High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column
► The first report on the use of monolithic column in the HPLC determination of aflatoxins. ► Separation time was reduced to 3.7min compared to about 17min on a conventional column. ► Chilli and peanut samples were highly contaminated with aflatoxins B1 and G1. A simple and rapid high performance li...
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| Veröffentlicht in: | Food chemistry 2012-07, Vol.133 (2), p.489-496 |
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| creator | Khayoon, Wejdan Shakir Saad, Bahruddin Lee, Tien Ping Salleh, Baharuddin |
| description | ► The first report on the use of monolithic column in the HPLC determination of aflatoxins. ► Separation time was reduced to 3.7min compared to about 17min on a conventional column. ► Chilli and peanut samples were highly contaminated with aflatoxins B1 and G1.
A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7min using Chromolith® performance RP-18e (100–4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were |
| doi_str_mv | 10.1016/j.foodchem.2012.01.010 |
| format | Article |
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A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7min using Chromolith® performance RP-18e (100–4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography–tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2012.01.010</identifier><identifier>PMID: 25683424</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>aflatoxin B1 ; Aflatoxin B1 - analysis ; Aflatoxins ; Aflatoxins - analysis ; Arachis - chemistry ; Arachis hypogaea ; Biological and medical sciences ; Capsicum - chemistry ; Cereal and baking product industries ; Chromatography, High Pressure Liquid - methods ; derivatization ; fluorescence ; Food industries ; Food toxicology ; foods ; Foodstuffs ; Fundamental and applied biological sciences. Psychology ; High performance liquid chromatography ; Liquid chromatography–tandem mass spectrometry ; mass spectrometry ; monitoring ; Monolithic column ; Oryza - chemistry ; peanuts ; peppers ; quantitative analysis ; rice ; silica ; Silicon Dioxide ; solid phase extraction</subject><ispartof>Food chemistry, 2012-07, Vol.133 (2), p.489-496</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-c9c511180476cbd612679c38a31afdadc05b3f70145ff05861039d51111c6daf3</citedby><cites>FETCH-LOGICAL-c455t-c9c511180476cbd612679c38a31afdadc05b3f70145ff05861039d51111c6daf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2012.01.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25662457$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25683424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khayoon, Wejdan Shakir</creatorcontrib><creatorcontrib>Saad, Bahruddin</creatorcontrib><creatorcontrib>Lee, Tien Ping</creatorcontrib><creatorcontrib>Salleh, Baharuddin</creatorcontrib><title>High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>► The first report on the use of monolithic column in the HPLC determination of aflatoxins. ► Separation time was reduced to 3.7min compared to about 17min on a conventional column. ► Chilli and peanut samples were highly contaminated with aflatoxins B1 and G1.
A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7min using Chromolith® performance RP-18e (100–4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography–tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.</description><subject>aflatoxin B1</subject><subject>Aflatoxin B1 - analysis</subject><subject>Aflatoxins</subject><subject>Aflatoxins - analysis</subject><subject>Arachis - chemistry</subject><subject>Arachis hypogaea</subject><subject>Biological and medical sciences</subject><subject>Capsicum - chemistry</subject><subject>Cereal and baking product industries</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>derivatization</subject><subject>fluorescence</subject><subject>Food industries</subject><subject>Food toxicology</subject><subject>foods</subject><subject>Foodstuffs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High performance liquid chromatography</subject><subject>Liquid chromatography–tandem mass spectrometry</subject><subject>mass spectrometry</subject><subject>monitoring</subject><subject>Monolithic column</subject><subject>Oryza - chemistry</subject><subject>peanuts</subject><subject>peppers</subject><subject>quantitative analysis</subject><subject>rice</subject><subject>silica</subject><subject>Silicon Dioxide</subject><subject>solid phase extraction</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEQxS0EoiHwFYovSBy6wWPvejc3UEUpUiUO0LPl-E8ykddO7V0EZ744jpIibpVG8sG_995oHiGXwFbAQH7Yr3xK1uzcuOIM-IpBHfaMLGDoRdOznj8nCybY0AzQygvyqpQ9Y6yyw0tywTs5iJa3C_LnFrc7enDZpzzqaBwN-DCjpWaX06intM36sENDrZtcHjHqCVOkyVPtQ_3-hbFQjBXHEPCqOuk4T1RHSzNWt7lg3NKCAY2mG12cpWOKKeB0NDUpzGN8TV54HYp7c36X5P7m84_r2-bu25ev15_uGtN23dSYtekAYGBtL83GSuCyXxsxaAHaW20N6zbC9wzaznvWDRKYWNujBIy02osleX_yPeT0MLsyqRGLcSHo6NJcFAyiFyA62T6Nyq4XnEMrKipPqMmplOy8OmQcdf6tgKljV2qvHrtSx64UgzqsCi_PGfNmdPaf7LGcCrw7A7oYHXyu_WD5n5O8rXssydsT53VSepsrc_-9JrWspss1X1fi44lw9bw_0WVVDLratsXszKRswqe2_Qu6PMAs</recordid><startdate>20120715</startdate><enddate>20120715</enddate><creator>Khayoon, Wejdan Shakir</creator><creator>Saad, Bahruddin</creator><creator>Lee, Tien Ping</creator><creator>Salleh, Baharuddin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20120715</creationdate><title>High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column</title><author>Khayoon, Wejdan Shakir ; Saad, Bahruddin ; Lee, Tien Ping ; Salleh, Baharuddin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-c9c511180476cbd612679c38a31afdadc05b3f70145ff05861039d51111c6daf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>aflatoxin B1</topic><topic>Aflatoxin B1 - analysis</topic><topic>Aflatoxins</topic><topic>Aflatoxins - analysis</topic><topic>Arachis - chemistry</topic><topic>Arachis hypogaea</topic><topic>Biological and medical sciences</topic><topic>Capsicum - chemistry</topic><topic>Cereal and baking product industries</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>derivatization</topic><topic>fluorescence</topic><topic>Food industries</topic><topic>Food toxicology</topic><topic>foods</topic><topic>Foodstuffs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High performance liquid chromatography</topic><topic>Liquid chromatography–tandem mass spectrometry</topic><topic>mass spectrometry</topic><topic>monitoring</topic><topic>Monolithic column</topic><topic>Oryza - chemistry</topic><topic>peanuts</topic><topic>peppers</topic><topic>quantitative analysis</topic><topic>rice</topic><topic>silica</topic><topic>Silicon Dioxide</topic><topic>solid phase extraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khayoon, Wejdan Shakir</creatorcontrib><creatorcontrib>Saad, Bahruddin</creatorcontrib><creatorcontrib>Lee, Tien Ping</creatorcontrib><creatorcontrib>Salleh, Baharuddin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khayoon, Wejdan Shakir</au><au>Saad, Bahruddin</au><au>Lee, Tien Ping</au><au>Salleh, Baharuddin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2012-07-15</date><risdate>2012</risdate><volume>133</volume><issue>2</issue><spage>489</spage><epage>496</epage><pages>489-496</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>► The first report on the use of monolithic column in the HPLC determination of aflatoxins. ► Separation time was reduced to 3.7min compared to about 17min on a conventional column. ► Chilli and peanut samples were highly contaminated with aflatoxins B1 and G1.
A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7min using Chromolith® performance RP-18e (100–4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography–tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>25683424</pmid><doi>10.1016/j.foodchem.2012.01.010</doi><tpages>8</tpages></addata></record> |
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| subjects | aflatoxin B1 Aflatoxin B1 - analysis Aflatoxins Aflatoxins - analysis Arachis - chemistry Arachis hypogaea Biological and medical sciences Capsicum - chemistry Cereal and baking product industries Chromatography, High Pressure Liquid - methods derivatization fluorescence Food industries Food toxicology foods Foodstuffs Fundamental and applied biological sciences. Psychology High performance liquid chromatography Liquid chromatography–tandem mass spectrometry mass spectrometry monitoring Monolithic column Oryza - chemistry peanuts peppers quantitative analysis rice silica Silicon Dioxide solid phase extraction |
| title | High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column |
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