In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells
Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selecte...
Gespeichert in:
| Veröffentlicht in: | Faraday discussions 2014-01, Vol.175, p.189-21 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 21 |
|---|---|
| container_issue | |
| container_start_page | 189 |
| container_title | Faraday discussions |
| container_volume | 175 |
| creator | Lesniak, A Kilinc, D Rashdan, Suad A von Kriegsheim, A Ashall, B Zerulla, D Kolch, W Lee, G. U |
| description | Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selected to be 150-200 nm so that they did not enter the cells. The 80 nm-long Au tips of the nanorods were functionalized with heregulin (HRG), and the micron-long Fe portion was coated with a poly(ethylene glycol) monolayer to minimize non-specific interactions. Nanorods functionalized with HRG were found to preferentially bind to MCF7 cells that express high levels of the receptor tyrosine-protein kinase ErbB2/3. Magnetic tweezers measurements were used to characterize the kinetic properties of the bond between the HRG on the rods and ErbB2/3 on the surface of the cells. The strong magnetization of Fe-Au nanorods makes them excellent candidates for
in-vitro
and
in-vivo
imaging, and magnetic therapeutic applications targeting cancer cells in circulation. |
| doi_str_mv | 10.1039/c4fd00115j |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835592334</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1835592334</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-34ee11a57b35386ef7fee0575f5022fda5ca8d6523e5236c0b9ff8432177d31c3</originalsourceid><addsrcrecordid>eNqF0c9PFDEUB_DGQATRi3dNvRmSan_PzHFZWMGw8aLnSbd9ZUtm27XtYPCvd5ZFvMmhP_LeJ69Nvgi9ZfQTo6L7bKV3lDKmbl-gYya0JEp27cHurjqitaRH6FUpt5RSPXVfoiOuuNaNFsfo7iriu1BzwqWO7h4nj-sacIgVsrE1pLgrrSHDzTiESPwYH6pmCL_B4Y25iVCDJWk77WbA0cSUkyv4V6hrvJwvGmyiw8vzGVmeES4YtjAM5TU69GYo8ObxPEE_Fhff55fk-tuXq_nsmlhJZSVCAjBmVLMSSrQafOMBqGqUV5Rz74yypnVacQHT0pauOu9bKThrGieYFSfo437uNqefI5Tab0LZ_cBESGPpWSuU6rgQ8nk6PSM140pP9HRPbU6lZPD9NoeNyfc9o_0ukn4uF-cPkXyd8PvHueNqA-6J_s1gAh_2IBf71P2Xab91fjLv_mfEH1zKmrA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1652461256</pqid></control><display><type>article</type><title>In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals</source><source>Alma/SFX Local Collection</source><creator>Lesniak, A ; Kilinc, D ; Rashdan, Suad A ; von Kriegsheim, A ; Ashall, B ; Zerulla, D ; Kolch, W ; Lee, G. U</creator><creatorcontrib>Lesniak, A ; Kilinc, D ; Rashdan, Suad A ; von Kriegsheim, A ; Ashall, B ; Zerulla, D ; Kolch, W ; Lee, G. U</creatorcontrib><description>Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selected to be 150-200 nm so that they did not enter the cells. The 80 nm-long Au tips of the nanorods were functionalized with heregulin (HRG), and the micron-long Fe portion was coated with a poly(ethylene glycol) monolayer to minimize non-specific interactions. Nanorods functionalized with HRG were found to preferentially bind to MCF7 cells that express high levels of the receptor tyrosine-protein kinase ErbB2/3. Magnetic tweezers measurements were used to characterize the kinetic properties of the bond between the HRG on the rods and ErbB2/3 on the surface of the cells. The strong magnetization of Fe-Au nanorods makes them excellent candidates for
in-vitro
and
in-vivo
