Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12
We collected 69 ticks from nine, free-ranging Amur tigers (Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were ob...
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| Veröffentlicht in: | Journal of wildlife diseases 2016-07, Vol.52 (3), p.674-676 |
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| container_title | Journal of wildlife diseases |
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| creator | Thomas, Lindsay H. Seryodkin, Ivan V. Goodrich, John M. Miquelle, Dale G. Birtles, Richard J. Lewis, John C. M. |
| description | We collected 69 ticks from nine, free-ranging Amur tigers (Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East. |
| doi_str_mv | 10.7589/2015-10-277 |
| format | Article |
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M.</creator><creatorcontrib>Thomas, Lindsay H. ; Seryodkin, Ivan V. ; Goodrich, John M. ; Miquelle, Dale G. ; Birtles, Richard J. ; Lewis, John C. M.</creatorcontrib><description>We collected 69 ticks from nine, free-ranging Amur tigers (Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.</description><identifier>ISSN: 0090-3558</identifier><identifier>EISSN: 1943-3700</identifier><identifier>DOI: 10.7589/2015-10-277</identifier><identifier>PMID: 27243154</identifier><language>eng</language><publisher>United States: Wildife Disease Association</publisher><subject>Amur tiger ; Animals ; Apicomplexa - isolation & purification ; Felis ; Hepatozoon ; Hepatozoon felis ; Ixodidae ; Panthera tigris altaica ; Russia - epidemiology ; Russian Far East ; SHORT COMMUNICATIONS ; Tick Infestations - epidemiology ; Tick Infestations - parasitology ; Tick Infestations - veterinary ; tick-borne disease ; Ticks - parasitology ; Tigers</subject><ispartof>Journal of wildlife diseases, 2016-07, Vol.52 (3), p.674-676</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b356t-db4db4aed8d546404251c92d4f95123911b1bca063c4f2ab3a918146b025167b3</citedby><cites>FETCH-LOGICAL-b356t-db4db4aed8d546404251c92d4f95123911b1bca063c4f2ab3a918146b025167b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27243154$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, Lindsay H.</creatorcontrib><creatorcontrib>Seryodkin, Ivan V.</creatorcontrib><creatorcontrib>Goodrich, John M.</creatorcontrib><creatorcontrib>Miquelle, Dale G.</creatorcontrib><creatorcontrib>Birtles, Richard J.</creatorcontrib><creatorcontrib>Lewis, John C. M.</creatorcontrib><title>Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12</title><title>Journal of wildlife diseases</title><addtitle>J Wildl Dis</addtitle><description>We collected 69 ticks from nine, free-ranging Amur tigers (Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.</description><subject>Amur tiger</subject><subject>Animals</subject><subject>Apicomplexa - isolation & purification</subject><subject>Felis</subject><subject>Hepatozoon</subject><subject>Hepatozoon felis</subject><subject>Ixodidae</subject><subject>Panthera tigris altaica</subject><subject>Russia - epidemiology</subject><subject>Russian Far East</subject><subject>SHORT COMMUNICATIONS</subject><subject>Tick Infestations - epidemiology</subject><subject>Tick Infestations - parasitology</subject><subject>Tick Infestations - veterinary</subject><subject>tick-borne disease</subject><subject>Ticks - parasitology</subject><subject>Tigers</subject><issn>0090-3558</issn><issn>1943-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r3DAQhkVoaTZpT7kXHRMSNxp9WPYxbLNJIdAQ0rMZ2fJWrW1tJfmQnnLJL-g_7C-Jlk17LIWB4YWHlxkeQo6AfdCqqs85A1UAK7jWe2QBtRSF0Iy9IgvGalYIpap9chDjN8a4yuEN2eeaSwFKLsjTR5tsm5yfqO_ptd1g8j99Tr0dXKRuoveu_R7p0g9D5mxH--BHugrWFnc4rd20phfjHDK2tiHS41uc0lcbkCa3DrkBh4SuxZMzejfH6HCiKwz0EmM6ozxf9PvxF_C35HWPQ7TvXvYh-bK6vF9eFzefrz4tL24KI1SZis7IPGi7qlOylExyBW3NO9nXCrioAQyYFlkpWtlzNAJrqECWJj8OpTbikBzvejfB_5htTM3oYmuHASfr59hAJTSvRaXhP1CmleCiUhk93aFt8DEG2zeb4EYMDw2wZquo2Srahqwo0-9fimcz2u4v-8dJBk52gHFZhP1n2TOFA5ap</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Thomas, Lindsay H.</creator><creator>Seryodkin, Ivan V.</creator><creator>Goodrich, John M.</creator><creator>Miquelle, Dale G.</creator><creator>Birtles, Richard J.</creator><creator>Lewis, John C. M.</creator><general>Wildife Disease Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SN</scope><scope>C1K</scope></search><sort><creationdate>201607</creationdate><title>Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12</title><author>Thomas, Lindsay H. ; Seryodkin, Ivan V. ; Goodrich, John M. ; Miquelle, Dale G. ; Birtles, Richard J. ; Lewis, John C. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b356t-db4db4aed8d546404251c92d4f95123911b1bca063c4f2ab3a918146b025167b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amur tiger</topic><topic>Animals</topic><topic>Apicomplexa - isolation & purification</topic><topic>Felis</topic><topic>Hepatozoon</topic><topic>Hepatozoon felis</topic><topic>Ixodidae</topic><topic>Panthera tigris altaica</topic><topic>Russia - epidemiology</topic><topic>Russian Far East</topic><topic>SHORT COMMUNICATIONS</topic><topic>Tick Infestations - epidemiology</topic><topic>Tick Infestations - parasitology</topic><topic>Tick Infestations - veterinary</topic><topic>tick-borne disease</topic><topic>Ticks - parasitology</topic><topic>Tigers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, Lindsay H.</creatorcontrib><creatorcontrib>Seryodkin, Ivan V.</creatorcontrib><creatorcontrib>Goodrich, John M.</creatorcontrib><creatorcontrib>Miquelle, Dale G.</creatorcontrib><creatorcontrib>Birtles, Richard J.</creatorcontrib><creatorcontrib>Lewis, John C. 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M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12</atitle><jtitle>Journal of wildlife diseases</jtitle><addtitle>J Wildl Dis</addtitle><date>2016-07</date><risdate>2016</risdate><volume>52</volume><issue>3</issue><spage>674</spage><epage>676</epage><pages>674-676</pages><issn>0090-3558</issn><eissn>1943-3700</eissn><abstract>We collected 69 ticks from nine, free-ranging Amur tigers (Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.</abstract><cop>United States</cop><pub>Wildife Disease Association</pub><pmid>27243154</pmid><doi>10.7589/2015-10-277</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-3558 |
| ispartof | Journal of wildlife diseases, 2016-07, Vol.52 (3), p.674-676 |
| issn | 0090-3558 1943-3700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1837293871 |
| source | MEDLINE; Allen Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amur tiger Animals Apicomplexa - isolation & purification Felis Hepatozoon Hepatozoon felis Ixodidae Panthera tigris altaica Russia - epidemiology Russian Far East SHORT COMMUNICATIONS Tick Infestations - epidemiology Tick Infestations - parasitology Tick Infestations - veterinary tick-borne disease Ticks - parasitology Tigers |
| title | Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12 |
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