Implementation of a Low-Cost Automated LED Photometer for Enzymatic Reaction Detection to Teach Basic Bioelectronics Technologies in Vocational High Schools

This study proposes a home-assembled, low-cost blue light-emitting diode (LED) photometer that uses simple and low-cost hardware and software, costing about US 150. This 425-nm wavelength photometer is controlled by an 89C51 microcontroller chip. Glucose concentration detection experiments involving...

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Veröffentlicht in:IEEE transactions on education 2016-08, Vol.59 (3), p.194-201
Hauptverfasser: Chen, Huai-Yi, Nieh, Hwa-Ming, Yang, Ming-Feng, Chou, Yu-Kung, Chung, Jui-Hsu, Liou, Je-Wen
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container_issue 3
container_start_page 194
container_title IEEE transactions on education
container_volume 59
creator Chen, Huai-Yi
Nieh, Hwa-Ming
Yang, Ming-Feng
Chou, Yu-Kung
Chung, Jui-Hsu
Liou, Je-Wen
description This study proposes a home-assembled, low-cost blue light-emitting diode (LED) photometer that uses simple and low-cost hardware and software, costing about US 150. This 425-nm wavelength photometer is controlled by an 89C51 microcontroller chip. Glucose concentration detection experiments involving enzyme coupling reactions were carried out to verify the performance of the proposed system. Measurement errors for the system were between 3% and 4%, which can be further improved to less than 2%. The proposed LED photometer can be used by students in electronics classes to conduct biotechnological tests as part of their electrical and electronics vocational high school education. This can help them to explore the basic applications of biosensors and enhance their professional abilities in executing fundamental bioelectronics processes. Pretest and post-test assessments of students' knowledge of bioelectronics were conducted to determine the efficacy of the proposed system in enhancing students' knowledge. The LED photometer can be modified to test various samples in biochemistry and analytical chemistry, and it can also be applied to various fields such as electronics, chemical engineering, and biochemistry.
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This 425-nm wavelength photometer is controlled by an 89C51 microcontroller chip. Glucose concentration detection experiments involving enzyme coupling reactions were carried out to verify the performance of the proposed system. Measurement errors for the system were between 3% and 4%, which can be further improved to less than 2%. The proposed LED photometer can be used by students in electronics classes to conduct biotechnological tests as part of their electrical and electronics vocational high school education. This can help them to explore the basic applications of biosensors and enhance their professional abilities in executing fundamental bioelectronics processes. Pretest and post-test assessments of students' knowledge of bioelectronics were conducted to determine the efficacy of the proposed system in enhancing students' knowledge. 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This 425-nm wavelength photometer is controlled by an 89C51 microcontroller chip. Glucose concentration detection experiments involving enzyme coupling reactions were carried out to verify the performance of the proposed system. Measurement errors for the system were between 3% and 4%, which can be further improved to less than 2%. The proposed LED photometer can be used by students in electronics classes to conduct biotechnological tests as part of their electrical and electronics vocational high school education. This can help them to explore the basic applications of biosensors and enhance their professional abilities in executing fundamental bioelectronics processes. Pretest and post-test assessments of students' knowledge of bioelectronics were conducted to determine the efficacy of the proposed system in enhancing students' knowledge. 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subjects Absorption
Automatic testing
Biochemistry
Bioelectricity
biomedical measurement
Biosensors
Biotechnology
Chemistry
Education
electronic engineering education
Electronics
Energy
Foreign Countries
Hardware
High School Students
Knowledge Level
Light emitting diodes
light-emitting diodes (LEDs)
Measurement Equipment
microcontroller
Optical switches
Organic Chemistry
Photometers
Pretests Posttests
Science Experiments
Spectroscopy
Statistical Analysis
Student Evaluation
student experiment
Student Satisfaction
Student Surveys
Students
Vocational Education
Vocational High Schools
title Implementation of a Low-Cost Automated LED Photometer for Enzymatic Reaction Detection to Teach Basic Bioelectronics Technologies in Vocational High Schools
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