The expression of bitter taste receptors in mesenteric, cerebral and omental arteries
Bitter taste is sensed by the bitter taste receptor (TAS2R), which is mainly expressed in the tongue as well as in extra-oral organs, such as the gastrointestinal tract, respiratory tract, brain, heart and testis. This study aimed to investigate whether TAS2R is expressed in the mesenteric, cerebral...
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| creator | Chen, Jing-Guo Ping, Na-Na Liang, Dong Li, Meng-Yi Mi, Yan-Ni Li, Sen Cao, Lei Cai, Yan Cao, Yong-Xiao |
| description | Bitter taste is sensed by the bitter taste receptor (TAS2R), which is mainly expressed in the tongue as well as in extra-oral organs, such as the gastrointestinal tract, respiratory tract, brain, heart and testis. This study aimed to investigate whether TAS2R is expressed in the mesenteric, cerebral and omental arteries.
The expression levels of TAS2R mRNA and protein were determined by reverse-transcription polymerase chain reaction and Western blotting, respectively. The location of TAS2R was determined by immunofluorescence imaging. TAS2R agonists were used in a sensitive myograph to study the function of TAS2R in arteries.
The mRNA of rat TAS2Rs, including rTAS2R39, rTAS2R40, rTAS2R108, rTAS2R114, rTAS2R130, rTAS2R137, and rTAS2R140, was expressed in rat mesenteric and cerebral arteries, but rTAS2R114 was not expressed in the cerebral arteries. The mRNA of human TAS2Rs, including hTAS2R3, hTAS2R4, hTAS2R7, hTAS2R10, hTAS2R14, hTAS2R39 and hTAS2R40, was expressed in human omental arteries. The TAS2R7 protein was expressed in rat mesenteric and cerebral arteries, as well as in human omental arteries. Immunofluorescence imaging confirmed that TAS2R7 was located in vascular smooth muscle cells and endothelial cells. The TAS2R agonists, chloroquine and quinine relaxed rat mesenteric arteries and cerebral arteries and human omental arteries in a concentration-dependent manner.
TAS2R is expressed in the arteries of systemic circulation, including rat mesenteric and cerebral arteries and human omental arteries. This study provides evidence that TAS2R do exist in the arteries and may be involved in the mediation of vessel functions. |
| doi_str_mv | 10.1016/j.lfs.2016.11.010 |
| format | Article |
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The expression levels of TAS2R mRNA and protein were determined by reverse-transcription polymerase chain reaction and Western blotting, respectively. The location of TAS2R was determined by immunofluorescence imaging. TAS2R agonists were used in a sensitive myograph to study the function of TAS2R in arteries.
The mRNA of rat TAS2Rs, including rTAS2R39, rTAS2R40, rTAS2R108, rTAS2R114, rTAS2R130, rTAS2R137, and rTAS2R140, was expressed in rat mesenteric and cerebral arteries, but rTAS2R114 was not expressed in the cerebral arteries. The mRNA of human TAS2Rs, including hTAS2R3, hTAS2R4, hTAS2R7, hTAS2R10, hTAS2R14, hTAS2R39 and hTAS2R40, was expressed in human omental arteries. The TAS2R7 protein was expressed in rat mesenteric and cerebral arteries, as well as in human omental arteries. Immunofluorescence imaging confirmed that TAS2R7 was located in vascular smooth muscle cells and endothelial cells. The TAS2R agonists, chloroquine and quinine relaxed rat mesenteric arteries and cerebral arteries and human omental arteries in a concentration-dependent manner.
TAS2R is expressed in the arteries of systemic circulation, including rat mesenteric and cerebral arteries and human omental arteries. This study provides evidence that TAS2R do exist in the arteries and may be involved in the mediation of vessel functions.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2016.11.010</identifier><identifier>PMID: 27863958</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Arteries ; Bitter taste ; Bitter taste receptor ; Brain ; Brain research ; Cerebral Arteries - metabolism ; Cerebral artery ; Cerebral blood flow ; Cerebrovascular Circulation ; Chloroquine ; Chloroquine - chemistry ; Circulation ; Endothelial cells ; Female ; Gastrointestinal tract ; Gene expression ; Gene Expression Regulation ; Humans ; Immunofluorescence ; Liver - metabolism ; Male ; Mesenteric artery ; Mesentery - blood supply ; Microscopy, Fluorescence ; Middle Aged ; Neuroimaging ; Omental artery ; Omentum - blood supply ; Omentum - metabolism ; Organs ; Polymerase chain reaction ; Quinine ; Quinine - chemistry ; Rats ; Rats, Sprague-Dawley ; Receptors ; Receptors, G-Protein-Coupled - metabolism ; Respiratory tract ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Smooth muscle ; Taste ; Taste receptors ; Tongue ; Tongue - metabolism ; Vasodilation ; Veins & arteries ; Western blotting</subject><ispartof>Life sciences (1973), 2017-02, Vol.170, p.16-24</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Feb 1, 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-858c19328757aacf080710ce5456f50912963941c5740b88238401e3f221b2a93</citedby><cites>FETCH-LOGICAL-c381t-858c19328757aacf080710ce5456f50912963941c5740b88238401e3f221b2a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320516306518$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27863958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jing-Guo</creatorcontrib><creatorcontrib>Ping, Na-Na</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><creatorcontrib>Li, Meng-Yi</creatorcontrib><creatorcontrib>Mi, Yan-Ni</creatorcontrib><creatorcontrib>Li, Sen</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Cai, Yan</creatorcontrib><creatorcontrib>Cao, Yong-Xiao</creatorcontrib><title>The expression of bitter taste receptors in mesenteric, cerebral and omental arteries</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Bitter taste is sensed by the bitter taste receptor (TAS2R), which is mainly expressed in the tongue as well as in extra-oral organs, such as the gastrointestinal tract, respiratory tract, brain, heart and testis. This study aimed to investigate whether TAS2R is expressed in the mesenteric, cerebral and omental arteries.
