Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene
•S. sp. carrying of vgb was constructed to improve welan gum yield.•An increase of 34.6g/L welan gum was attained with VHb expression.•The rheological behavior of welan gum solutions had no change after vgb insertion.•VHb expression enhanced oxygen uptake rate and ATP level for welan gum production....
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| Veröffentlicht in: | Carbohydrate polymers 2017-01, Vol.156, p.135-142 |
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| creator | Liu, Xiaoliu Zhu, Ping Jiang, Ruifan Wu, Lingtian Feng, Xiaohai Li, Sha Xu, Hong |
| description | •S. sp. carrying of vgb was constructed to improve welan gum yield.•An increase of 34.6g/L welan gum was attained with VHb expression.•The rheological behavior of welan gum solutions had no change after vgb insertion.•VHb expression enhanced oxygen uptake rate and ATP level for welan gum production.
Welan gum is a microbial polysaccharide produced by Sphingomonas sp. Its production is limited by the dissolved oxygen levels in the highly viscous fermentation. A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in Sphingomonas sp. HT-1 was investigated to alleviate oxygen limitation and improve the yield of welan gum. Ultimately, the welan gum production increased from 25.3g/L to 34.6g/L, whereas the rheological behavior of welan gum solutions remained virtually unchanged. The transcriptional levels of the key genes in the electron transfer chain, TCA cycle and welan gum synthesis pathway, as well as ATP level revealed that the VHb expression in Sphingomonas sp. HT-1 enhanced welan gum biosynthesis by improving respiration and ATP supply. This study would pave the genetic manipulation way for enhancing welan gum yield, and it’s also of great importance for the industrial applications of welan gum under harsh conditions. |
| doi_str_mv | 10.1016/j.carbpol.2016.08.081 |
| format | Article |
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Welan gum is a microbial polysaccharide produced by Sphingomonas sp. Its production is limited by the dissolved oxygen levels in the highly viscous fermentation. A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in Sphingomonas sp. HT-1 was investigated to alleviate oxygen limitation and improve the yield of welan gum. Ultimately, the welan gum production increased from 25.3g/L to 34.6g/L, whereas the rheological behavior of welan gum solutions remained virtually unchanged. The transcriptional levels of the key genes in the electron transfer chain, TCA cycle and welan gum synthesis pathway, as well as ATP level revealed that the VHb expression in Sphingomonas sp. HT-1 enhanced welan gum biosynthesis by improving respiration and ATP supply. This study would pave the genetic manipulation way for enhancing welan gum yield, and it’s also of great importance for the industrial applications of welan gum under harsh conditions.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2016.08.081</identifier><identifier>PMID: 27842807</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Bacterial Proteins - genetics ; Fermentation ; Industrial Microbiology ; Microorganisms, Genetically-Modified - metabolism ; Polysaccharides, Bacterial - biosynthesis ; Rheological behavior ; Sphingomonas - genetics ; Sphingomonas - metabolism ; Truncated Hemoglobins - genetics ; Vitreoscilla hemoglobin gene ; Welan gum</subject><ispartof>Carbohydrate polymers, 2017-01, Vol.156, p.135-142</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-afd51f492fae1e6becae285cdd76e0b13c3704d9f897a355f91f600a8ca3ad8e3</citedby><cites>FETCH-LOGICAL-c468t-afd51f492fae1e6becae285cdd76e0b13c3704d9f897a355f91f600a8ca3ad8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2016.08.081$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27842807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiaoliu</creatorcontrib><creatorcontrib>Zhu, Ping</creatorcontrib><creatorcontrib>Jiang, Ruifan</creatorcontrib><creatorcontrib>Wu, Lingtian</creatorcontrib><creatorcontrib>Feng, Xiaohai</creatorcontrib><creatorcontrib>Li, Sha</creatorcontrib><creatorcontrib>Xu, Hong</creatorcontrib><title>Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•S. sp. carrying of vgb was constructed to improve welan gum yield.•An increase of 34.6g/L welan gum was attained with VHb expression.•The rheological behavior of welan gum solutions had no change after vgb insertion.•VHb expression enhanced oxygen uptake rate and ATP level for welan gum production.
