Continuous genetic recording with self-targeting CRISPR-Cas in human cells

The ability to record molecular events in vivo would enable monitoring of signaling dynamics within cellular niches and critical factors that orchestrate cellular behavior. We present a self-contained analog memory device for longitudinal recording of molecular stimuli into DNA mutations in human ce...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2016-09, Vol.353 (6304), p.1115-1115
Hauptverfasser:	Perli, Samuel D., Cui, Cheryl H., Lu, Timothy K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - metabolism Base Sequence - genetics Cell Tracking - methods Cellular biology Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Protein 9 CRISPR-Cas Systems Cytology Data storage Deoxyribonucleic acid Devices DNA DNA - genetics Endonucleases - metabolism Genetics HEK293 Cells Humans In vivo methods and tests Memory Multiplexing Mutagenesis Recorders Recording RESEARCH ARTICLE SUMMARY RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism Signal monitoring Single-Cell Analysis - methods Streptococcus pyogenes Technology
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