CENP-I Is Essential for Centromere Function in Vertebrate Cells
We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine th...
Gespeichert in:
Veröffentlicht in: | Developmental cell 2002-04, Vol.2 (4), p.463-476 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 476 |
---|---|
container_issue | 4 |
container_start_page | 463 |
container_title | Developmental cell |
container_volume | 2 |
creator | Nishihashi, Ai Haraguchi, Tokuko Hiraoka, Yasushi Ikemura, Toshimichi Regnier, Vinciane Dodson, Helen Earnshaw, William C. Fukagawa, Tatsuo |
description | We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere. |
doi_str_mv | 10.1016/S1534-5807(02)00144-2 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18383507</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1534580702001442</els_id><sourcerecordid>18383507</sourcerecordid><originalsourceid>FETCH-LOGICAL-c505t-a9a251bc66647009a08f199632d8e3709f1b1a8589b1741d2c2744b09addbc433</originalsourceid><addsrcrecordid>eNqFkMtKxDAUhoMozjj6CEpXootqTto06WqQMqMDgwpetiFNTyHSacekFXx7Mxdx6eqcxfefy0fIOdAboJDdvgBP0phLKq4ou6YU0jRmB2QMUsgYOIfD0P8iI3Li_UeAMpD0mIwAckFlno3JtJg9PseLaOGjmffY9lY3Ud25qAi961boMJoPrelt10a2jd7R9Vg63WMgmsafkqNaNx7P9nVC3uaz1-IhXj7dL4q7ZWw45X2sc804lCbLslRQmmsqa8jzLGGVxETQvIYStOQyL0GkUDHDRJqWAayq0qRJMiGXu7lr130O6Hu1st6EC3SL3eAVyEQmnIoA8h1oXOe9w1qtnV1p962Aqo05tTWnNloUZWprTrGQu9gvGMoVVn-pvaoATHcAhje_LDrljcXWYGUdml5Vnf1nxQ_yuXr8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18383507</pqid></control><display><type>article</type><title>CENP-I Is Essential for Centromere Function in Vertebrate Cells</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Nishihashi, Ai ; Haraguchi, Tokuko ; Hiraoka, Yasushi ; Ikemura, Toshimichi ; Regnier, Vinciane ; Dodson, Helen ; Earnshaw, William C. ; Fukagawa, Tatsuo</creator><creatorcontrib>Nishihashi, Ai ; Haraguchi, Tokuko ; Hiraoka, Yasushi ; Ikemura, Toshimichi ; Regnier, Vinciane ; Dodson, Helen ; Earnshaw, William C. ; Fukagawa, Tatsuo</creatorcontrib><description>We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.</description><identifier>ISSN: 1534-5807</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/S1534-5807(02)00144-2</identifier><identifier>PMID: 11970896</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Autoantigens ; Cell Cycle Proteins ; Cell Death - physiology ; Cells, Cultured ; Centromere - genetics ; Centromere - metabolism ; Centromere Protein A ; Chickens ; Chromosomal Proteins, Non-Histone - analysis ; Chromosomal Proteins, Non-Histone - genetics ; Chromosomal Proteins, Non-Histone - metabolism ; Chromosome Aberrations ; Chromosome Segregation ; Fungal Proteins - genetics ; Metaphase - physiology ; Microtubules - metabolism ; Mitosis - physiology ; Molecular Sequence Data ; Mutagenesis - physiology ; Schizosaccharomyces - genetics ; Schizosaccharomyces pombe Proteins ; Vertebrates</subject><ispartof>Developmental cell, 2002-04, Vol.2 (4), p.463-476</ispartof><rights>2002 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-a9a251bc66647009a08f199632d8e3709f1b1a8589b1741d2c2744b09addbc433</citedby><cites>FETCH-LOGICAL-c505t-a9a251bc66647009a08f199632d8e3709f1b1a8589b1741d2c2744b09addbc433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1534580702001442$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11970896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishihashi, Ai</creatorcontrib><creatorcontrib>Haraguchi, Tokuko</creatorcontrib><creatorcontrib>Hiraoka, Yasushi</creatorcontrib><creatorcontrib>Ikemura, Toshimichi</creatorcontrib><creatorcontrib>Regnier, Vinciane</creatorcontrib><creatorcontrib>Dodson, Helen</creatorcontrib><creatorcontrib>Earnshaw, William C.</creatorcontrib><creatorcontrib>Fukagawa, Tatsuo</creatorcontrib><title>CENP-I Is Essential for Centromere Function in Vertebrate Cells</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.</description><subject>Animals</subject><subject>Autoantigens</subject><subject>Cell Cycle Proteins</subject><subject>Cell Death - physiology</subject><subject>Cells, Cultured</subject><subject>Centromere - genetics</subject><subject>Centromere - metabolism</subject><subject>Centromere Protein A</subject><subject>Chickens</subject><subject>Chromosomal Proteins, Non-Histone - analysis</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Chromosome Aberrations</subject><subject>Chromosome Segregation</subject><subject>Fungal Proteins - genetics</subject><subject>Metaphase - physiology</subject><subject>Microtubules - metabolism</subject><subject>Mitosis - physiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis - physiology</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>Vertebrates</subject><issn>1534-5807</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMozjj6CEpXootqTto06WqQMqMDgwpetiFNTyHSacekFXx7Mxdx6eqcxfefy0fIOdAboJDdvgBP0phLKq4ou6YU0jRmB2QMUsgYOIfD0P8iI3Li_UeAMpD0mIwAckFlno3JtJg9PseLaOGjmffY9lY3Ud25qAi961boMJoPrelt10a2jd7R9Vg63WMgmsafkqNaNx7P9nVC3uaz1-IhXj7dL4q7ZWw45X2sc804lCbLslRQmmsqa8jzLGGVxETQvIYStOQyL0GkUDHDRJqWAayq0qRJMiGXu7lr130O6Hu1st6EC3SL3eAVyEQmnIoA8h1oXOe9w1qtnV1p962Aqo05tTWnNloUZWprTrGQu9gvGMoVVn-pvaoATHcAhje_LDrljcXWYGUdml5Vnf1nxQ_yuXr8</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Nishihashi, Ai</creator><creator>Haraguchi, Tokuko</creator><creator>Hiraoka, Yasushi</creator><creator>Ikemura, Toshimichi</creator><creator>Regnier, Vinciane</creator><creator>Dodson, Helen</creator><creator>Earnshaw, William C.</creator><creator>Fukagawa, Tatsuo</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20020401</creationdate><title>CENP-I Is Essential for Centromere Function in Vertebrate Cells</title><author>Nishihashi, Ai ; Haraguchi, Tokuko ; Hiraoka, Yasushi ; Ikemura, Toshimichi ; Regnier, Vinciane ; Dodson, Helen ; Earnshaw, William C. ; Fukagawa, Tatsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-a9a251bc66647009a08f199632d8e3709f1b1a8589b1741d2c2744b09addbc433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Autoantigens</topic><topic>Cell Cycle Proteins</topic><topic>Cell Death - physiology</topic><topic>Cells, Cultured</topic><topic>Centromere - genetics</topic><topic>Centromere - metabolism</topic><topic>Centromere Protein A</topic><topic>Chickens</topic><topic>Chromosomal Proteins, Non-Histone - analysis</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Chromosome Aberrations</topic><topic>Chromosome Segregation</topic><topic>Fungal Proteins - genetics</topic><topic>Metaphase - physiology</topic><topic>Microtubules - metabolism</topic><topic>Mitosis - physiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis - physiology</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishihashi, Ai</creatorcontrib><creatorcontrib>Haraguchi, Tokuko</creatorcontrib><creatorcontrib>Hiraoka, Yasushi</creatorcontrib><creatorcontrib>Ikemura, Toshimichi</creatorcontrib><creatorcontrib>Regnier, Vinciane</creatorcontrib><creatorcontrib>Dodson, Helen</creatorcontrib><creatorcontrib>Earnshaw, William C.</creatorcontrib><creatorcontrib>Fukagawa, Tatsuo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Developmental cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishihashi, Ai</au><au>Haraguchi, Tokuko</au><au>Hiraoka, Yasushi</au><au>Ikemura, Toshimichi</au><au>Regnier, Vinciane</au><au>Dodson, Helen</au><au>Earnshaw, William C.</au><au>Fukagawa, Tatsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CENP-I Is Essential for Centromere Function in Vertebrate Cells</atitle><jtitle>Developmental cell</jtitle><addtitle>Dev Cell</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>2</volume><issue>4</issue><spage>463</spage><epage>476</epage><pages>463-476</pages><issn>1534-5807</issn><eissn>1878-1551</eissn><abstract>We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11970896</pmid><doi>10.1016/S1534-5807(02)00144-2</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1534-5807 |
ispartof | Developmental cell, 2002-04, Vol.2 (4), p.463-476 |
issn | 1534-5807 1878-1551 |
language | eng |
recordid | cdi_proquest_miscellaneous_18383507 |
source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | Animals Autoantigens Cell Cycle Proteins Cell Death - physiology Cells, Cultured Centromere - genetics Centromere - metabolism Centromere Protein A Chickens Chromosomal Proteins, Non-Histone - analysis Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosome Aberrations Chromosome Segregation Fungal Proteins - genetics Metaphase - physiology Microtubules - metabolism Mitosis - physiology Molecular Sequence Data Mutagenesis - physiology Schizosaccharomyces - genetics Schizosaccharomyces pombe Proteins Vertebrates |
title | CENP-I Is Essential for Centromere Function in Vertebrate Cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A23%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CENP-I%20Is%20Essential%20for%20Centromere%20Function%20in%20Vertebrate%20Cells&rft.jtitle=Developmental%20cell&rft.au=Nishihashi,%20Ai&rft.date=2002-04-01&rft.volume=2&rft.issue=4&rft.spage=463&rft.epage=476&rft.pages=463-476&rft.issn=1534-5807&rft.eissn=1878-1551&rft_id=info:doi/10.1016/S1534-5807(02)00144-2&rft_dat=%3Cproquest_cross%3E18383507%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18383507&rft_id=info:pmid/11970896&rft_els_id=S1534580702001442&rfr_iscdi=true |