Microfluidic Tissue Mesodissection in Molecular Cancer Diagnostics
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In...
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| Veröffentlicht in: | SLAS technology 2017-08, Vol.22 (4), p.425-430 |
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| container_title | SLAS technology |
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| creator | Surrette, Christine Shoudy, David Corwin, Alex Gao, Wei Zavodszky, Maria I. Karsten, Stanislav L. Miller, Todd Gerdes, Michael J. Wood, Nichole Nelson, John R. Puleo, Chris M. |
| description | We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive. We demonstrate a microfluidic tool for rapid extraction of histological regions of interest from formalin-fixed paraffin-embedded tissue, which uses a simple and automated method that is compatible with most downstream enzymatic reactions, including protocols used for next-generation DNA sequencing. |
| doi_str_mv | 10.1177/2211068216680208 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 2472-6303 |
| ispartof | SLAS technology, 2017-08, Vol.22 (4), p.425-430 |
| issn | 2472-6303 2472-6311 |
| language | eng |
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| source | Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | and genomics clinical automation microfluidics molecular biology robotics and instrumentation |
| title | Microfluidic Tissue Mesodissection in Molecular Cancer Diagnostics |
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