MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake
Summary The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of the mntABC and mntH genes, which encode putative manganese transporters. Mutation of mntABC produced a growth defect in metal‐depleted medium and increased sensitivity to intracellularly generated superoxide radicals....
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| Veröffentlicht in: | Molecular microbiology 2002-06, Vol.44 (5), p.1269-1286 |
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| container_title | Molecular microbiology |
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| creator | Horsburgh, Malcolm J. Wharton, Stephen J. Cox, Alan G. Ingham, Eileen Peacock, Sharon Foster, Simon J. |
| description | Summary
The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of
the mntABC and mntH genes, which encode putative manganese transporters.
Mutation of mntABC produced a growth defect in metal‐depleted medium and increased
sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted
from diminished uptake of manganese and were rescued by the addition of excess Mn(II).
Resistance to superoxide was incompletely rescued by Mn(II) for STE035 (mntA mntH),
and the strain had reduced virulence in a murine abscess model of infection. Expression
of mntABC was repressed by Mn(II) in an MntR‐dependent manner, which contrasts
with the expression of mntH that was not repressed in elevated Mn(II) and
was decreased in an mntR mutant. This demonstrates that MntR acts as a negative
and positive regulator of these loci re‐spectively. PerR, the peroxide resistance
regulon repressor, acts with MntR to control the expression of mntABC and
manganese uptake. The expression of the PerR‐regulated genes, katA (catalase),
ftn (ferritin) and fur (ferric uptake regulator), was diminished in
STE031 (mntR) when grown in excess Mn(II). Therefore, the control of Mn(II)‐regulated
members of the PerR regulon and the Fur protein is modulated by MntR through its
control of Mn(II) uptake. The co‐ordinated regulation of metal ion homeostasis and
oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed. |
| doi_str_mv | 10.1046/j.1365-2958.2002.02944.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18411213</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18411213</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4734-1319855609d23bce938bed84c1b5c8485ffd757a4a903b9d01a75d905c5c6fbe3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EotvCX0AWB24J_kzsAwdUFVqpq1YtSNwsx5nsZkniYMdi984PJ2FXVOLEaUYzz7wa6UEIU5JTIor3u5zyQmZMS5UzQlhOmBYi3z9Dq7-L52hFtCQZV-zbGTqPcUcI5aTgL9EZZYQpXuoV-rUepgfc-zp1doKIYT8GiLH1A_YNnraA7yE84ACb1M0zO9Q4phGC37c1zOPYxskODnA74MfJjttD5513LkVsU4C5TNvg02aLnR-m4LsltrfDxg4QAadxst_hFXrR2C7C61O9QF8_XX25vM5u7z7fXH68zZwoucgop1pJWRBdM1450FxVUCvhaCWdEko2TV3K0gqrCa90TagtZa2JdNIVTQX8Ar075o7B_0gQJ9O30UHXzc_4FA1VglJG-Qy-_Qfc-RSG-TdDdSEZo2KB1BFywccYoDFjaHsbDoYSs2gyO7PYMIsNs2gyfzSZ_Xz65pSfqh7qp8OTlxn4cAR-th0c_jvYrNc3S8d_A5Bao4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>196522143</pqid></control><display><type>article</type><title>MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Horsburgh, Malcolm J. ; Wharton, Stephen J. ; Cox, Alan G. ; Ingham, Eileen ; Peacock, Sharon ; Foster, Simon J.</creator><creatorcontrib>Horsburgh, Malcolm J. ; Wharton, Stephen J. ; Cox, Alan G. ; Ingham, Eileen ; Peacock, Sharon ; Foster, Simon J.</creatorcontrib><description>Summary
The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of
the mntABC and mntH genes, which encode putative manganese transporters.
Mutation of mntABC produced a growth defect in metal‐depleted medium and increased
sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted
from diminished uptake of manganese and were rescued by the addition of excess Mn(II).
