Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells
Abstract Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic different...
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| Veröffentlicht in: | Biomaterials 2016-10, Vol.103, p.86-100 |
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| creator | Kanninen, Liisa K Harjumäki, Riina Peltoniemi, Pasi Bogacheva, Mariia S Salmi, Tuuli Porola, Pauliina Niklander, Johanna Smutný, Tomáš Urtti, Arto Yliperttula, Marjo L Lou, Yan-Ru |
| description | Abstract Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells. |
| doi_str_mv | 10.1016/j.biomaterials.2016.06.054 |
| format | Article |
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Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2016.06.054</identifier><identifier>PMID: 27372423</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Batch Cell Culture Techniques - methods ; Biomaterials ; Biomimetic Materials - chemistry ; Cell Differentiation - physiology ; Cell Line ; Dentistry ; Drugs ; Extracellular matrix ; Extracellular Matrix - metabolism ; Extracellular Matrix Proteins - metabolism ; Fibronectin ; Hepatic differentiation ; Hepatocytes - cytology ; Hepatocytes - physiology ; Human embryonic stem cell ; Human induced pluripotent stem cell ; Humans ; In vitro testing ; Laminin - metabolism ; Laminin-511 ; Laminin-521 ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Proteins ; Specifications ; Stem cells ; Surgical implants ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2016-10, Vol.103, p.86-100</ispartof><rights>The Author(s)</rights><rights>2016 The Author(s)</rights><rights>Copyright © 2016 The Author(s). Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c619t-abd2c0f287eeb4d0790abc4c1f206eb024014cd6ac7e4cb12c1474bd948ed48d3</citedby><cites>FETCH-LOGICAL-c619t-abd2c0f287eeb4d0790abc4c1f206eb024014cd6ac7e4cb12c1474bd948ed48d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2016.06.054$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27372423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanninen, Liisa K</creatorcontrib><creatorcontrib>Harjumäki, Riina</creatorcontrib><creatorcontrib>Peltoniemi, Pasi</creatorcontrib><creatorcontrib>Bogacheva, Mariia S</creatorcontrib><creatorcontrib>Salmi, Tuuli</creatorcontrib><creatorcontrib>Porola, Pauliina</creatorcontrib><creatorcontrib>Niklander, Johanna</creatorcontrib><creatorcontrib>Smutný, Tomáš</creatorcontrib><creatorcontrib>Urtti, Arto</creatorcontrib><creatorcontrib>Yliperttula, Marjo L</creatorcontrib><creatorcontrib>Lou, Yan-Ru</creatorcontrib><title>Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. 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Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells.</description><subject>Advanced Basic Science</subject><subject>Batch Cell Culture Techniques - methods</subject><subject>Biomaterials</subject><subject>Biomimetic Materials - chemistry</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Dentistry</subject><subject>Drugs</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Fibronectin</subject><subject>Hepatic differentiation</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - physiology</subject><subject>Human embryonic stem cell</subject><subject>Human induced pluripotent stem cell</subject><subject>Humans</subject><subject>In vitro testing</subject><subject>Laminin - metabolism</subject><subject>Laminin-511</subject><subject>Laminin-521</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Proteins</subject><subject>Specifications</subject><subject>Stem cells</subject><subject>Surgical