Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride
The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses. Animals were...
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| Veröffentlicht in: | Life sciences (1973) 2016-07, Vol.157, p.168-177 |
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| creator | Popović, Dejan Đukić, Davor Katić, Vukica Jović, Zorica Jović, Maja Lalić, Jelena Golubović, Ilija Stojanović, Svetlana Ulrih, Nataša Poklar Stanković, Marko Sokolović, Dušan |
| description | The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses.
Animals were divided into four groups: Group I (0.9% NaCl-10days), Group II (bilberry extract, 75mg/kg-10days), Group III (0,9% NaCl-9days, and on the tenth day CCl4–2ml/kg), Group IV (bilberry extract, 75mg/kg-10days and on the tenth day CCl4–2ml/kg).
Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum (AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p |
| doi_str_mv | 10.1016/j.lfs.2016.06.007 |
| format | Article |
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Animals were divided into four groups: Group I (0.9% NaCl-10days), Group II (bilberry extract, 75mg/kg-10days), Group III (0,9% NaCl-9days, and on the tenth day CCl4–2ml/kg), Group IV (bilberry extract, 75mg/kg-10days and on the tenth day CCl4–2ml/kg).
Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum (AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p<0.01). Bilberry extract resulted in a significant increase in the activity of the antioxidant markers-catalase (p<0.05), superoxide dismutase, glutathione S-transferase and glutathione peroxidase (p<0.01), and the concentration of reduced glutathione (p<0.05) in Group IV in relation to Group III. The application of bilberry extract resulted in an increase in the number of apoptotic hepatocytes and the activity of caspase-3 in the liver tissue (p<0.01). The reduction of coagulation necrotic areas was proved (p<0.001) as well as the number of macrovesicular hepatocytes (p<0.01), along with an increased mitotic activity (p<0.01) in Group IV compared to Group III.
Anthocyanins from bilberry extract have strong antioxidant properties and therefore can be considered as powerful hepatoprotectives in natural products.]]></description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2016.06.007</identifier><identifier>PMID: 27312419</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Anthocyanins ; Anthocyanins - pharmacology ; Antioxidant ; Antioxidants - pharmacology ; Apoptosis - drug effects ; Bilberry ; Carbon tetrachloride ; Carbon Tetrachloride - toxicity ; Lipid Peroxidation - drug effects ; Liver - drug effects ; Liver - pathology ; Plant Extracts - pharmacology ; Proapoptotic activity ; Rats ; Rats, Wistar ; Vaccinium myrtillus - chemistry</subject><ispartof>Life sciences (1973), 2016-07, Vol.157, p.168-177</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-13aef947451297057bfe0e05b405426f81b7470c206c3003d646529a662c6ae43</citedby><cites>FETCH-LOGICAL-c452t-13aef947451297057bfe0e05b405426f81b7470c206c3003d646529a662c6ae43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320516303630$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27312419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Popović, Dejan</creatorcontrib><creatorcontrib>Đukić, Davor</creatorcontrib><creatorcontrib>Katić, Vukica</creatorcontrib><creatorcontrib>Jović, Zorica</creatorcontrib><creatorcontrib>Jović, Maja</creatorcontrib><creatorcontrib>Lalić, Jelena</creatorcontrib><creatorcontrib>Golubović, Ilija</creatorcontrib><creatorcontrib>Stojanović, Svetlana</creatorcontrib><creatorcontrib>Ulrih, Nataša Poklar</creatorcontrib><creatorcontrib>Stanković, Marko</creatorcontrib><creatorcontrib>Sokolović, Dušan</creatorcontrib><title>Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description><![CDATA[The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses.
Animals were divided into four groups: Group I (0.9% NaCl-10days), Group II (bilberry extract, 75mg/kg-10days), Group III (0,9% NaCl-9days, and on the tenth day CCl4–2ml/kg), Group IV (bilberry extract, 75mg/kg-10days and on the tenth day CCl4–2ml/kg).
Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum (AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p<0.01). Bilberry extract resulted in a significant increase in the activity of the antioxidant markers-catalase (p<0.05), superoxide dismutase, glutathione S-transferase and glutathione peroxidase (p<0.01), and the concentration of reduced glutathione (p<0.05) in Group IV in relation to Group III. The application of bilberry extract resulted in an increase in the number of apoptotic hepatocytes and the activity of caspase-3 in the liver tissue (p<0.01). The reduction of coagulation necrotic areas was proved (p<0.001) as well as the number of macrovesicular hepatocytes (p<0.01), along with an increased mitotic activity (p<0.01) in Group IV compared to Group III.
