Clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of Bipolaris sorokiniana without inducing resistance
BACKGROUND Several diseases threaten cereal production, and fungicides are therefore widely used. Biological control is an environmentally friendly alternative, and the fungus Clonostachys rosea is a versatile antagonist, effective against several plant diseases. We studied the ability of C. rosea t...
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| Veröffentlicht in: | Pest management science 2016-12, Vol.72 (12), p.2231-2239 |
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| creator | Jensen, Birgit Lübeck, Peter S Jørgensen, Hans JL |
| description | BACKGROUND
Several diseases threaten cereal production, and fungicides are therefore widely used. Biological control is an environmentally friendly alternative, and the fungus Clonostachys rosea is a versatile antagonist, effective against several plant diseases. We studied the ability of C. rosea to control barley leaf pathogens and the mechanisms behind the inhibition, emphasising induced resistance.
RESULTS
Under controlled conditions, spray application of C. rosea isolate IK726 to barley leaves reduced Bipolaris sorokiniana severity by up to 70% when applied 24 h before or simultaneously with the pathogen, whereas application 24 h after the pathogen had no effect. IK726 also reduced the sporulation capacity of B. sorokiniana. Microscopy of B. sorokiniana infection revealed that IK726 primarily inhibited conidial germination and appressorium formation, while further pathogen development and host defence reactions (papillae and fluorescent epidermal cells) were unaffected. Likewise, expression of defence‐related genes encoding PR proteins was unaltered. In addition to B. sorokiniana, IK726 also reduced infection by Drechslera teres and Rhynchosporium commune.
CONCLUSION
C. rosea acted as a protectant against three barley leaf pathogens. B. sorokiniana was directly inhibited by IK726, whereas induced resistance appeared not to be involved. Quantitative microscopy is a powerful tool for elucidating mechanisms involved in disease control. © 2016 Society of Chemical Industry |
| doi_str_mv | 10.1002/ps.4260 |
| format | Article |
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Several diseases threaten cereal production, and fungicides are therefore widely used. Biological control is an environmentally friendly alternative, and the fungus Clonostachys rosea is a versatile antagonist, effective against several plant diseases. We studied the ability of C. rosea to control barley leaf pathogens and the mechanisms behind the inhibition, emphasising induced resistance.
RESULTS
Under controlled conditions, spray application of C. rosea isolate IK726 to barley leaves reduced Bipolaris sorokiniana severity by up to 70% when applied 24 h before or simultaneously with the pathogen, whereas application 24 h after the pathogen had no effect. IK726 also reduced the sporulation capacity of B. sorokiniana. Microscopy of B. sorokiniana infection revealed that IK726 primarily inhibited conidial germination and appressorium formation, while further pathogen development and host defence reactions (papillae and fluorescent epidermal cells) were unaffected. Likewise, expression of defence‐related genes encoding PR proteins was unaltered. In addition to B. sorokiniana, IK726 also reduced infection by Drechslera teres and Rhynchosporium commune.
CONCLUSION
C. rosea acted as a protectant against three barley leaf pathogens. B. sorokiniana was directly inhibited by IK726, whereas induced resistance appeared not to be involved. Quantitative microscopy is a powerful tool for elucidating mechanisms involved in disease control. © 2016 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.4260</identifier><identifier>PMID: 26910381</identifier><identifier>CODEN: PMSCFC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>antibiosis ; Ascomycota - pathogenicity ; Ascomycota - physiology ; Barley ; Biological control ; Bipolaris sorokiniana ; Clonostachys rosea ; conidial germination ; Crop diseases ; Disease control ; Disease Resistance - physiology ; Drechslera teres ; Fungicides ; Gene Expression Regulation, Plant ; Hordeum - genetics ; Hordeum - microbiology ; Hordeum vulgare ; Host-Pathogen Interactions ; Hypocreales - physiology ; induced resistance ; Leaves ; Microscopy ; Pathogens ; Pesticides ; Plant diseases ; Plant Diseases - microbiology ; Plant Leaves - microbiology ; Plant resistance ; Spores, Fungal - physiology</subject><ispartof>Pest management science, 2016-12, Vol.72 (12), p.2231-2239</ispartof><rights>2016 Society of Chemical Industry</rights><rights>2016 Society of Chemical Industry.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3840-4709e83142db71dfc4f9a11c9ed41e279bfcf747e1184986eea62f93df2ca93c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.4260$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.4260$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26910381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jensen, Birgit</creatorcontrib><creatorcontrib>Lübeck, Peter S</creatorcontrib><creatorcontrib>Jørgensen, Hans JL</creatorcontrib><title>Clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of Bipolaris sorokiniana without inducing resistance</title><title>Pest management science</title><addtitle>Pest. Manag. Sci</addtitle><description>BACKGROUND
Several diseases threaten cereal production, and fungicides are therefore widely used. Biological control is an environmentally friendly alternative, and the fungus Clonostachys rosea is a versatile antagonist, effective against several plant diseases. We studied the ability of C. rosea to control barley leaf pathogens and the mechanisms behind the inhibition, emphasising induced resistance.