Welan gum is a microbial polysaccharide produced by Sphingomonas sp. Its production is limited by the dissolved oxygen levels in the highly viscous fermentation. A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in Sphingomonas sp. HT-1 was investigated to alleviate oxygen limitation and improve the yield of welan gum. Ultimately, the welan gum production increased from 25.3g/L to 34.6g/L, whereas the rheological behavior of welan gum solutions remained virtually unchanged. The transcriptional levels of the key genes in the electron transfer chain, TCA cycle and welan gum synthesis pathway, as well as ATP level revealed that the VHb expression in Sphingomonas sp. HT-1 enhanced welan gum biosynthesis by improving respiration and ATP supply. This study would pave the genetic manipulation way for enhancing welan gum yield, and it’s also of great importance for the industrial applications of welan gum under harsh conditions.</description><subject>Bacterial Proteins - genetics</subject><subject>Fermentation</subject><subject>Industrial Microbiology</subject><subject>Microorganisms, Genetically-Modified - metabolism</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Rheological behavior</subject><subject>Sphingomonas - genetics</subject><subject>Sphingomonas - metabolism</subject><subject>Truncated Hemoglobins - genetics</subject><subject>Vitreoscilla hemoglobin gene</subject><subject>Welan gum</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v1DAQxS0EotuFjwDykUuCJ3-dE6qq0laqxIHC1XLscdarxA52si3fvl7t0ivWSCNL772Z-RHyCVgODJqv-1zJ0M9-zIv0zRlPBW_IBnjbZVBW1VuyYVBVGW-gvSCXMe5Zeg2w9-SiaHlVcNZuyPON20mncEK3UG_oE47S0WGd6By8XtVivaPW0Z_zzrrBT97JSOOc07vHDOjBSrrDBYMf_eDXSPF5Dhjj0ZTCftsloI_KjuNRN_lh9H0KG9DhB_LOyDHix3Pfkl_fbx6v77KHH7f311cPmaoavmTS6BpM1RVGImDTo5JY8Fpp3TbIeihV2bJKd4Z3rSzr2nRgGsYkV7KUmmO5JV9OuemePyvGRUw2KkwbOUwbC-Bl15a8Tn1L6pNUBR9jQCPmYCcZ_gpg4ghd7MUZujhCF4ynguT7fB6x9hPqV9c_yknw7STAdOjBYhCJCSbq2gZUi9De_mfEC0CrmKs</recordid><startdate>20170120</startdate><enddate>20170120</enddate><creator>Liu, Xiaoliu</creator><creator>Zhu, Ping</creator><creator>Jiang, Ruifan</creator><creator>Wu, Lingtian</creator><creator>Feng, Xiaohai</creator><creator>Li, Sha</creator><creator>Xu, Hong</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170120</creationdate><title>Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene</title><author>Liu, Xiaoliu ; Zhu, Ping ; Jiang, Ruifan ; Wu, Lingtian ; Feng, Xiaohai ; Li, Sha ; Xu, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-afd51f492fae1e6becae285cdd76e0b13c3704d9f897a355f91f600a8ca3ad8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Fermentation</topic><topic>Industrial Microbiology</topic><topic>Microorganisms, Genetically-Modified - metabolism</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Rheological behavior</topic><topic>Sphingomonas - genetics</topic><topic>Sphingomonas - metabolism</topic><topic>Truncated Hemoglobins - genetics</topic><topic>Vitreoscilla hemoglobin gene</topic><topic>Welan gum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiaoliu</creatorcontrib><creatorcontrib>Zhu, Ping</creatorcontrib><creatorcontrib>Jiang, Ruifan</creatorcontrib><creatorcontrib>Wu, Lingtian</creatorcontrib><creatorcontrib>Feng, Xiaohai</creatorcontrib><creatorcontrib>Li, Sha</creatorcontrib><creatorcontrib>Xu, Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiaoliu</au><au>Zhu, Ping</au><au>Jiang, Ruifan</au><au>Wu, Lingtian</au><au>Feng, Xiaohai</au><au>Li, Sha</au><au>Xu, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2017-01-20</date><risdate>2017</risdate><volume>156</volume><spage>135</spage><epage>142</epage><pages>135-142</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>•S. sp. carrying of vgb was constructed to improve welan gum yield.•An increase of 34.6g/L welan gum was attained with VHb expression.•The rheological behavior of welan gum solutions had no change after vgb insertion.•VHb expression enhanced oxygen uptake rate and ATP level for welan gum production.
Welan gum is a microbial polysaccharide produced by Sphingomonas sp. Its production is limited by the dissolved oxygen levels in the highly viscous fermentation. A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in Sphingomonas sp. HT-1 was investigated to alleviate oxygen limitation and improve the yield of welan gum. Ultimately, the welan gum production increased from 25.3g/L to 34.6g/L, whereas the rheological behavior of welan gum solutions remained virtually unchanged. The transcriptional levels of the key genes in the electron transfer chain, TCA cycle and welan gum synthesis pathway, as well as ATP level revealed that the VHb expression in Sphingomonas sp. HT-1 enhanced welan gum biosynthesis by improving respiration and ATP supply. This study would pave the genetic manipulation way for enhancing welan gum yield, and it’s also of great importance for the industrial applications of welan gum under harsh conditions.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27842807</pmid><doi>10.1016/j.carbpol.2016.08.081</doi><tpages>8</tpages></addata></record> |
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| subjects | Bacterial Proteins - genetics Fermentation Industrial Microbiology Microorganisms, Genetically-Modified - metabolism Polysaccharides, Bacterial - biosynthesis Rheological behavior Sphingomonas - genetics Sphingomonas - metabolism Truncated Hemoglobins - genetics Vitreoscilla hemoglobin gene Welan gum |
| title | Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene |
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