Reorganization of plasma membrane lipid domains during conidial germination
Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane c...
Gespeichert in:
Veröffentlicht in: | Biochimica et biophysica acta. Molecular and cell biology of lipids 2017-02, Vol.1862 (2), p.156-166 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 166 |
---|---|
container_issue | 2 |
container_start_page | 156 |
container_title | Biochimica et biophysica acta. Molecular and cell biology of lipids |
container_volume | 1862 |
creator | Santos, Filipa C. Fernandes, Andreia S. Antunes, Catarina A.C. Moreira, Filipe P. Videira, Arnaldo Marinho, H. Susana de Almeida, Rodrigo F.M. |
description | Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane composition of N. crassa occur during spore germination. However, the biophysical impact of those changes is unknown. Thus, a biophysical study of N. crassa plasma membrane, particularly SL-enriched domains, and their dynamics along conidial germination is prompted.
Two N. crassa strains, wild-type (WT) and slime, which is devoid of cell wall, were studied. Conidial growth of N. crassa WT from a dormancy state to an exponential phase was accompanied by membrane reorganization, namely an increase of membrane fluidity, occurring faster in a supplemented medium than in Vogel's minimal medium. Gel-like domains, likely enriched in SLs, were found in both N. crassa strains, but were particularly compact, rigid and abundant in the case of slime cells, even more than in budding yeast Saccharomyces cerevisiae. In N. crassa, our results suggest that the melting of SL-enriched domains occurs near growth temperature (30°C) for WT, but at higher temperatures for slime. Regarding biophysical properties strongly affected by ergosterol, the plasma membrane of slime conidia lays in between those of N. crassa WT and S. cerevisiae cells. The differences in biophysical properties found in this work, and the relationships established between membrane lipid composition and dynamics, give new insights about the plasma membrane organization and structure of N. crassa strains during conidial growth.
[Display omitted]
•Conidia germination includes major reorganization and fluidization of the membrane.•N. crassa conidia lack ergosterol, but contain sphingolipid (SL)-enriched domains.•Those domains are gel-like, melting near growth temperature for N. crassa wild type.•Cell wall-less mutant (slime) unable to form hyphae has more and tighter SL domains.•Slime membrane biophysical properties resemble those of unicellular S. cerevisiae. |
doi_str_mv | 10.1016/j.bbalip.2016.10.011 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1836732705</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1388198116302980</els_id><sourcerecordid>1836732705</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-251dd0f53abe8da208d98793ec96cd0b0e87e9e27b8ebc9c01ef8aed39e551c03</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMorq7-A5EevbTmw7bpRZDFL1wQRM8hTaZLliapyVbQX2_Wrh49ZTK877wzD0JnBBcEk-pyXbSt7M1Q0PRLrQITsoeOCK-bnFaE76eacZ6ThpMZOo5xjTEpGSsP0YzWnJSU0iP09AI-rKQzX3JjvMt8lw29jFZmFmwbpIMsZRidaW-lcTHTYzBulSnvjDayz1YQrHE_5hN00Mk-wununaO3u9vXxUO-fL5_XNwsc8UquslpSbTGXclkC1xLirlu0tIMVFMpjVsMvIYGaN1yaFWjMIGOS9CsgbIkCrM5upjmDsG_jxA3wpqooO_Ttn6MgnBW1YzWuEzSq0mqgo8xQCeGYKwMn4JgscUo1mLCKLYYt92EMdnOdwlja0H_mX65JcH1JIB054eBIKIy4BRoE0BthPbm_4Rvt_WGrA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1836732705</pqid></control><display><type>article</type><title>Reorganization of plasma membrane lipid domains during conidial germination</title><source>MEDLINE</source><source>ScienceDirect Freedom Collection (Elsevier)</source><creator>Santos, Filipa C. ; Fernandes, Andreia S. ; Antunes, Catarina A.C. ; Moreira, Filipe P. ; Videira, Arnaldo ; Marinho, H. Susana ; de Almeida, Rodrigo F.M.</creator><creatorcontrib>Santos, Filipa C. ; Fernandes, Andreia S. ; Antunes, Catarina A.C. ; Moreira, Filipe P. ; Videira, Arnaldo ; Marinho, H. Susana ; de Almeida, Rodrigo F.M.</creatorcontrib><description>Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane composition of N. crassa occur during spore germination. However, the biophysical impact of those changes is unknown. Thus, a biophysical study of N. crassa plasma membrane, particularly SL-enriched domains, and their dynamics along conidial germination is prompted.
