Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition
•This study present the quantitative analysis of phenolic metabolites in A. keiskei.•Major component of in stems, chalcones also present abundantly in root barks.•The final output was to find that xanthine oxidase was inhibited by chalcones. Angelica keiskei is used as popular functional food stuff....
Gespeichert in:
| Veröffentlicht in: | Food chemistry 2014-06, Vol.153, p.20-27 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 27 |
|---|---|
| container_issue | |
| container_start_page | 20 |
| container_title | Food chemistry |
| container_volume | 153 |
| creator | Kim, Dae Wook Curtis-Long, Marcus J. Yuk, Heung Joo Wang, Yan Song, Yeong Hun Jeong, Seong Hun Park, Ki Hun |
| description | •This study present the quantitative analysis of phenolic metabolites in A. keiskei.•Major component of in stems, chalcones also present abundantly in root barks.•The final output was to find that xanthine oxidase was inhibited by chalcones.
Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant’s metabolites has not yet been disclosed. The principal phenolic compounds (1–16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1–16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4–44μg/kg and 1.5–148μg/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12–16) decreased as follows: root bark (10.51mg/g)>stems (8.52mg/g)>leaves (2.63mg/g)>root cores (1.44mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5μM. Chalcones (12–16) exhibited mixed-type inhibition characteristics. |
| doi_str_mv | 10.1016/j.foodchem.2013.12.026 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1837320070</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0308814613018815</els_id><sourcerecordid>1837320070</sourcerecordid><originalsourceid>FETCH-LOGICAL-c431t-12a980e759cea711982dc6183eb1a1250ed292b3df63fe62e67c55f46bb649fe3</originalsourceid><addsrcrecordid>eNqFkc9uEzEQhy0EomnhFSpfkLjs1n92vbs3qqjQSqkABc6W1zsmDrt2sJ2qufEISH1DngSHpHDswRofvvnNaD6EzikpKaHiYl0a7we9gqlkhPKSspIw8QzNaNvwoiENe45mhJO2aGklTtBpjGtCSGbbl-iEVVVHRVfP0K_PW-WSTSrZO8DKqXEXbcTe4M0KnB-txhMk1edfgohN8BMerDEQwCW8USH9hS_dN8iswt_Bxvxwv8PXnxbz3z8frpY3-HZ5cbvM6QNOK7AB3-eZK-sA-3s7qAjYupXtbbLevUIvjBojvD7WM_T1_dWX-XWx-PjhZn65KHTFaSooU11LoKk7DaqhtGvZoAVtOfRUUVYTGFjHej4YwQ0IBqLRdW0q0fei6gzwM_T2kLsJ_scWYpKTjRrGUTnw2yhzVMMZIQ15Gq26jnKeT5tRcUB18DEGMHIT7KTCTlIi9-LkWj6Kk3txkjKZxeXG8-OMbT_B8K_t0VQG3hwBFbUaTVBO2_ifazltO7bf4N2Bg3y8OwtBRm3BaRhsAJ3k4O1Tu_wB-z28GA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1499133018</pqid></control><display><type>article</type><title>Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Kim, Dae Wook ; Curtis-Long, Marcus J. ; Yuk, Heung Joo ; Wang, Yan ; Song, Yeong Hun ; Jeong, Seong Hun ; Park, Ki Hun</creator><creatorcontrib>Kim, Dae Wook ; Curtis-Long, Marcus J. ; Yuk, Heung Joo ; Wang, Yan ; Song, Yeong Hun ; Jeong, Seong Hun ; Park, Ki Hun</creatorcontrib><description>•This study present the quantitative analysis of phenolic metabolites in A. keiskei.•Major component of in stems, chalcones also present abundantly in root barks.•The final output was to find that xanthine oxidase was inhibited by chalcones.
Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant’s metabolites has not yet been disclosed. The principal phenolic compounds (1–16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1–16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4–44μg/kg and 1.5–148μg/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12–16) decreased as follows: root bark (10.51mg/g)>stems (8.52mg/g)>leaves (2.63mg/g)>root cores (1.44mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5μM. Chalcones (12–16) exhibited mixed-type inhibition characteristics.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2013.12.026</identifier><identifier>PMID: 24491695</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Angelica ; Angelica - chemistry ; Angelica - metabolism ; Angelica keiskei ; Biological and medical sciences ; Chalcone ; Chromatography, High Pressure Liquid - methods ; Coumarin ; Enzyme Inhibitors - analysis ; Enzyme Inhibitors - metabolism ; Food toxicology ; Humans ; Medical sciences ; MRM ; Phenols - analysis ; Phenols - metabolism ; Plant Extracts - analysis ; Plant Extracts - metabolism ; Tandem Mass Spectrometry - methods ; Toxicology ; Xanthine oxidase ; Xanthine Oxidase - analysis ; Xanthine Oxidase - antagonists & inhibitors ; Xanthine Oxidase - metabolism</subject><ispartof>Food chemistry, 2014-06, Vol.153, p.20-27</ispartof><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-12a980e759cea711982dc6183eb1a1250ed292b3df63fe62e67c55f46bb649fe3</citedby><cites>FETCH-LOGICAL-c431t-12a980e759cea711982dc6183eb1a1250ed292b3df63fe62e67c55f46bb649fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814613018815$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28318928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24491695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Dae Wook</creatorcontrib><creatorcontrib>Curtis-Long, Marcus J.</creatorcontrib><creatorcontrib>Yuk, Heung Joo</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Song, Yeong Hun</creatorcontrib><creatorcontrib>Jeong, Seong Hun</creatorcontrib><creatorcontrib>Park, Ki Hun</creatorcontrib><title>Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•This study present the quantitative analysis of phenolic metabolites in A. keiskei.•Major component of in stems, chalcones also present abundantly in root barks.•The final output was to find that xanthine oxidase was inhibited by chalcones.
Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant’s metabolites has not yet been disclosed. The principal phenolic compounds (1–16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1–16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4–44μg/kg and 1.5–148μg/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12–16) decreased as follows: root bark (10.51mg/g)>stems (8.52mg/g)>leaves (2.63mg/g)>root cores (1.44mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5μM. Chalcones (12–16) exhibited mixed-type inhibition characteristics.</description><subject>Angelica</subject><subject>Angelica - chemistry</subject><subject>Angelica - metabolism</subject><subject>Angelica keiskei</subject><subject>Biological and medical sciences</subject><subject>Chalcone</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Coumarin</subject><subject>Enzyme Inhibitors - analysis</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Food toxicology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>MRM</subject><subject>Phenols - analysis</subject><subject>Phenols - metabolism</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - metabolism</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Toxicology</subject><subject>Xanthine oxidase</subject><subject>Xanthine Oxidase - analysis</subject><subject>Xanthine Oxidase - antagonists & inhibitors</subject><subject>Xanthine Oxidase - metabolism</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9uEzEQhy0EomnhFSpfkLjs1n92vbs3qqjQSqkABc6W1zsmDrt2sJ2qufEISH1DngSHpHDswRofvvnNaD6EzikpKaHiYl0a7we9gqlkhPKSspIw8QzNaNvwoiENe45mhJO2aGklTtBpjGtCSGbbl-iEVVVHRVfP0K_PW-WSTSrZO8DKqXEXbcTe4M0KnB-txhMk1edfgohN8BMerDEQwCW8USH9hS_dN8iswt_Bxvxwv8PXnxbz3z8frpY3-HZ5cbvM6QNOK7AB3-eZK-sA-3s7qAjYupXtbbLevUIvjBojvD7WM_T1_dWX-XWx-PjhZn65KHTFaSooU11LoKk7DaqhtGvZoAVtOfRUUVYTGFjHej4YwQ0IBqLRdW0q0fei6gzwM_T2kLsJ_scWYpKTjRrGUTnw2yhzVMMZIQ15Gq26jnKeT5tRcUB18DEGMHIT7KTCTlIi9-LkWj6Kk3txkjKZxeXG8-OMbT_B8K_t0VQG3hwBFbUaTVBO2_ifazltO7bf4N2Bg3y8OwtBRm3BaRhsAJ3k4O1Tu_wB-z28GA</recordid><startdate>20140615</startdate><enddate>20140615</enddate><creator>Kim, Dae Wook</creator><creator>Curtis-Long, Marcus J.