Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae
The antifungal effect of hevein, the chitin binding lectin from rubber plant ( Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by Alternaria brassicae. A cDNA encoding hevein was transferred into Indian mustard ( Brassic...
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| Veröffentlicht in: | Plant science (Limerick) 2002-03, Vol.162 (3), p.441-448 |
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| container_title | Plant science (Limerick) |
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| creator | Kanrar, Siddhartha Venkateswari, Jaganatha C Kirti, Pulugurtha B Chopra, Virendra L |
| description | The antifungal effect of hevein, the chitin binding lectin from rubber plant (
Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by
Alternaria brassicae. A cDNA encoding hevein was transferred into Indian mustard (
Brassica juncea cv. RLM-198). Southern analysis of the putative transgenics showed integration of the transgene. Northern and Western analyses proved that the integrated transgene is expressed in the transgenics. In whole plant bioassay under glasshouse conditions, transgenics were found to possess parameters that are associated with resistance such as longer incubation and latent period; smaller necrotic lesion size; lower disease intensity and delayed senescence. |
| doi_str_mv | 10.1016/S0168-9452(01)00588-X |
| format | Article |
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Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by
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Brassica juncea cv. RLM-198). Southern analysis of the putative transgenics showed integration of the transgene. Northern and Western analyses proved that the integrated transgene is expressed in the transgenics. In whole plant bioassay under glasshouse conditions, transgenics were found to possess parameters that are associated with resistance such as longer incubation and latent period; smaller necrotic lesion size; lower disease intensity and delayed senescence.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/S0168-9452(01)00588-X</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Alternaria blight ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Genetics and breeding of economic plants ; Hevein ; Indian mustard ; Pest resistance ; Plant pathogens ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Plant science (Limerick), 2002-03, Vol.162 (3), p.441-448</ispartof><rights>2002 Elsevier Science Ireland Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-4e2a76926291516565518f165d36949ff0716549074bf3905f1dcde6614a01c03</citedby><cites>FETCH-LOGICAL-c420t-4e2a76926291516565518f165d36949ff0716549074bf3905f1dcde6614a01c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-9452(01)00588-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13522211$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanrar, Siddhartha</creatorcontrib><creatorcontrib>Venkateswari, Jaganatha C</creatorcontrib><creatorcontrib>Kirti, Pulugurtha B</creatorcontrib><creatorcontrib>Chopra, Virendra L</creatorcontrib><title>Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae</title><title>Plant science (Limerick)</title><description>The antifungal effect of hevein, the chitin binding lectin from rubber plant (
Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by
Alternaria brassicae. A cDNA encoding hevein was transferred into Indian mustard (
Brassica juncea cv. RLM-198). Southern analysis of the putative transgenics showed integration of the transgene. Northern and Western analyses proved that the integrated transgene is expressed in the transgenics. In whole plant bioassay under glasshouse conditions, transgenics were found to possess parameters that are associated with resistance such as longer incubation and latent period; smaller necrotic lesion size; lower disease intensity and delayed senescence.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Alternaria blight</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics and breeding of economic plants</subject><subject>Hevein</subject><subject>Indian mustard</subject><subject>Pest resistance</subject><subject>Plant pathogens</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkE1rGzEQhkVpoG6an1DQpaWFbCJpV_txKsHkw2DIIQnkJmTtyFZZa50ZOTT99ZHtkB57kTTimXekh7GvUpxJIevzu7y0RVdp9UPIn0Loti0eP7CJbJuyUEp3H9nkHfnEPhP9FkIorZsJ-3uPNtISYnAc_mwQiMIY-ej5Cp4hxFOeVsBxu1gA8oQAfACXdvch8lnsg418vaVksedujB6Q-AbHtINyjl3aECnxiyEBRovB8gXaPMNZ-MKOvB0ITt72Y_ZwdXk_vSnmt9ez6cW8cJUSqahA2abuVK06qWWta61l6_OhL-uu6rwXTS6qTjTVwped0F72roe6lpUV0onymH0_5OZ3PW2BklkHcjAMNsK4JSPbMieXTQb1AXQ4EiF4s8GwtvhipDA702Zv2uw0GiHN3rR5zH3f3gZYcnbw2agL9K-51EopKTP368BB_u1zADTkAkQHfcDsy_Rj-M-kV7EAk14</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Kanrar, Siddhartha</creator><creator>Venkateswari, Jaganatha C</creator><creator>Kirti, Pulugurtha B</creator><creator>Chopra, Virendra L</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20020301</creationdate><title>Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae</title><author>Kanrar, Siddhartha ; Venkateswari, Jaganatha C ; Kirti, Pulugurtha B ; Chopra, Virendra L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-4e2a76926291516565518f165d36949ff0716549074bf3905f1dcde6614a01c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Alternaria blight</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics and breeding of economic plants</topic><topic>Hevein</topic><topic>Indian mustard</topic><topic>Pest resistance</topic><topic>Plant pathogens</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanrar, Siddhartha</creatorcontrib><creatorcontrib>Venkateswari, Jaganatha C</creatorcontrib><creatorcontrib>Kirti, Pulugurtha B</creatorcontrib><creatorcontrib>Chopra, Virendra L</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanrar, Siddhartha</au><au>Venkateswari, Jaganatha C</au><au>Kirti, Pulugurtha B</au><au>Chopra, Virendra L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae</atitle><jtitle>Plant science (Limerick)</jtitle><date>2002-03-01</date><risdate>2002</risdate><volume>162</volume><issue>3</issue><spage>441</spage><epage>448</epage><pages>441-448</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>The antifungal effect of hevein, the chitin binding lectin from rubber plant (
Hevea brasiliensis), has been analyzed in transgenic plants for potential control of Alternaria blight in Indian mustard caused by
Alternaria brassicae. A cDNA encoding hevein was transferred into Indian mustard (
Brassica juncea cv. RLM-198). Southern analysis of the putative transgenics showed integration of the transgene. Northern and Western analyses proved that the integrated transgene is expressed in the transgenics. In whole plant bioassay under glasshouse conditions, transgenics were found to possess parameters that are associated with resistance such as longer incubation and latent period; smaller necrotic lesion size; lower disease intensity and delayed senescence.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/S0168-9452(01)00588-X</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 2002-03, Vol.162 (3), p.441-448 |
| issn | 0168-9452 1873-2259 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18391537 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Agronomy. Soil science and plant productions Alternaria blight Biological and medical sciences Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Hevein Indian mustard Pest resistance Plant pathogens Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae |
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