Two N. crassa strains, wild-type (WT) and slime, which is devoid of cell wall, were studied. Conidial growth of N. crassa WT from a dormancy state to an exponential phase was accompanied by membrane reorganization, namely an increase of membrane fluidity, occurring faster in a supplemented medium than in Vogel's minimal medium. Gel-like domains, likely enriched in SLs, were found in both N. crassa strains, but were particularly compact, rigid and abundant in the case of slime cells, even more than in budding yeast Saccharomyces cerevisiae. In N. crassa, our results suggest that the melting of SL-enriched domains occurs near growth temperature (30°C) for WT, but at higher temperatures for slime. Regarding biophysical properties strongly affected by ergosterol, the plasma membrane of slime conidia lays in between those of N. crassa WT and S. cerevisiae cells. The differences in biophysical properties found in this work, and the relationships established between membrane lipid composition and dynamics, give new insights about the plasma membrane organization and structure of N. crassa strains during conidial growth.
[Display omitted]
•Conidia germination includes major reorganization and fluidization of the membrane.•N. crassa conidia lack ergosterol, but contain sphingolipid (SL)-enriched domains.•Those domains are gel-like, melting near growth temperature for N. crassa wild type.•Cell wall-less mutant (slime) unable to form hyphae has more and tighter SL domains.•Slime membrane biophysical properties resemble those of unicellular S. cerevisiae.</description><identifier>ISSN: 1388-1981</identifier><identifier>ISSN: 1879-2618</identifier><identifier>EISSN: 1879-2618</identifier><identifier>DOI: 10.1016/j.bbalip.2016.10.011</identifier><identifier>PMID: 27815222</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cell Membrane - metabolism ; Cell Membrane - physiology ; Cell Wall - metabolism ; Cell Wall - physiology ; Ergosterol ; Fluorescence spectroscopy ; Fungal Proteins - metabolism ; Fungal sphingolipids ; Lipid domains/rafts ; Membrane Fluidity - physiology ; Membrane Lipids - metabolism ; Membranes - metabolism ; Membranes - physiology ; Neurospora crassa ; Neurospora crassa - growth & development ; Neurospora crassa - metabolism ; Neurospora crassa - physiology ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae - physiology ; Sphingolipids - metabolism ; Spores - growth & development ; Spores - metabolism ; Spores - physiology ; Spores, Fungal - growth & development ; Spores, Fungal - metabolism ; Spores, Fungal - physiology</subject><ispartof>Biochimica et biophysica acta. Molecular and cell biology of lipids, 2017-02, Vol.1862 (2), p.156-166</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-251dd0f53abe8da208d98793ec96cd0b0e87e9e27b8ebc9c01ef8aed39e551c03</citedby><cites>FETCH-LOGICAL-c362t-251dd0f53abe8da208d98793ec96cd0b0e87e9e27b8ebc9c01ef8aed39e551c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbalip.2016.10.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27815222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos, Filipa C.</creatorcontrib><creatorcontrib>Fernandes, Andreia S.</creatorcontrib><creatorcontrib>Antunes, Catarina A.C.</creatorcontrib><creatorcontrib>Moreira, Filipe P.</creatorcontrib><creatorcontrib>Videira, Arnaldo</creatorcontrib><creatorcontrib>Marinho, H. Susana</creatorcontrib><creatorcontrib>de Almeida, Rodrigo F.M.</creatorcontrib><title>Reorganization of plasma membrane lipid domains during conidial germination</title><title>Biochimica et biophysica acta. Molecular and cell biology of lipids</title><addtitle>Biochim Biophys Acta Mol Cell Biol Lipids</addtitle><description>Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane composition of N. crassa occur during spore germination. However, the biophysical impact of those changes is unknown. Thus, a biophysical study of N. crassa plasma membrane, particularly SL-enriched domains, and their dynamics along conidial germination is prompted.
Two N. crassa strains, wild-type (WT) and slime, which is devoid of cell wall, were studied. Conidial growth of N. crassa WT from a dormancy state to an exponential phase was accompanied by membrane reorganization, namely an increase of membrane fluidity, occurring faster in a supplemented medium than in Vogel's minimal medium. Gel-like domains, likely enriched in SLs, were found in both N. crassa strains, but were particularly compact, rigid and abundant in the case of slime cells, even more than in budding yeast Saccharomyces cerevisiae. In N. crassa, our results suggest that the melting of SL-enriched domains occurs near growth temperature (30°C) for WT, but at higher temperatures for slime. Regarding biophysical properties strongly affected by ergosterol, the plasma membrane of slime conidia lays in between those of N. crassa WT and S. cerevisiae cells. The differences in biophysical properties found in this work, and the relationships established between membrane lipid composition and dynamics, give new insights about the plasma membrane organization and structure of N. crassa strains during conidial growth.