</creator><creator>Yuk, Heung Joo</creator><creator>Wang, Yan</creator><creator>Song, Yeong Hun</creator><creator>Jeong, Seong Hun</creator><creator>Park, Ki Hun</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20140615</creationdate><title>Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition</title><author>Kim, Dae Wook ; Curtis-Long, Marcus J. ; Yuk, Heung Joo ; Wang, Yan ; Song, Yeong Hun ; Jeong, Seong Hun ; Park, Ki Hun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-12a980e759cea711982dc6183eb1a1250ed292b3df63fe62e67c55f46bb649fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Angelica</topic><topic>Angelica - chemistry</topic><topic>Angelica - metabolism</topic><topic>Angelica keiskei</topic><topic>Biological and medical sciences</topic><topic>Chalcone</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Coumarin</topic><topic>Enzyme Inhibitors - analysis</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Food toxicology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>MRM</topic><topic>Phenols - analysis</topic><topic>Phenols - metabolism</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - metabolism</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Toxicology</topic><topic>Xanthine oxidase</topic><topic>Xanthine Oxidase - analysis</topic><topic>Xanthine Oxidase - antagonists & inhibitors</topic><topic>Xanthine Oxidase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Dae Wook</creatorcontrib><creatorcontrib>Curtis-Long, Marcus J.</creatorcontrib><creatorcontrib>Yuk, Heung Joo</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Song, Yeong Hun</creatorcontrib><creatorcontrib>Jeong, Seong Hun</creatorcontrib><creatorcontrib>Park, Ki Hun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Dae Wook</au><au>Curtis-Long, Marcus J.</au><au>Yuk, Heung Joo</au><au>Wang, Yan</au><au>Song, Yeong Hun</au><au>Jeong, Seong Hun</au><au>Park, Ki Hun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2014-06-15</date><risdate>2014</risdate><volume>153</volume><spage>20</spage><epage>27</epage><pages>20-27</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•This study present the quantitative analysis of phenolic metabolites in A. keiskei.•Major component of in stems, chalcones also present abundantly in root barks.•The final output was to find that xanthine oxidase was inhibited by chalcones.
Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant’s metabolites has not yet been disclosed. The principal phenolic compounds (1–16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1–16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4–44μg/kg and 1.5–148μg/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12–16) decreased as follows: root bark (10.51mg/g)>stems (8.52mg/g)>leaves (2.63mg/g)>root cores (1.44mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5μM. Chalcones (12–16) exhibited mixed-type inhibition characteristics.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24491695</pmid><doi>10.1016/j.foodchem.2013.12.026</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0308-8146 |
| ispartof | Food chemistry, 2014-06, Vol.153, p.20-27 |
| issn | 0308-8146 1873-7072 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1837320070 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Angelica Angelica - chemistry Angelica - metabolism Angelica keiskei Biological and medical sciences Chalcone Chromatography, High Pressure Liquid - methods Coumarin Enzyme Inhibitors - analysis Enzyme Inhibitors - metabolism Food toxicology Humans Medical sciences MRM Phenols - analysis Phenols - metabolism Plant Extracts - analysis Plant Extracts - metabolism Tandem Mass Spectrometry - methods Toxicology Xanthine oxidase Xanthine Oxidase - analysis Xanthine Oxidase - antagonists & inhibitors Xanthine Oxidase - metabolism |
| title | Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC–ESI MS/MS and their xanthine oxidase inhibition |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T09%3A53%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20analysis%20of%20phenolic%20metabolites%20from%20different%20parts%20of%20Angelica%20keiskei%20by%20HPLC%E2%80%93ESI%20MS/MS%20and%20their%20xanthine%20oxidase%20inhibition&rft.jtitle=Food%20chemistry&rft.au=Kim,%20Dae%20Wook&rft.date=2014-06-15&rft.volume=153&rft.spage=20&rft.epage=27&rft.pages=20-27&rft.issn=0308-8146&rft.eissn=1873-7072&rft.coden=FOCHDJ&rft_id=info:doi/10.1016/j.foodchem.2013.12.026&rft_dat=%3Cproquest_cross%3E1837320070%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1499133018&rft_id=info:pmid/24491695&rft_els_id=S0308814613018815&rfr_iscdi=true |