Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration
Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit–fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to pl...
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| Veröffentlicht in: | Journal of dental research 2016-02, Vol.95 (2), p.206-214 |
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| creator | Yasui, T. Mabuchi, Y. Toriumi, H. Ebine, T. Niibe, K. Houlihan, D.D. Morikawa, S. Onizawa, K. Kawana, H. Akazawa, C. Suzuki, N. Nakagawa, T. Okano, H. Matsuzaki, Y. |
| description | Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit–fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue–specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp–derived LNGFRLow+THY-1High+ cells represent a highly enriched population of clonogenic cells—notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFRLow+THY-1High+ dental pulp–derived cells provide an excellent source of material for bone regenerative strategies. |
| doi_str_mv | 10.1177/0022034515610748 |
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However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue–specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp–derived LNGFRLow+THY-1High+ cells represent a highly enriched population of clonogenic cells—notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFRLow+THY-1High+ dental pulp–derived cells provide an excellent source of material for bone regenerative strategies.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/0022034515610748</identifier><identifier>PMID: 26494655</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Adipocytes ; Adult ; Animals ; Antigens, CD - analysis ; Bone Diseases - surgery ; Bone growth ; Bone Regeneration - physiology ; CD90 antigen ; Cell Culture Techniques ; Cell differentiation ; Cell Differentiation - physiology ; Cell Lineage ; Cell Proliferation ; Cell Separation - methods ; Cell surface ; Cells, Cultured ; Colony-Forming Units Assay ; Culture Media ; Dental pulp ; Dental Pulp - cytology ; Dentistry ; Fibroblasts ; Fibroblasts - physiology ; Flow cytometry ; Flow Cytometry - methods ; Growth factors ; Humans ; Male ; Mesenchymal Stromal Cells - physiology ; Mesenchyme ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Nerve Tissue Proteins - analysis ; Osteogenesis ; Osteogenesis - physiology ; Phenotypes ; Progenitor cells ; Receptors, Nerve Growth Factor - analysis ; Regeneration ; Stem Cell Transplantation - methods ; Stem cells ; Stem Cells - physiology ; Surface markers ; Thy-1 Antigens - analysis ; Transplantation ; Young Adult</subject><ispartof>Journal of dental research, 2016-02, Vol.95 (2), p.206-214</ispartof><rights>International & American Associations for Dental Research 2015</rights><rights>International & American Associations for Dental Research 2015.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-cb1d7f5797f848ba1547a6e111acc89180b9e48689679c4ec7267c721c75760c3</citedby><cites>FETCH-LOGICAL-c464t-cb1d7f5797f848ba1547a6e111acc89180b9e48689679c4ec7267c721c75760c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0022034515610748$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0022034515610748$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21799,27903,27904,43600,43601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26494655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yasui, T.</creatorcontrib><creatorcontrib>Mabuchi, Y.</creatorcontrib><creatorcontrib>Toriumi, H.</creatorcontrib><creatorcontrib>Ebine, T.</creatorcontrib><creatorcontrib>Niibe, K.</creatorcontrib><creatorcontrib>Houlihan, D.D.</creatorcontrib><creatorcontrib>Morikawa, S.</creatorcontrib><creatorcontrib>Onizawa, K.</creatorcontrib><creatorcontrib>Kawana, H.</creatorcontrib><creatorcontrib>Akazawa, C.</creatorcontrib><creatorcontrib>Suzuki, N.</creatorcontrib><creatorcontrib>Nakagawa, T.</creatorcontrib><creatorcontrib>Okano, H.</creatorcontrib><creatorcontrib>Matsuzaki, Y.</creatorcontrib><title>Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit–fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue–specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp–derived LNGFRLow+THY-1High+ cells represent a highly enriched population of clonogenic cells—notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFRLow+THY-1High+ dental pulp–derived cells provide an excellent source of material for bone regenerative strategies.</description><subject>Adipocytes</subject><subject>Adult</subject><subject>Animals</subject><subject>Antigens, CD - analysis</subject><subject>Bone Diseases - surgery</subject><subject>Bone growth</subject><subject>Bone Regeneration - physiology</subject><subject>CD90 antigen</subject><subject>Cell Culture Techniques</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage</subject><subject>Cell Proliferation</subject><subject>Cell Separation - methods</subject><subject>Cell surface</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Culture Media</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dentistry</subject><subject>Fibroblasts</subject><subject>Fibroblasts - physiology</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>Phenotypes</subject><subject>Progenitor cells</subject><subject>Receptors, Nerve Growth Factor - analysis</subject><subject>Regeneration</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Stem Cells - physiology</subject><subject>Surface markers</subject><subject>Thy-1 Antigens - analysis</subject><subject>Transplantation</subject><subject>Young Adult</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLw0AQxhdRbK3ePUnAi5fozmazj6PUaoVCi49z2G4nJSWPupsc_O_d0KpQEC8zh_nNN9_MEHIJ9BZAyjtKGaMJTyEVQCVXR2QIKecxTTUck2Ffjvv6gJx5v6EUNFPJKRkwwTUXaTokk0XnirzAVTTtKlNHD1i3powWXbmNXlusojGWpY8WrqmaFqO5b7FZY13Y6AVDRmfaoqnPyUluSo8X-zwi74-Tt_E0ns2fnsf3s9hywdvYLmEl81RqmSuuliZ4lUYgABhrlQZFlxq5EkoLqS1HK5mQIYCVqRTUJiNys9PduuajQ99mVeFtcGhqbDqfgUpkwrhi8n80CGoGWtGAXh-gm6ZzdVgkYwmlAlgie4ruKOsa7x3m2dYVlXGfGdCs_0Z2-I3QcrUX7pYVrn4avs8fgHgHeLPG36l_Cn4BCSCOHA</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Yasui, T.