The expression levels of TAS2R mRNA and protein were determined by reverse-transcription polymerase chain reaction and Western blotting, respectively. The location of TAS2R was determined by immunofluorescence imaging. TAS2R agonists were used in a sensitive myograph to study the function of TAS2R in arteries.
The mRNA of rat TAS2Rs, including rTAS2R39, rTAS2R40, rTAS2R108, rTAS2R114, rTAS2R130, rTAS2R137, and rTAS2R140, was expressed in rat mesenteric and cerebral arteries, but rTAS2R114 was not expressed in the cerebral arteries. The mRNA of human TAS2Rs, including hTAS2R3, hTAS2R4, hTAS2R7, hTAS2R10, hTAS2R14, hTAS2R39 and hTAS2R40, was expressed in human omental arteries. The TAS2R7 protein was expressed in rat mesenteric and cerebral arteries, as well as in human omental arteries. Immunofluorescence imaging confirmed that TAS2R7 was located in vascular smooth muscle cells and endothelial cells. The TAS2R agonists, chloroquine and quinine relaxed rat mesenteric arteries and cerebral arteries and human omental arteries in a concentration-dependent manner.
TAS2R is expressed in the arteries of systemic circulation, including rat mesenteric and cerebral arteries and human omental arteries. This study provides evidence that TAS2R do exist in the arteries and may be involved in the mediation of vessel functions.</description><subject>Animals</subject><subject>Arteries</subject><subject>Bitter taste</subject><subject>Bitter taste receptor</subject><subject>Brain</subject><subject>Brain research</subject><subject>Cerebral Arteries - metabolism</subject><subject>Cerebral artery</subject><subject>Cerebral blood flow</subject><subject>Cerebrovascular Circulation</subject><subject>Chloroquine</subject><subject>Chloroquine - chemistry</subject><subject>Circulation</subject><subject>Endothelial cells</subject><subject>Female</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mesenteric artery</subject><subject>Mesentery - blood supply</subject><subject>Microscopy, Fluorescence</subject><subject>Middle Aged</subject><subject>Neuroimaging</subject><subject>Omental artery</subject><subject>Omentum - blood supply</subject><subject>Omentum - metabolism</subject><subject>Organs</subject><subject>Polymerase chain reaction</subject><subject>Quinine</subject><subject>Quinine - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Respiratory tract</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Smooth muscle</subject><subject>Taste</subject><subject>Taste receptors</subject><subject>Tongue</subject><subject>Tongue - metabolism</subject><subject>Vasodilation</subject><subject>Veins & arteries</subject><subject>Western blotting</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqHDEMhk1JaTZpH6CXYsilh8xUssdrm5xCaJNCoJfkbDxeDfUyM97asyV9-3rZtIcccpKQPv2Ij7GPCC0Crr9s23Eorahti9gCwhu2QqNtA2uJJ2wFILpGClCn7KyULQAopeU7diq0WUurzIo9PvwkTk-7TKXENPM08D4uC2W--LIQzxRot6RceJz5RIXmuovhkgfK1Gc_cj9veJrq_NDnw5bKe_Z28GOhD8_1nD1--_pwc9fc_7j9fnN93wRpcGmMMgGtFEYr7X0YwIBGCKQ6tR4UWBS2vtlhULqD3hghTQdIchACe-GtPGefj7m7nH7tqSxuiiXQOPqZ0r44NB1qK63QFb14gW7TPs_1O4e2AwXVm6wUHqmQUymZBrfLcfL5j0NwB-du66pzd3DuEF11Xm8-PSfv-4k2_y_-Sa7A1RGgquJ3pOxKiDQH2sSqd3GbFF-J_wvn-49-</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Chen, Jing-Guo</creator><creator>Ping, Na-Na</creator><creator>Liang, Dong</creator><creator>Li, Meng-Yi</creator><creator>Mi, Yan-Ni</creator><creator>Li, Sen</creator><creator>Cao, Lei</creator><creator>Cai, Yan</creator><creator>Cao, Yong-Xiao</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>The expression of bitter taste receptors in mesenteric, cerebral and omental arteries</title><author>Chen, Jing-Guo ; Ping, Na-Na ; Liang, Dong ; Li, Meng-Yi ; Mi, Yan-Ni ; Li, Sen ; Cao, Lei ; Cai, Yan ; Cao, Yong-Xiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-858c19328757aacf080710ce5456f50912963941c5740b88238401e3f221b2a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Arteries</topic><topic>Bitter taste</topic><topic>Bitter taste receptor</topic><topic>Brain</topic><topic>Brain research</topic><topic>Cerebral Arteries - metabolism</topic><topic>Cerebral artery</topic><topic>Cerebral blood flow</topic><topic>Cerebrovascular Circulation</topic><topic>Chloroquine</topic><topic>Chloroquine - chemistry</topic><topic>Circulation</topic><topic>Endothelial cells</topic><topic>Female</topic><topic>Gastrointestinal