Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica
For the first time, the possibility of maintaining an intact human mitochondrial genome in a heterologous system in the mitochondria of yeast Yarrowia lipolytica is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in Y. lipolytica b...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2016-11, Vol.52 (6), p.663-672 |
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| container_title | Applied biochemistry and microbiology |
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| creator | Isakova, E. P. Deryabina, Yu. I. Belyakova, A. V. Biryukova, J. K. Teplova, V. V. Shevelev, A. B. |
| description | For the first time, the possibility of maintaining an intact human mitochondrial genome in a heterologous system in the mitochondria of yeast
Yarrowia lipolytica
is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in
Y. lipolytica
by an artificially induced ability of yeast mitochondria for homologous recombination is proposed. A method of introducing and using phenotypic selection markers for the presence or absence of defects in genes
tRNA-Lys
and
tRNA-Leu
of the mitochondrial genome is developed. The proposed system can be used to correct harmful mutations of the human mitochondrial genome associated with mitochondrial diseases and for preparative amplification of intact mitochondrial DNA with an adjusted sequence in yeast cells. The applicability of the new system for the correction of mutations in the genes of Lys- and Leu-specific tRNAs of the human mitochondrial genome associated with serious and widespread human mitochondrial diseases such as myoclonic epilepsy with lactic acidosis (MELAS) and myoclonic epilepsy with ragged-red fibers (MERRF) is shown. |
| doi_str_mv | 10.1134/S0003683816060065 |
| format | Article |
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Yarrowia lipolytica
is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in
Y. lipolytica
by an artificially induced ability of yeast mitochondria for homologous recombination is proposed. A method of introducing and using phenotypic selection markers for the presence or absence of defects in genes
tRNA-Lys
and
tRNA-Leu
of the mitochondrial genome is developed. The proposed system can be used to correct harmful mutations of the human mitochondrial genome associated with mitochondrial diseases and for preparative amplification of intact mitochondrial DNA with an adjusted sequence in yeast cells. The applicability of the new system for the correction of mutations in the genes of Lys- and Leu-specific tRNAs of the human mitochondrial genome associated with serious and widespread human mitochondrial diseases such as myoclonic epilepsy with lactic acidosis (MELAS) and myoclonic epilepsy with ragged-red fibers (MERRF) is shown.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683816060065</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Fibers ; Genetics ; Genomes ; Life Sciences ; Medical Microbiology ; Microbiology ; Mitochondria ; Mitochondrial DNA ; Mutation ; Ribonucleic acid ; RNA ; Yarrowia lipolytica ; Yeast ; Yeasts</subject><ispartof>Applied biochemistry and microbiology, 2016-11, Vol.52 (6), p.663-672</ispartof><rights>Pleiades Publishing, Inc. 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-725567fc1dbe11d3547e15d25df627c53603289c47febe674421b125057671f33</citedby><cites>FETCH-LOGICAL-c349t-725567fc1dbe11d3547e15d25df627c53603289c47febe674421b125057671f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683816060065$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683816060065$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids></links><search><creatorcontrib>Isakova, E. P.</creatorcontrib><creatorcontrib>Deryabina, Yu. I.</creatorcontrib><creatorcontrib>Belyakova, A. V.</creatorcontrib><creatorcontrib>Biryukova, J. K.</creatorcontrib><creatorcontrib>Teplova, V. V.</creatorcontrib><creatorcontrib>Shevelev, A. B.</creatorcontrib><title>Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>For the first time, the possibility of maintaining an intact human mitochondrial genome in a heterologous system in the mitochondria of yeast
Yarrowia lipolytica
is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in
Y. lipolytica
by an artificially induced ability of yeast mitochondria for homologous recombination is proposed. A method of introducing and using phenotypic selection markers for the presence or absence of defects in genes
tRNA-Lys
and
tRNA-Leu
of the mitochondrial genome is developed. The proposed system can be used to correct harmful mutations of the human mitochondrial genome associated with mitochondrial diseases and for preparative amplification of intact mitochondrial DNA with an adjusted sequence in yeast cells. The applicability of the new system for the correction of mutations in the genes of Lys- and Leu-specific tRNAs of the human mitochondrial genome associated with serious and widespread human mitochondrial diseases such as myoclonic epilepsy with lactic acidosis (MELAS) and myoclonic epilepsy with ragged-red fibers (MERRF) is shown.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Fibers</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>Mutation</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Yarrowia lipolytica</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1LxDAQhoMouFZ_gLeAFy_VfDRJe5RFV0HwoB70UrLtdDdLm6xJFum_N2U9iOJhvpjnfRkGoXNKrijlxfUzIYTLkpdUEkmIFAdoltoy54QVh2g2rfNpf4xOQtiksZJlNUPvC7AQTYPDGCIMuHMeD9rYmMLYFY5rwIOJrlk723qje7zeDdriFVg3ADYWj6BDxG_ae_dpNO7N1vVjctSn6KjTfYCz75qh17vbl_l9_vi0eJjfPOYNL6qYKyaEVF1D2yVQ2nJRKKCiZaLtJFON4JJwVlZNoTpYglRFweiSMkGEkop2nGfocu-79e5jByHWgwkN9L224HahpiVXnCmacoYufqEbt_M2XTdRVKpy4jJE91TjXQgeunrrzaD9WFNST9-u_3w7adheExJrV-B_OP8r-gIqfoAV</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Isakova, E. 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P.</au><au>Deryabina, Yu. I.</au><au>Belyakova, A. V.</au><au>Biryukova, J. K.</au><au>Teplova, V. V.</au><au>Shevelev, A. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2016-11-01</date><risdate>2016</risdate><volume>52</volume><issue>6</issue><spage>663</spage><epage>672</epage><pages>663-672</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>For the first time, the possibility of maintaining an intact human mitochondrial genome in a heterologous system in the mitochondria of yeast
Yarrowia lipolytica
is shown. A method for introducing directional changes into the structure of the mitochondrial human genome replicating in
Y. lipolytica
by an artificially induced ability of yeast mitochondria for homologous recombination is proposed. A method of introducing and using phenotypic selection markers for the presence or absence of defects in genes
tRNA-Lys
and
tRNA-Leu
of the mitochondrial genome is developed. The proposed system can be used to correct harmful mutations of the human mitochondrial genome associated with mitochondrial diseases and for preparative amplification of intact mitochondrial DNA with an adjusted sequence in yeast cells. The applicability of the new system for the correction of mutations in the genes of Lys- and Leu-specific tRNAs of the human mitochondrial genome associated with serious and widespread human mitochondrial diseases such as myoclonic epilepsy with lactic acidosis (MELAS) and myoclonic epilepsy with ragged-red fibers (MERRF) is shown.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683816060065</doi><tpages>10</tpages></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Fibers Genetics Genomes Life Sciences Medical Microbiology Microbiology Mitochondria Mitochondrial DNA Mutation Ribonucleic acid RNA Yarrowia lipolytica Yeast Yeasts |
| title | Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica |
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