Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs
A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Departme...
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| Veröffentlicht in: | Microbial ecology 2016-11, Vol.72 (4), p.840-850 |
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| creator | McArthur, J. V. Fletcher, D. E. Tuckfield, R. Cary Baker-Austin, C. |
| description | A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy’s Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin. |
| doi_str_mv | 10.1007/s00248-015-0678-4 |
| format | Article |
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To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. 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V.</au><au>Fletcher, D. E.</au><au>Tuckfield, R. Cary</au><au>Baker-Austin, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs</atitle><jtitle>Microbial ecology</jtitle><stitle>Microb Ecol</stitle><addtitle>Microb Ecol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>72</volume><issue>4</issue><spage>840</spage><epage>850</epage><pages>840-850</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><abstract>A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy’s Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin.</abstract><cop>New York</cop><pub>Springer Science + Business Media</pub><pmid>26530280</pmid><doi>10.1007/s00248-015-0678-4</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9892-9744</orcidid></addata></record> |
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| subjects | Aminoglycosides - pharmacology Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antimicrobial agents beta-Lactam Resistance - genetics beta-Lactams - pharmacology Biomedical and Life Sciences Ciprofloxacin - pharmacology Drug Combinations Drug Resistance, Multiple, Bacterial - physiology E coli Ecology ENVIRONMENTAL MICROBIOLOGY Escherichia coli Escherichia coli - drug effects Escherichia coli - isolation & purification Fluoroquinolones - pharmacology Geoecology/Natural Processes Geologic Sediments - microbiology Harbors Life Sciences Microbial Ecology Microbial Sensitivity Tests Microbiology Microorganisms Nature Conservation Rivers - microbiology Streams Water Microbiology Water Quality/Water Pollution |
| title | Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs |
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