tract</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mesenteric artery</topic><topic>Mesentery - blood supply</topic><topic>Microscopy, Fluorescence</topic><topic>Middle Aged</topic><topic>Neuroimaging</topic><topic>Omental artery</topic><topic>Omentum - blood supply</topic><topic>Omentum - metabolism</topic><topic>Organs</topic><topic>Polymerase chain reaction</topic><topic>Quinine</topic><topic>Quinine - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Respiratory tract</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Smooth muscle</topic><topic>Taste</topic><topic>Taste receptors</topic><topic>Tongue</topic><topic>Tongue - metabolism</topic><topic>Vasodilation</topic><topic>Veins & arteries</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jing-Guo</creatorcontrib><creatorcontrib>Ping, Na-Na</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><creatorcontrib>Li, Meng-Yi</creatorcontrib><creatorcontrib>Mi, Yan-Ni</creatorcontrib><creatorcontrib>Li, Sen</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Cai, Yan</creatorcontrib><creatorcontrib>Cao, Yong-Xiao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jing-Guo</au><au>Ping, Na-Na</au><au>Liang, Dong</au><au>Li, Meng-Yi</au><au>Mi, Yan-Ni</au><au>Li, Sen</au><au>Cao, Lei</au><au>Cai, Yan</au><au>Cao, Yong-Xiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The expression of bitter taste receptors in mesenteric, cerebral and omental arteries</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>170</volume><spage>16</spage><epage>24</epage><pages>16-24</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Bitter taste is sensed by the bitter taste receptor (TAS2R), which is mainly expressed in the tongue as well as in extra-oral organs, such as the gastrointestinal tract, respiratory tract, brain, heart and testis. This study aimed to investigate whether TAS2R is expressed in the mesenteric, cerebral and omental arteries.
The expression levels of TAS2R mRNA and protein were determined by reverse-transcription polymerase chain reaction and Western blotting, respectively. The location of TAS2R was determined by immunofluorescence imaging. TAS2R agonists were used in a sensitive myograph to study the function of TAS2R in arteries.
The mRNA of rat TAS2Rs, including rTAS2R39, rTAS2R40, rTAS2R108, rTAS2R114, rTAS2R130, rTAS2R137, and rTAS2R140, was expressed in rat mesenteric and cerebral arteries, but rTAS2R114 was not expressed in the cerebral arteries. The mRNA of human TAS2Rs, including hTAS2R3, hTAS2R4, hTAS2R7, hTAS2R10, hTAS2R14, hTAS2R39 and hTAS2R40, was expressed in human omental arteries. The TAS2R7 protein was expressed in rat mesenteric and cerebral arteries, as well as in human omental arteries. Immunofluorescence imaging confirmed that TAS2R7 was located in vascular smooth muscle cells and endothelial cells. The TAS2R agonists, chloroquine and quinine relaxed rat mesenteric arteries and cerebral arteries and human omental arteries in a concentration-dependent manner.
TAS2R is expressed in the arteries of systemic circulation, including rat mesenteric and cerebral arteries and human omental arteries. This study provides evidence that TAS2R do exist in the arteries and may be involved in the mediation of vessel functions.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>27863958</pmid><doi>10.1016/j.lfs.2016.11.010</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arteries Bitter taste Bitter taste receptor Brain Brain research Cerebral Arteries - metabolism Cerebral artery Cerebral blood flow Cerebrovascular Circulation Chloroquine Chloroquine - chemistry Circulation Endothelial cells Female Gastrointestinal tract Gene expression Gene Expression Regulation Humans Immunofluorescence Liver - metabolism Male Mesenteric artery Mesentery - blood supply Microscopy, Fluorescence Middle Aged Neuroimaging Omental artery Omentum - blood supply Omentum - metabolism Organs Polymerase chain reaction Quinine Quinine - chemistry Rats Rats, Sprague-Dawley Receptors Receptors, G-Protein-Coupled - metabolism Respiratory tract Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Smooth muscle Taste Taste receptors Tongue Tongue - metabolism Vasodilation Veins & arteries Western blotting |
| title | The expression of bitter taste receptors in mesenteric, cerebral and omental arteries |
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