imaging, and magnetic therapeutic applications targeting cancer cells in circulation.</description><identifier>ISSN: 1359-6640</identifier><identifier>EISSN: 1364-5498</identifier><identifier>DOI: 10.1039/c4fd00115j</identifier><identifier>PMID: 25266763</identifier><language>eng</language><publisher>England</publisher><subject>Cancer ; Cell Line, Tumor ; Gold - chemistry ; Humans ; Imaging ; Iron - chemistry ; Kinases ; Lab-On-A-Chip Devices ; Magnetic Fields ; Magnetic properties ; Magnetization ; MCF-7 Cells ; Nanorods ; Nanotubes - chemistry ; Neuregulin-1 - chemistry ; Optical Tweezers ; Rods ; Synthesis (chemistry)</subject><ispartof>Faraday discussions, 2014-01, Vol.175, p.189-21</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-34ee11a57b35386ef7fee0575f5022fda5ca8d6523e5236c0b9ff8432177d31c3</citedby><cites>FETCH-LOGICAL-c404t-34ee11a57b35386ef7fee0575f5022fda5ca8d6523e5236c0b9ff8432177d31c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25266763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lesniak, A</creatorcontrib><creatorcontrib>Kilinc, D</creatorcontrib><creatorcontrib>Rashdan, Suad A</creatorcontrib><creatorcontrib>von Kriegsheim, A</creatorcontrib><creatorcontrib>Ashall, B</creatorcontrib><creatorcontrib>Zerulla, D</creatorcontrib><creatorcontrib>Kolch, W</creatorcontrib><creatorcontrib>Lee, G. U</creatorcontrib><title>In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells</title><title>Faraday discussions</title><addtitle>Faraday Discuss</addtitle><description>Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selected to be 150-200 nm so that they did not enter the cells. The 80 nm-long Au tips of the nanorods were functionalized with heregulin (HRG), and the micron-long Fe portion was coated with a poly(ethylene glycol) monolayer to minimize non-specific interactions. Nanorods functionalized with HRG were found to preferentially bind to MCF7 cells that express high levels of the receptor tyrosine-protein kinase ErbB2/3. Magnetic tweezers measurements were used to characterize the kinetic properties of the bond between the HRG on the rods and ErbB2/3 on the surface of the cells. The strong magnetization of Fe-Au nanorods makes them excellent candidates for
in-vitro
and
in-vivo
imaging, and magnetic therapeutic applications targeting cancer cells in circulation.</description><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Imaging</subject><subject>Iron - chemistry</subject><subject>Kinases</subject><subject>Lab-On-A-Chip Devices</subject><subject>Magnetic Fields</subject><subject>Magnetic properties</subject><subject>Magnetization</subject><subject>MCF-7 Cells</subject><subject>Nanorods</subject><subject>Nanotubes - chemistry</subject><subject>Neuregulin-1 - chemistry</subject><subject>Optical Tweezers</subject><subject>Rods</subject><subject>Synthesis (chemistry)</subject><issn>1359-6640</issn><issn>1364-5498</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9PFDEUB_DGQATRi3dNvRmSan_PzHFZWMGw8aLnSbd9ZUtm27XtYPCvd5ZFvMmhP_LeJ69Nvgi9ZfQTo6L7bKV3lDKmbl-gYya0JEp27cHurjqitaRH6FUpt5RSPXVfoiOuuNaNFsfo7iriu1BzwqWO7h4nj-sacIgVsrE1pLgrrSHDzTiESPwYH6pmCL_B4Y25iVCDJWk77WbA0cSUkyv4V6hrvJwvGmyiw8vzGVmeES4YtjAM5TU69GYo8ObxPEE_Fhff55fk-tuXq_nsmlhJZSVCAjBmVLMSSrQafOMBqGqUV5Rz74yypnVacQHT0pauOu9bKThrGieYFSfo437uNqefI5Tab0LZ_cBESGPpWSuU6rgQ8nk6PSM140pP9HRPbU6lZPD9NoeNyfc9o_0ukn4uF-cPkXyd8PvHueNqA-6J_s1gAh_2IBf71P2Xab91fjLv_mfEH1zKmrA</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Lesniak, A</creator><creator>Kilinc, D</creator><creator>Rashdan, Suad A</creator><creator>von Kriegsheim, A</creator><creator>Ashall, B</creator><creator>Zerulla, D</creator><creator>Kolch, W</creator><creator>Lee, G. U</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20140101</creationdate><title>In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells</title><author>Lesniak, A ; Kilinc, D ; Rashdan, Suad A ; von Kriegsheim, A ; Ashall, B ; Zerulla, D ; Kolch, W ; Lee, G. U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-34ee11a57b35386ef7fee0575f5022fda5ca8d6523e5236c0b9ff8432177d31c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>Imaging</topic><topic>Iron - chemistry</topic><topic>Kinases</topic><topic>Lab-On-A-Chip Devices</topic><topic>Magnetic Fields</topic><topic>Magnetic properties</topic><topic>Magnetization</topic><topic>MCF-7 Cells</topic><topic>Nanorods</topic><topic>Nanotubes - chemistry</topic><topic>Neuregulin-1 - chemistry</topic><topic>Optical Tweezers</topic><topic>Rods</topic><topic>Synthesis (chemistry)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lesniak, A</creatorcontrib><creatorcontrib>Kilinc, D</creatorcontrib><creatorcontrib>Rashdan, Suad A</creatorcontrib><creatorcontrib>von Kriegsheim, A</creatorcontrib><creatorcontrib>Ashall, B</creatorcontrib><creatorcontrib>Zerulla, D</creatorcontrib><creatorcontrib>Kolch, W</creatorcontrib><creatorcontrib>Lee, G. U</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Faraday discussions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lesniak, A</au><au>Kilinc, D</au><au>Rashdan, Suad A</au><au>von Kriegsheim, A</au><au>Ashall, B</au><au>Zerulla, D</au><au>Kolch, W</au><au>Lee, G. U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells</atitle><jtitle>Faraday discussions</jtitle><addtitle>Faraday Discuss</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>175</volume><spage>189</spage><epage>21</epage><pages>189-21</pages><issn>1359-6640</issn><eissn>1364-5498</eissn><abstract>Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selected to be 150-200 nm so that they did not enter the cells. The 80 nm-long Au tips of the nanorods were functionalized with heregulin (HRG), and the micron-long Fe portion was coated with a poly(ethylene glycol) monolayer to minimize non-specific interactions. Nanorods functionalized with HRG were found to preferentially bind to MCF7 cells that express high levels of the receptor tyrosine-protein kinase ErbB2/3. Magnetic tweezers measurements were used to characterize the kinetic properties of the bond between the HRG on the rods and ErbB2/3 on the surface of the cells. The strong magnetization of Fe-Au nanorods makes them excellent candidates for
in-vitro
and
in-vivo
imaging, and magnetic therapeutic applications targeting cancer cells in circulation.</abstract><cop>England</cop><pmid>25266763</pmid><doi>10.1039/c4fd00115j</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-6640 |
| ispartof | Faraday discussions, 2014-01, Vol.175, p.189-21 |
| issn | 1359-6640 1364-5498 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835592334 |
| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Cancer Cell Line, Tumor Gold - chemistry Humans Imaging Iron - chemistry Kinases Lab-On-A-Chip Devices Magnetic Fields Magnetic properties Magnetization MCF-7 Cells Nanorods Nanotubes - chemistry Neuregulin-1 - chemistry Optical Tweezers Rods Synthesis (chemistry) |
| title | In vitro study of the interaction of heregulin-functionalized magnetic-optical nanorods with MCF7 and MDA-MB-231 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T18%3A10%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20study%20of%20the%20interaction%20of%20heregulin-functionalized%20magnetic-optical%20nanorods%20with%20MCF7%20and%20MDA-MB-231%20cells&rft.jtitle=Faraday%20discussions&rft.au=Lesniak,%20A&rft.date=2014-01-01&rft.volume=175&rft.spage=189&rft.epage=21&rft.pages=189-21&rft.issn=1359-6640&rft.eissn=1364-5498&rft_id=info:doi/10.1039/c4fd00115j&rft_dat=%3Cproquest_cross%3E1835592334%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1652461256&rft_id=info:pmid/25266763&rfr_iscdi=true |