[Display omitted]
•Conidia germination includes major reorganization and fluidization of the membrane.•N. crassa conidia lack ergosterol, but contain sphingolipid (SL)-enriched domains.•Those domains are gel-like, melting near growth temperature for N. crassa wild type.•Cell wall-less mutant (slime) unable to form hyphae has more and tighter SL domains.•Slime membrane biophysical properties resemble those of unicellular S. cerevisiae.</description><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - physiology</subject><subject>Cell Wall - metabolism</subject><subject>Cell Wall - physiology</subject><subject>Ergosterol</subject><subject>Fluorescence spectroscopy</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungal sphingolipids</subject><subject>Lipid domains/rafts</subject><subject>Membrane Fluidity - physiology</subject><subject>Membrane Lipids - metabolism</subject><subject>Membranes - metabolism</subject><subject>Membranes - physiology</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - growth & development</subject><subject>Neurospora crassa - metabolism</subject><subject>Neurospora crassa - physiology</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Sphingolipids - metabolism</subject><subject>Spores - growth & development</subject><subject>Spores - metabolism</subject><subject>Spores - physiology</subject><subject>Spores, Fungal - growth & development</subject><subject>Spores, Fungal - metabolism</subject><subject>Spores, Fungal - physiology</subject><issn>1388-1981</issn><issn>1879-2618</issn><issn>1879-2618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMorq7-A5EevbTmw7bpRZDFL1wQRM8hTaZLliapyVbQX2_Wrh49ZTK877wzD0JnBBcEk-pyXbSt7M1Q0PRLrQITsoeOCK-bnFaE76eacZ6ThpMZOo5xjTEpGSsP0YzWnJSU0iP09AI-rKQzX3JjvMt8lw29jFZmFmwbpIMsZRidaW-lcTHTYzBulSnvjDayz1YQrHE_5hN00Mk-wununaO3u9vXxUO-fL5_XNwsc8UquslpSbTGXclkC1xLirlu0tIMVFMpjVsMvIYGaN1yaFWjMIGOS9CsgbIkCrM5upjmDsG_jxA3wpqooO_Ttn6MgnBW1YzWuEzSq0mqgo8xQCeGYKwMn4JgscUo1mLCKLYYt92EMdnOdwlja0H_mX65JcH1JIB054eBIKIy4BRoE0BthPbm_4Rvt_WGrA</recordid><startdate>201702</startdate><enddate>201702</enddate><creator>Santos, Filipa C.</creator><creator>Fernandes, Andreia S.</creator><creator>Antunes, Catarina A.C.</creator><creator>Moreira, Filipe P.</creator><creator>Videira, Arnaldo</creator><creator>Marinho, H. Susana</creator><creator>de Almeida, Rodrigo F.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201702</creationdate><title>Reorganization of plasma membrane lipid domains during conidial germination</title><author>Santos, Filipa C. ; Fernandes, Andreia S. ; Antunes, Catarina A.C. ; Moreira, Filipe P. ; Videira, Arnaldo ; Marinho, H. Susana ; de Almeida, Rodrigo F.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-251dd0f53abe8da208d98793ec96cd0b0e87e9e27b8ebc9c01ef8aed39e551c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - physiology</topic><topic>Cell Wall - metabolism</topic><topic>Cell Wall - physiology</topic><topic>Ergosterol</topic><topic>Fluorescence spectroscopy</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungal sphingolipids</topic><topic>Lipid domains/rafts</topic><topic>Membrane Fluidity - physiology</topic><topic>Membrane Lipids - metabolism</topic><topic>Membranes - metabolism</topic><topic>Membranes - physiology</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - growth & development</topic><topic>Neurospora crassa - metabolism</topic><topic>Neurospora crassa - physiology</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Sphingolipids - metabolism</topic><topic>Spores - growth & development</topic><topic>Spores - metabolism</topic><topic>Spores - physiology</topic><topic>Spores, Fungal - growth & development</topic><topic>Spores, Fungal - metabolism</topic><topic>Spores, Fungal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos, Filipa C.</creatorcontrib><creatorcontrib>Fernandes, Andreia S.</creatorcontrib><creatorcontrib>Antunes, Catarina A.C.</creatorcontrib><creatorcontrib>Moreira, Filipe P.</creatorcontrib><creatorcontrib>Videira, Arnaldo</creatorcontrib><creatorcontrib>Marinho, H. Susana</creatorcontrib><creatorcontrib>de Almeida, Rodrigo F.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimica et biophysica acta. Molecular and cell biology of lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Filipa C.</au><au>Fernandes, Andreia S.</au><au>Antunes, Catarina A.C.</au><au>Moreira, Filipe P.</au><au>Videira, Arnaldo</au><au>Marinho, H. Susana</au><au>de Almeida, Rodrigo F.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reorganization of plasma membrane lipid domains during conidial germination</atitle><jtitle>Biochimica et biophysica acta. Molecular and cell biology of lipids</jtitle><addtitle>Biochim Biophys Acta Mol Cell Biol Lipids</addtitle><date>2017-02</date><risdate>2017</risdate><volume>1862</volume><issue>2</issue><spage>156</spage><epage>166</epage><pages>156-166</pages><issn>1388-1981</issn><issn>1879-2618</issn><eissn>1879-2618</eissn><abstract>Neurospora crassa, a filamentous fungus, in the unicellular conidial stage has ideal features to study sphingolipid (SL)-enriched domains, which are implicated in fundamental cellular processes ranging from antifungal resistance to apoptosis. Several changes in lipid metabolism and in the membrane composition of N. crassa occur during spore germination. However, the biophysical impact of those changes is unknown. Thus, a biophysical study of N. crassa plasma membrane, particularly SL-enriched domains, and their dynamics along conidial germination is prompted.