implants</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUstqHTEMNaWhuU37C8V01c3cWh57Hl0UStJH4EIXbdfGDw3x7Yw9tWcC-ft4uEkpXQUEQuJIR9IRIW-B7YFB8_64Nz5OesHk9Zj3vOT2rJgUz8gOurarZM_kc7JjIHjVN8DPycucj6zETPAX5Jy3dcsFr3ckHPTkgw-VBKA6ODo-xhwqozM6WpiSt5jpEBPFYfDWY1joDc568ZbmGa0vyRLEQONAb9ZJBzqPa_JzXDZoXnCiFscxvyJnQ5kZXz_4C_Lry-efl9-qw_ev15efDpVtoF8qbRy3bOBdi2iEY23PtLHCwsBZg4ZxUVaxrtG2RWENcAuiFcb1okMnOldfkHenvnOKf1bMi5p83ibQAeOaFXS1bGQtZfcEKJSbSibqAv1wgtoUc044qDn5Sac7BUxt0qij-lcatUmjWDEpSvGbB57VTOj-lj5qUQBXJwCWw9x6TCpvl7bofEK7KBf903g-_tfGjkVRq8ffeIf5GNcUthpQmSumfmxPsv0INDWroWx5DysGvDo</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Kanninen, Liisa K</creator><creator>Harjumäki, Riina</creator><creator>Peltoniemi, Pasi</creator><creator>Bogacheva, Mariia S</creator><creator>Salmi, Tuuli</creator><creator>Porola, Pauliina</creator><creator>Niklander, Johanna</creator><creator>Smutný, Tomáš</creator><creator>Urtti, Arto</creator><creator>Yliperttula, Marjo L</creator><creator>Lou, Yan-Ru</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20161001</creationdate><title>Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells</title><author>Kanninen, Liisa K ; Harjumäki, Riina ; Peltoniemi, Pasi ; Bogacheva, Mariia S ; Salmi, Tuuli ; Porola, Pauliina ; Niklander, Johanna ; Smutný, Tomáš ; Urtti, Arto ; Yliperttula, Marjo L ; Lou, Yan-Ru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c619t-abd2c0f287eeb4d0790abc4c1f206eb024014cd6ac7e4cb12c1474bd948ed48d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Advanced Basic Science</topic><topic>Batch Cell Culture Techniques - methods</topic><topic>Biomaterials</topic><topic>Biomimetic Materials - chemistry</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Dentistry</topic><topic>Drugs</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Fibronectin</topic><topic>Hepatic differentiation</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - physiology</topic><topic>Human embryonic stem cell</topic><topic>Human induced pluripotent stem cell</topic><topic>Humans</topic><topic>In vitro testing</topic><topic>Laminin - metabolism</topic><topic>Laminin-511</topic><topic>Laminin-521</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Proteins</topic><topic>Specifications</topic><topic>Stem cells</topic><topic>Surgical implants</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanninen, Liisa K</creatorcontrib><creatorcontrib>Harjumäki, Riina</creatorcontrib><creatorcontrib>Peltoniemi, Pasi</creatorcontrib><creatorcontrib>Bogacheva, Mariia S</creatorcontrib><creatorcontrib>Salmi, Tuuli</creatorcontrib><creatorcontrib>Porola, Pauliina</creatorcontrib><creatorcontrib>Niklander, Johanna</creatorcontrib><creatorcontrib>Smutný, Tomáš</creatorcontrib><creatorcontrib>Urtti, Arto</creatorcontrib><creatorcontrib>Yliperttula, Marjo L</creatorcontrib><creatorcontrib>Lou, Yan-Ru</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanninen, Liisa K</au><au>Harjumäki, Riina</au><au>Peltoniemi, Pasi</au><au>Bogacheva, Mariia S</au><au>Salmi, Tuuli</au><au>Porola, Pauliina</au><au>Niklander, Johanna</au><au>Smutný, Tomáš</au><au>Urtti, Arto</au><au>Yliperttula, Marjo L</au><au>Lou, Yan-Ru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>103</volume><spage>86</spage><epage>100</epage><pages>86-100</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>27372423</pmid><doi>10.1016/j.biomaterials.2016.06.054</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Advanced Basic Science Batch Cell Culture Techniques - methods Biomaterials Biomimetic Materials - chemistry Cell Differentiation - physiology Cell Line Dentistry Drugs Extracellular matrix Extracellular Matrix - metabolism Extracellular Matrix Proteins - metabolism Fibronectin Hepatic differentiation Hepatocytes - cytology Hepatocytes - physiology Human embryonic stem cell Human induced pluripotent stem cell Humans In vitro testing Laminin - metabolism Laminin-511 Laminin-521 Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Proteins Specifications Stem cells Surgical implants Tissue Engineering - methods |
| title | Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells |
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