Resistance to superoxide was incompletely rescued by Mn(II) for STE035 (mntA mntH),
and the strain had reduced virulence in a murine abscess model of infection. Expression
of mntABC was repressed by Mn(II) in an MntR‐dependent manner, which contrasts
with the expression of mntH that was not repressed in elevated Mn(II) and
was decreased in an mntR mutant. This demonstrates that MntR acts as a negative
and positive regulator of these loci re‐spectively. PerR, the peroxide resistance
regulon repressor, acts with MntR to control the expression of mntABC and
manganese uptake. The expression of the PerR‐regulated genes, katA (catalase),
ftn (ferritin) and fur (ferric uptake regulator), was diminished in
STE031 (mntR) when grown in excess Mn(II). Therefore, the control of Mn(II)‐regulated
members of the PerR regulon and the Fur protein is modulated by MntR through its
control of Mn(II) uptake. The co‐ordinated regulation of metal ion homeostasis and
oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2002.02944.x</identifier><identifier>PMID: 12028379</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Endothelium, Vascular - microbiology ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genes, Reporter ; Herbicides - pharmacology ; Homeostasis ; Humans ; Manganese - metabolism ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Paraquat - pharmacology ; Promoter Regions, Genetic ; Recombinant Fusion Proteins ; Regulon - genetics ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - metabolism ; Staphylococcus aureus - pathogenicity ; Superoxides - metabolism ; Transcription Factors</subject><ispartof>Molecular microbiology, 2002-06, Vol.44 (5), p.1269-1286</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Jun 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4734-1319855609d23bce938bed84c1b5c8485ffd757a4a903b9d01a75d905c5c6fbe3</citedby><cites>FETCH-LOGICAL-c4734-1319855609d23bce938bed84c1b5c8485ffd757a4a903b9d01a75d905c5c6fbe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2002.02944.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2002.02944.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27903,27904,45553,45554,46387,46811</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12028379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Horsburgh, Malcolm J.</creatorcontrib><creatorcontrib>Wharton, Stephen J.</creatorcontrib><creatorcontrib>Cox, Alan G.</creatorcontrib><creatorcontrib>Ingham, Eileen</creatorcontrib><creatorcontrib>Peacock, Sharon</creatorcontrib><creatorcontrib>Foster, Simon J.</creatorcontrib><title>MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of
the mntABC and mntH genes, which encode putative manganese transporters.
Mutation of mntABC produced a growth defect in metal‐depleted medium and increased
sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted
from diminished uptake of manganese and were rescued by the addition of excess Mn(II).
Resistance to superoxide was incompletely rescued by Mn(II) for STE035 (mntA mntH),
and the strain had reduced virulence in a murine abscess model of infection. Expression
of mntABC was repressed by Mn(II) in an MntR‐dependent manner, which contrasts
with the expression of mntH that was not repressed in elevated Mn(II) and
was decreased in an mntR mutant. This demonstrates that MntR acts as a negative
and positive regulator of these loci re‐spectively. PerR, the peroxide resistance
regulon repressor, acts with MntR to control the expression of mntABC and
manganese uptake. The expression of the PerR‐regulated genes, katA (catalase),
ftn (ferritin) and fur (ferric uptake regulator), was diminished in
STE031 (mntR) when grown in excess Mn(II). Therefore, the control of Mn(II)‐regulated
members of the PerR regulon and the Fur protein is modulated by MntR through its
control of Mn(II) uptake. The co‐ordinated regulation of metal ion homeostasis and
oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium, Vascular - microbiology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genes, Reporter</subject><subject>Herbicides - pharmacology</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Manganese - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Paraquat - pharmacology</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins</subject><subject>Regulon - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Superoxides - metabolism</subject><subject>Transcription Factors</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCX0AWB24J_kzsAwdUFVqpq1YtSNwsx5nsZkniYMdi984PJ2FXVOLEaUYzz7wa6UEIU5JTIor3u5zyQmZMS5UzQlhOmBYi3z9Dq7-L52hFtCQZV-zbGTqPcUcI5aTgL9EZZYQpXuoV-rUepgfc-zp1doKIYT8GiLH1A_YNnraA7yE84ACb1M0zO9Q4phGC37c1zOPYxskODnA74MfJjttD5513LkVsU4C5TNvg02aLnR-m4LsltrfDxg4QAadxst_hFXrR2C7C61O9QF8_XX25vM5u7z7fXH68zZwoucgop1pJWRBdM1450FxVUCvhaCWdEko2TV3K0gqrCa90TagtZa2JdNIVTQX8Ar075o7B_0gQJ9O30UHXzc_4FA1VglJG-Qy-_Qfc-RSG-TdDdSEZo2KB1BFywccYoDFjaHsbDoYSs2gyO7PYMIsNs2gyfzSZ_Xz65pSfqh7qp8OTlxn4cAR-th0c_jvYrNc3S8d_A5Bao4A</recordid><startdate>200206</startdate><enddate>200206</enddate><creator>Horsburgh, Malcolm J.</creator><creator>Wharton, Stephen J.</creator><creator>Cox, Alan G.</creator><creator>Ingham, Eileen</creator><creator>Peacock, Sharon</creator><creator>Foster, Simon J.</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200206</creationdate><title>MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake</title><author>Horsburgh, Malcolm J. ; Wharton, Stephen J. ; Cox, Alan G. ; Ingham, Eileen ; Peacock, Sharon ; Foster, Simon J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4734-1319855609d23bce938bed84c1b5c8485ffd757a4a903b9d01a75d905c5c6fbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium, Vascular - microbiology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genes, Reporter</topic><topic>Herbicides - pharmacology</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Manganese - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Paraquat - pharmacology</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins</topic><topic>Regulon - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Superoxides - metabolism</topic><topic>Transcription Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horsburgh, Malcolm J.</creatorcontrib><creatorcontrib>Wharton, Stephen J.</creatorcontrib><creatorcontrib>Cox, Alan G.</creatorcontrib><creatorcontrib>Ingham, Eileen</creatorcontrib><creatorcontrib>Peacock, Sharon</creatorcontrib><creatorcontrib>Foster, Simon J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horsburgh, Malcolm J.</au><au>Wharton, Stephen J.</au><au>Cox, Alan G.</au><au>Ingham, Eileen</au><au>Peacock, Sharon</au><au>Foster, Simon J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2002-06</date><risdate>2002</risdate><volume>44</volume><issue>5</issue><spage>1269</spage><epage>1286</epage><pages>1269-1286</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of
the mntABC and mntH genes, which encode putative manganese transporters.
Mutation of mntABC produced a growth defect in metal‐depleted medium and increased
sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted
from diminished uptake of manganese and were rescued by the addition of excess Mn(II).
Resistance to superoxide was incompletely rescued by Mn(II) for STE035 (mntA mntH),
and the strain had reduced virulence in a murine abscess model of infection. Expression
of mntABC was repressed by Mn(II) in an MntR‐dependent manner, which contrasts
with the expression of mntH that was not repressed in elevated Mn(II) and
was decreased in an mntR mutant. This demonstrates that MntR acts as a negative
and positive regulator of these loci re‐spectively. PerR, the peroxide resistance
regulon repressor, acts with MntR to control the expression of mntABC and
manganese uptake. The expression of the PerR‐regulated genes, katA (catalase),
ftn (ferritin) and fur (ferric uptake regulator), was diminished in
STE031 (mntR) when grown in excess Mn(II). Therefore, the control of Mn(II)‐regulated
members of the PerR regulon and the Fur protein is modulated by MntR through its
control of Mn(II) uptake. The co‐ordinated regulation of metal ion homeostasis and
oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12028379</pmid><doi>10.1046/j.1365-2958.2002.02944.x</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Endothelium, Vascular - microbiology Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Reporter Herbicides - pharmacology Homeostasis Humans Manganese - metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Paraquat - pharmacology Promoter Regions, Genetic Recombinant Fusion Proteins Regulon - genetics Repressor Proteins - genetics Repressor Proteins - metabolism Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - metabolism Staphylococcus aureus - pathogenicity Superoxides - metabolism Transcription Factors |
| title | MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake |
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