus pairs is examined.</description><subject>Adipose Tissue - chemistry</subject><subject>Adsorption</subject><subject>Analytical technique</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Callorhinus ursinus</subject><subject>Chromatography - methods</subject><subject>Dichlorodiphenyl Dichloroethylene - analysis</subject><subject>Dichlorodiphenyl Dichloroethylene - pharmacokinetics</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Environmental Monitoring - methods</subject><subject>Environmental Pollutants - analysis</subject><subject>Environmental Pollutants - pharmacokinetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Globicephala melaena</topic><topic>Insecticides - analysis</topic><topic>Insecticides - pharmacokinetics</topic><topic>Male</topic><topic>Mammalia</topic><topic>Mammals</topic><topic>Marine</topic><topic>Marine mammal</topic><topic>mass spectroscopy</topic><topic>Maternal-Fetal Exchange</topic><topic>Methylsulfones</topic><topic>Organochlorine</topic><topic>PCB metabolites</topic><topic>Phocoena phocoena</topic><topic>Polychlorinated Biphenyls - analysis</topic><topic>Polychlorinated Biphenyls - pharmacokinetics</topic><topic>Pregnancy</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Tissue Distribution</topic><topic>Tursiops truncatus</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - pharmacokinetics</topic><topic>Zalophus californianus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herman, D.P</creatorcontrib><creatorcontrib>Effler, J.I</creatorcontrib><creatorcontrib>Boyd, D.T</creatorcontrib><creatorcontrib>Krahn, M.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herman, D.P</au><au>Effler, J.I</au><au>Boyd, D.T</au><au>Krahn, M.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An efficient clean-up method for the GC–MS determination of methylsulfonyl-PCBs/DDEs extracted from various marine mammal tissues</atitle><jtitle>Marine environmental research</jtitle><addtitle>Mar Environ Res</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>52</volume><issue>2</issue><spage>127</spage><epage>150</epage><pages>127-150</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>Existing methods for analyzing methylsulfonyl-PCBs in animal tissues require multiple laborious clean-up steps prior to quantitation. In this paper, we report a new, efficient method for the isolation and determination of methylsulfonyl-PCB metabolites in the blubber, liver, kidney, lung, and milk from mammals of marine origin using: solvent extraction, GPC fractionation, and clean-up on an adsorption chromatography column prior to analysis by gas chromatography–mass spectrometry. The method is rapid and can be completed in about half the analysis time required by most other reported methods. The method exhibits excellent analyte recoveries (89–95%), and good reproducibilities with CV's ranging from 3–12% depending on analyte concentration. Detection limits are approximately 1 ng/g lipid. The method is further validated by comparing its quantitative results to that of an independent reference analytical method for several different marine mammal blubber samples. Finally, the method is applied to profile the distribution of methylsulfones among the various tissue types studied for a single bottle-nose dolphin (
Tursiops truncatus) sample. In addition, the extent of their transplacental transfer from mother to unborn fetus for several pilot whale (
Globicephala melaena) mother-fetus pairs is examined.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11525427</pmid><doi>10.1016/S0141-1136(00)00267-1</doi><tpages>24</tpages></addata></record> |
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| identifier | ISSN: 0141-1136 |
| ispartof | Marine environmental research, 2001-08, Vol.52 (2), p.127-150 |
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| language | eng |
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| subjects | Adipose Tissue - chemistry Adsorption Analytical technique Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Callorhinus ursinus Chromatography - methods Dichlorodiphenyl Dichloroethylene - analysis Dichlorodiphenyl Dichloroethylene - pharmacokinetics Ecotoxicology, biological effects of pollution Environmental Monitoring - methods Environmental Pollutants - analysis Environmental Pollutants - pharmacokinetics Female Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry Globicephala melaena Insecticides - analysis Insecticides - pharmacokinetics Male Mammalia Mammals Marine Marine mammal mass spectroscopy Maternal-Fetal Exchange Methylsulfones Organochlorine PCB metabolites Phocoena phocoena Polychlorinated Biphenyls - analysis Polychlorinated Biphenyls - pharmacokinetics Pregnancy Sensitivity and Specificity Techniques Tissue Distribution Tursiops truncatus Water Pollutants, Chemical - analysis Water Pollutants, Chemical - pharmacokinetics Zalophus californianus |
| title | An efficient clean-up method for the GC–MS determination of methylsulfonyl-PCBs/DDEs extracted from various marine mammal tissues |
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