Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin

In the present study, a novel molecular biosensor system model was designed by using a couple of the fluorescent unnatural mutant streptavidin and the carbazole‐labeled biotin. BODIPY‐FL‐aminophenylalanine (BFLAF), a fluorescent unnatural amino acid was position‐specifically incorporated into Trp120...

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Veröffentlicht in:	Journal of molecular recognition 2016-10, Vol.29 (10), p.485-491
Hauptverfasser:	Zhu, Xianwei, Shinohara, Hiroaki, Miyatake, Ryuta, Hohsaka, Takahiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques Biosensors Biotin Biotin - chemistry Biotin - metabolism carbazole-labeled biotin Carbazoles Carbazoles - chemistry Carbazoles - metabolism Couples Fluorescence fluorescence quenching fluorescence recovery Fluorescent Dyes - chemistry fluorescent unnatural mutant streptavidin four-base codon method Mathematical models Models, Molecular molecular biosensor Mutation Protein Conformation Quenching Quenching (cooling) Spectrometry, Fluorescence Streptavidin - chemistry Streptavidin - genetics Streptavidin - metabolism Tryptophan - chemistry Tryptophan - genetics Tryptophan - metabolism
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