A simplified ATP method for the rapid control of cell viability in a freeze-dried BCG vaccine

We propose a simple and cost-effective ATP method for controlling the specific activity of a freeze-dried BCG vaccine. A freeze-dried BCG vaccine is reconstituted with 1ml saline and incubated for 15min at room temperature and then for 1h at 37°C. The vaccine is then treated with apyrase to remove e...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2016-11, Vol.130, p.48-53
Hauptverfasser:	Ugarova, Natalia N., Lomakina, Galina Yu, Modestova, Yulia, Chernikov, Sergey V., Vinokurova, Natalia V., Оtrashevskaya, Elena V., Gorbachev, Vyacheslav Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analysis Adenosine Triphosphate - metabolism Apyrase - metabolism ATP assay Bacteriological Techniques - economics Bacteriological Techniques - methods BCG Vaccine - chemistry BCG viability Colony Count, Microbial Colony forming units Freeze Drying - methods Freeze-dried BCG vaccine Luminescent Measurements - methods Microbial Viability Mycobacterium bovis - growth & development Quality Control Temperature Time Factors Tuberculosis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!