Two N. crassa strains, wild-type (WT) and slime, which is devoid of cell wall, were studied. Conidial growth of N. crassa WT from a dormancy state to an exponential phase was accompanied by membrane reorganization, namely an increase of membrane fluidity, occurring faster in a supplemented medium than in Vogel's minimal medium. Gel-like domains, likely enriched in SLs, were found in both N. crassa strains, but were particularly compact, rigid and abundant in the case of slime cells, even more than in budding yeast Saccharomyces cerevisiae. In N. crassa, our results suggest that the melting of SL-enriched domains occurs near growth temperature (30°C) for WT, but at higher temperatures for slime. Regarding biophysical properties strongly affected by ergosterol, the plasma membrane of slime conidia lays in between those of N. crassa WT and S. cerevisiae cells. The differences in biophysical properties found in this work, and the relationships established between membrane lipid composition and dynamics, give new insights about the plasma membrane organization and structure of N. crassa strains during conidial growth.
[Display omitted]
•Conidia germination includes major reorganization and fluidization of the membrane.•N. crassa conidia lack ergosterol, but contain sphingolipid (SL)-enriched domains.•Those domains are gel-like, melting near growth temperature for N. crassa wild type.•Cell wall-less mutant (slime) unable to form hyphae has more and tighter SL domains.•Slime membrane biophysical properties resemble those of unicellular S. cerevisiae.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27815222</pmid><doi>10.1016/j.bbalip.2016.10.011</doi><tpages>11</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1388-1981 |
ispartof | Biochimica et biophysica acta. Molecular and cell biology of lipids, 2017-02, Vol.1862 (2), p.156-166 |
issn | 1388-1981 1879-2618 1879-2618 |
language | eng |
recordid | cdi_proquest_miscellaneous_1836732705 |
source | MEDLINE; ScienceDirect Freedom Collection (Elsevier) |
subjects | Cell Membrane - metabolism Cell Membrane - physiology Cell Wall - metabolism Cell Wall - physiology Ergosterol Fluorescence spectroscopy Fungal Proteins - metabolism Fungal sphingolipids Lipid domains/rafts Membrane Fluidity - physiology Membrane Lipids - metabolism Membranes - metabolism Membranes - physiology Neurospora crassa Neurospora crassa - growth & development Neurospora crassa - metabolism Neurospora crassa - physiology Saccharomyces cerevisiae Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Sphingolipids - metabolism Spores - growth & development Spores - metabolism Spores - physiology Spores, Fungal - growth & development Spores, Fungal - metabolism Spores, Fungal - physiology |
title | Reorganization of plasma membrane lipid domains during conidial germination |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A51%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reorganization%20of%20plasma%20membrane%20lipid%20domains%20during%20conidial%20germination&rft.jtitle=Biochimica%20et%20biophysica%20acta.%20Molecular%20and%20cell%20biology%20of%20lipids&rft.au=Santos,%20Filipa%20C.&rft.date=2017-02&rft.volume=1862&rft.issue=2&rft.spage=156&rft.epage=166&rft.pages=156-166&rft.issn=1388-1981&rft.eissn=1879-2618&rft_id=info:doi/10.1016/j.bbalip.2016.10.011&rft_dat=%3Cproquest_cross%3E1836732705%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1836732705&rft_id=info:pmid/27815222&rft_els_id=S1388198116302980&rfr_iscdi=true |