Anthocyanins from bilberry extract have strong antioxidant properties and therefore can be considered as powerful hepatoprotectives in natural products.]]></description><subject>Animals</subject><subject>Anthocyanins</subject><subject>Anthocyanins - pharmacology</subject><subject>Antioxidant</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Bilberry</subject><subject>Carbon tetrachloride</subject><subject>Carbon Tetrachloride - toxicity</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>Plant Extracts - pharmacology</subject><subject>Proapoptotic activity</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vaccinium myrtillus - chemistry</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1qGzEUhUVpaJy0D9BN0bKbca80-pmhqxDSJhDoplkLjeYKy4xHU0ku9iP0rSvjNNBNKVyQQN85F51DyHsGawZMfdquJ5_XvF7XUAf0K7Jine4bUC17TVYAXDQtB3lJrnLeAoCUun1DLrluGResX5FfN3MJ8RBGOxdq55EuKdolLiWW4Ch6j65kGn19K5vojnYOc6Y-xR0dwjRgSkeKh5KsKzTMNNlK42GJGUdaIt3gYqtVXfCXmbNpiDMteBJuppjCiG_JhbdTxnfP5zV5-nL3_fa-efz29eH25rFxQvLSsNai74UWkvFeg9SDR0CQgwApuPIdG7TQ4Dgo1wK0oxJK8t4qxZ2yKNpr8vHsW3_6Y4-5mF3IDqfJzhj32bCuPaXTye4_UFCgO8ZVRdkZdSnmnNCbJYWdTUfDwJzKMltTyzKnsgzUAV01H57t98MOxxfFn3Yq8PkMYM3jZ8Bksgs4OxxDqlGaMYZ_2P8GSDqmew</recordid><startdate>20160715</startdate><enddate>20160715</enddate><creator>Popović, Dejan</creator><creator>Đukić, Davor</creator><creator>Katić, Vukica</creator><creator>Jović, Zorica</creator><creator>Jović, Maja</creator><creator>Lalić, Jelena</creator><creator>Golubović, Ilija</creator><creator>Stojanović, Svetlana</creator><creator>Ulrih, Nataša Poklar</creator><creator>Stanković, Marko</creator><creator>Sokolović, Dušan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20160715</creationdate><title>Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride</title><author>Popović, Dejan ; Đukić, Davor ; Katić, Vukica ; Jović, Zorica ; Jović, Maja ; Lalić, Jelena ; Golubović, Ilija ; Stojanović, Svetlana ; Ulrih, Nataša Poklar ; Stanković, Marko ; Sokolović, Dušan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-13aef947451297057bfe0e05b405426f81b7470c206c3003d646529a662c6ae43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anthocyanins</topic><topic>Anthocyanins - pharmacology</topic><topic>Antioxidant</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Bilberry</topic><topic>Carbon tetrachloride</topic><topic>Carbon Tetrachloride - toxicity</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Plant Extracts - pharmacology</topic><topic>Proapoptotic activity</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vaccinium myrtillus - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Popović, Dejan</creatorcontrib><creatorcontrib>Đukić, Davor</creatorcontrib><creatorcontrib>Katić, Vukica</creatorcontrib><creatorcontrib>Jović, Zorica</creatorcontrib><creatorcontrib>Jović, Maja</creatorcontrib><creatorcontrib>Lalić, Jelena</creatorcontrib><creatorcontrib>Golubović, Ilija</creatorcontrib><creatorcontrib>Stojanović, Svetlana</creatorcontrib><creatorcontrib>Ulrih, Nataša Poklar</creatorcontrib><creatorcontrib>Stanković, Marko</creatorcontrib><creatorcontrib>Sokolović, Dušan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Popović, Dejan</au><au>Đukić, Davor</au><au>Katić, Vukica</au><au>Jović, Zorica</au><au>Jović, Maja</au><au>Lalić, Jelena</au><au>Golubović, Ilija</au><au>Stojanović, Svetlana</au><au>Ulrih, Nataša Poklar</au><au>Stanković, Marko</au><au>Sokolović, Dušan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2016-07-15</date><risdate>2016</risdate><volume>157</volume><spage>168</spage><epage>177</epage><pages>168-177</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract><![CDATA[The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses.
Animals were divided into four groups: Group I (0.9% NaCl-10days), Group II (bilberry extract, 75mg/kg-10days), Group III (0,9% NaCl-9days, and on the tenth day CCl4–2ml/kg), Group IV (bilberry extract, 75mg/kg-10days and on the tenth day CCl4–2ml/kg).
Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum (AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p<0.01). Bilberry extract resulted in a significant increase in the activity of the antioxidant markers-catalase (p<0.05), superoxide dismutase, glutathione S-transferase and glutathione peroxidase (p<0.01), and the concentration of reduced glutathione (p<0.05) in Group IV in relation to Group III. The application of bilberry extract resulted in an increase in the number of apoptotic hepatocytes and the activity of caspase-3 in the liver tissue (p<0.01). The reduction of coagulation necrotic areas was proved (p<0.001) as well as the number of macrovesicular hepatocytes (p<0.01), along with an increased mitotic activity (p<0.01) in Group IV compared to Group III.
Anthocyanins from bilberry extract have strong antioxidant properties and therefore can be considered as powerful hepatoprotectives in natural products.]]></abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>27312419</pmid><doi>10.1016/j.lfs.2016.06.007</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Anthocyanins Anthocyanins - pharmacology Antioxidant Antioxidants - pharmacology Apoptosis - drug effects Bilberry Carbon tetrachloride Carbon Tetrachloride - toxicity Lipid Peroxidation - drug effects Liver - drug effects Liver - pathology Plant Extracts - pharmacology Proapoptotic activity Rats Rats, Wistar Vaccinium myrtillus - chemistry |
| title | Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride |
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