Set of Novel Automated Quantitative Microproteomics Protocols for Small Sample Amounts and Its Application to Kidney Tissue Substructures

Here we assessed the ability of an automated sample preparation device equipped with disposable microcolumns to prepare mass-limited samples for high-sensitivity quantitative proteomics, using both label-free and isobaric labeling approaches. First, we compared peptide label-free quantification repr...

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Veröffentlicht in:	Journal of proteome research 2016-12, Vol.15 (12), p.4722-4730
Hauptverfasser:	de Graaf, Erik Leonardus, Pellegrini, Davide, McDonnell, Liam A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Kidney - chemistry Kidney - ultrastructure Kidney Cortex - chemistry Kidney Medulla - chemistry Laser Capture Microdissection Mice Proteome - analysis Proteomics - methods Reproducibility of Results Sample Size Staining and Labeling
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