RESULTS
Under controlled conditions, spray application of C. rosea isolate IK726 to barley leaves reduced Bipolaris sorokiniana severity by up to 70% when applied 24 h before or simultaneously with the pathogen, whereas application 24 h after the pathogen had no effect. IK726 also reduced the sporulation capacity of B. sorokiniana. Microscopy of B. sorokiniana infection revealed that IK726 primarily inhibited conidial germination and appressorium formation, while further pathogen development and host defence reactions (papillae and fluorescent epidermal cells) were unaffected. Likewise, expression of defence‐related genes encoding PR proteins was unaltered. In addition to B. sorokiniana, IK726 also reduced infection by Drechslera teres and Rhynchosporium commune.
CONCLUSION
C. rosea acted as a protectant against three barley leaf pathogens. B. sorokiniana was directly inhibited by IK726, whereas induced resistance appeared not to be involved. Quantitative microscopy is a powerful tool for elucidating mechanisms involved in disease control. © 2016 Society of Chemical Industry</description><subject>antibiosis</subject><subject>Ascomycota - pathogenicity</subject><subject>Ascomycota - physiology</subject><subject>Barley</subject><subject>Biological control</subject><subject>Bipolaris sorokiniana</subject><subject>Clonostachys rosea</subject><subject>conidial germination</subject><subject>Crop diseases</subject><subject>Disease control</subject><subject>Disease Resistance - physiology</subject><subject>Drechslera teres</subject><subject>Fungicides</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hordeum - genetics</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Host-Pathogen Interactions</subject><subject>Hypocreales - physiology</subject><subject>induced resistance</subject><subject>Leaves</subject><subject>Microscopy</subject><subject>Pathogens</subject><subject>Pesticides</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - microbiology</subject><subject>Plant resistance</subject><subject>Spores, Fungal - physiology</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiNERUtBvAGyxAYJpfUtcbykIyhUbUFcRHeW45w0bjN2sB0N8yi8LY6mzIIVq-PL58_2f4riBcEnBGN6OsUTTmv8qDgiFa1LLmXzeD9ubg6LpzHeYYyllPRJcUhrSTBryFHxezV652PSZthGFHwEjQJ0s4GI4uQTakefzICsQ60OI2xRu82TwbY2WXeLpgATOEhBJ-sdug1-kwakXbecDvO4W_Y9OrOTH3WwWeuDv7fOaqfRxqbBzykb85WLL0C0-TXOwLPioNdjhOcP9bj4_v7dt9WH8vLT-cfV28vSsIbjkgssoWGE064VpOsN76UmxEjoOAEqZNubXnABhDQ5ihpA17SXrOup0ZIZdly83nmn4H_OEJNa22hgHLUDP0dFGiYYrThu_gOldV01gi_oq3_QOz8Hlz-yCClndXZm6uUDNbdr6NQU7FqHrfrbnwy82QEbm7Pf7xOslrarKaql7erz16VkutzROUL4tad1uFe1YKJSP67P1RW5qfjFtVBf2B9pqrEN</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Jensen, Birgit</creator><creator>Lübeck, Peter S</creator><creator>Jørgensen, Hans JL</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of Bipolaris sorokiniana without inducing resistance</title><author>Jensen, Birgit ; Lübeck, Peter S ; Jørgensen, Hans JL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3840-4709e83142db71dfc4f9a11c9ed41e279bfcf747e1184986eea62f93df2ca93c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>antibiosis</topic><topic>Ascomycota - pathogenicity</topic><topic>Ascomycota - physiology</topic><topic>Barley</topic><topic>Biological control</topic><topic>Bipolaris sorokiniana</topic><topic>Clonostachys rosea</topic><topic>conidial germination</topic><topic>Crop diseases</topic><topic>Disease control</topic><topic>Disease Resistance - physiology</topic><topic>Drechslera teres</topic><topic>Fungicides</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hordeum - genetics</topic><topic>Hordeum - microbiology</topic><topic>Hordeum vulgare</topic><topic>Host-Pathogen Interactions</topic><topic>Hypocreales - physiology</topic><topic>induced resistance</topic><topic>Leaves</topic><topic>Microscopy</topic><topic>Pathogens</topic><topic>Pesticides</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - microbiology</topic><topic>Plant resistance</topic><topic>Spores, Fungal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jensen, Birgit</creatorcontrib><creatorcontrib>Lübeck, Peter S</creatorcontrib><creatorcontrib>Jørgensen, Hans JL</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jensen, Birgit</au><au>Lübeck, Peter S</au><au>Jørgensen, Hans JL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of Bipolaris sorokiniana without inducing resistance</atitle><jtitle>Pest management science</jtitle><addtitle>Pest. Manag. Sci</addtitle><date>2016-12</date><risdate>2016</risdate><volume>72</volume><issue>12</issue><spage>2231</spage><epage>2239</epage><pages>2231-2239</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><coden>PMSCFC</coden><abstract>BACKGROUND
Several diseases threaten cereal production, and fungicides are therefore widely used. Biological control is an environmentally friendly alternative, and the fungus Clonostachys rosea is a versatile antagonist, effective against several plant diseases. We studied the ability of C. rosea to control barley leaf pathogens and the mechanisms behind the inhibition, emphasising induced resistance.
RESULTS
Under controlled conditions, spray application of C. rosea isolate IK726 to barley leaves reduced Bipolaris sorokiniana severity by up to 70% when applied 24 h before or simultaneously with the pathogen, whereas application 24 h after the pathogen had no effect. IK726 also reduced the sporulation capacity of B. sorokiniana. Microscopy of B. sorokiniana infection revealed that IK726 primarily inhibited conidial germination and appressorium formation, while further pathogen development and host defence reactions (papillae and fluorescent epidermal cells) were unaffected. Likewise, expression of defence‐related genes encoding PR proteins was unaltered. In addition to B. sorokiniana, IK726 also reduced infection by Drechslera teres and Rhynchosporium commune.
CONCLUSION
C. rosea acted as a protectant against three barley leaf pathogens. B. sorokiniana was directly inhibited by IK726, whereas induced resistance appeared not to be involved. Quantitative microscopy is a powerful tool for elucidating mechanisms involved in disease control. © 2016 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>26910381</pmid><doi>10.1002/ps.4260</doi><tpages>9</tpages></addata></record> |
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| subjects | antibiosis Ascomycota - pathogenicity Ascomycota - physiology Barley Biological control Bipolaris sorokiniana Clonostachys rosea conidial germination Crop diseases Disease control Disease Resistance - physiology Drechslera teres Fungicides Gene Expression Regulation, Plant Hordeum - genetics Hordeum - microbiology Hordeum vulgare Host-Pathogen Interactions Hypocreales - physiology induced resistance Leaves Microscopy Pathogens Pesticides Plant diseases Plant Diseases - microbiology Plant Leaves - microbiology Plant resistance Spores, Fungal - physiology |
| title | Clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of Bipolaris sorokiniana without inducing resistance |
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