</creator><creator>Mabuchi, Y.</creator><creator>Toriumi, H.</creator><creator>Ebine, T.</creator><creator>Niibe, K.</creator><creator>Houlihan, D.D.</creator><creator>Morikawa, S.</creator><creator>Onizawa, K.</creator><creator>Kawana, H.</creator><creator>Akazawa, C.</creator><creator>Suzuki, N.</creator><creator>Nakagawa, T.</creator><creator>Okano, H.</creator><creator>Matsuzaki, Y.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20160201</creationdate><title>Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration</title><author>Yasui, T. ; Mabuchi, Y. ; Toriumi, H. ; Ebine, T. ; Niibe, K. ; Houlihan, D.D. ; Morikawa, S. ; Onizawa, K. ; Kawana, H. ; Akazawa, C. ; Suzuki, N. ; Nakagawa, T. ; Okano, H. ; Matsuzaki, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-cb1d7f5797f848ba1547a6e111acc89180b9e48689679c4ec7267c721c75760c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adipocytes</topic><topic>Adult</topic><topic>Animals</topic><topic>Antigens, CD - analysis</topic><topic>Bone Diseases - surgery</topic><topic>Bone growth</topic><topic>Bone Regeneration - physiology</topic><topic>CD90 antigen</topic><topic>Cell Culture Techniques</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Lineage</topic><topic>Cell Proliferation</topic><topic>Cell Separation - methods</topic><topic>Cell surface</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Culture Media</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dentistry</topic><topic>Fibroblasts</topic><topic>Fibroblasts - physiology</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Male</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mesenchyme</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Osteogenesis</topic><topic>Osteogenesis - physiology</topic><topic>Phenotypes</topic><topic>Progenitor cells</topic><topic>Receptors, Nerve Growth Factor - analysis</topic><topic>Regeneration</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem cells</topic><topic>Stem Cells - physiology</topic><topic>Surface markers</topic><topic>Thy-1 Antigens - analysis</topic><topic>Transplantation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yasui, T.</creatorcontrib><creatorcontrib>Mabuchi, Y.</creatorcontrib><creatorcontrib>Toriumi, H.</creatorcontrib><creatorcontrib>Ebine, T.</creatorcontrib><creatorcontrib>Niibe, K.</creatorcontrib><creatorcontrib>Houlihan, D.D.</creatorcontrib><creatorcontrib>Morikawa, S.</creatorcontrib><creatorcontrib>Onizawa, K.</creatorcontrib><creatorcontrib>Kawana, H.</creatorcontrib><creatorcontrib>Akazawa, C.</creatorcontrib><creatorcontrib>Suzuki, N.</creatorcontrib><creatorcontrib>Nakagawa, T.</creatorcontrib><creatorcontrib>Okano, H.</creatorcontrib><creatorcontrib>Matsuzaki, Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yasui, T.</au><au>Mabuchi, Y.</au><au>Toriumi, H.</au><au>Ebine, T.</au><au>Niibe, K.</au><au>Houlihan, D.D.</au><au>Morikawa, S.</au><au>Onizawa, K.</au><au>Kawana, H.</au><au>Akazawa, C.</au><au>Suzuki, N.</au><au>Nakagawa, T.</au><au>Okano, H.</au><au>Matsuzaki, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>95</volume><issue>2</issue><spage>206</spage><epage>214</epage><pages>206-214</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><abstract>Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit–fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue–specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp–derived LNGFRLow+THY-1High+ cells represent a highly enriched population of clonogenic cells—notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFRLow+THY-1High+ dental pulp–derived cells provide an excellent source of material for bone regenerative strategies.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>26494655</pmid><doi>10.1177/0022034515610748</doi><tpages>9</tpages></addata></record> |
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| subjects | Adipocytes Adult Animals Antigens, CD - analysis Bone Diseases - surgery Bone growth Bone Regeneration - physiology CD90 antigen Cell Culture Techniques Cell differentiation Cell Differentiation - physiology Cell Lineage Cell Proliferation Cell Separation - methods Cell surface Cells, Cultured Colony-Forming Units Assay Culture Media Dental pulp Dental Pulp - cytology Dentistry Fibroblasts Fibroblasts - physiology Flow cytometry Flow Cytometry - methods Growth factors Humans Male Mesenchymal Stromal Cells - physiology Mesenchyme Mice Mice, Inbred NOD Mice, SCID Nerve Tissue Proteins - analysis Osteogenesis Osteogenesis - physiology Phenotypes Progenitor cells Receptors, Nerve Growth Factor - analysis Regeneration Stem Cell Transplantation - methods Stem cells Stem Cells - physiology Surface markers Thy-1 Antigens - analysis Transplantation Young Adult |
| title | Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration |
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