Fast determination of myo-inositol in milk powder by ultra high performance liquid chromatography coupled to tandem mass spectrometry

► Development of a fast, simple and reliable chromatographic method. ► Full validation of the method, including uncertainty estimation. ► Application in routine analysis. A simple and fast method has been developed and validated for the determination of myo-inositol in milk powder samples, using sol...

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Veröffentlicht in:Food chemistry 2011-12, Vol.129 (3), p.1281-1286
Hauptverfasser: Flores, María Isabel Alarcón, Moreno, José Luis Fernández, Frenich, Antonia Garrido, Vidal, José Luis Martínez
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Sprache:eng
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Zusammenfassung:► Development of a fast, simple and reliable chromatographic method. ► Full validation of the method, including uncertainty estimation. ► Application in routine analysis. A simple and fast method has been developed and validated for the determination of myo-inositol in milk powder samples, using solid–liquid extraction with water (0.01% formic acid, v/v):methanol (1:1, v/v). The determination was carried out by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC−MS/MS) using an electrospray ionisation source (ESI) in positive mode. Chromatographic separation was carried out using as mobile phase water (0.01% formic acid, v/v) and methanol in gradient mode. Data acquisition under MS/MS was achieved by applying selected reaction monitoring, using 181.0→109.0 and 181.0→81.0 for quantification and confirmation purposes, respectively. The technique provides a sensitive and selective determination of myo-inositol in the analysed samples, with a run time of 4min. The limit of detection and quantification were 0.2 and 0.5mgkg−1, respectively. The method was applied to six fortified commercial milk powder samples containing myo-inositol amounts ranging from 290 to 2200mgkg−1.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2011.05.045