A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells
Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological role...
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| Veröffentlicht in: | Biosensors & bioelectronics 2016-12, Vol.86, p.68-74 |
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| creator | Tian, Fengshou Jia, Yan Zhang, Yanan Song, Wei Zhao, Guangjiu Qu, Zongjin Li, Chunyan Chen, Yahong Li, Peng |
| description | Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological roles under complex biological conditions. In this study, a turn-on near infrared (NIR) fluorescent probe Cy7-NphS has been designed and developed for highly selective and sensitive sensing of HClO and Myeloperoxidase (MPO) with fast response time. The newly developed probe has been successfully applied in real-time monitoring of HClO and MPO activity in PBS solutions and living HL-60 cells. When applied in MPO activity determination, the probe showed very high sensitivity with a detection limit of as low as 3.69×10−3U/mL. Furthermore, the living cell imaging study suggested that this probe could detect HClO in mitochondria.
•Cy7-NphS is a near-infrared probe for HClO/MPO sensing.•Cy7-NphS merit fast response time and high sensitivity.•Cy7-NphS could be used for real-time HClO imaging in living cells.•Cy7-NphS could respond to MPO activity linearly within the clinical range.•Cy7-NphS was mitochondria targeted. |
| doi_str_mv | 10.1016/j.bios.2016.06.039 |
| format | Article |
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•Cy7-NphS is a near-infrared probe for HClO/MPO sensing.•Cy7-NphS merit fast response time and high sensitivity.•Cy7-NphS could be used for real-time HClO imaging in living cells.•Cy7-NphS could respond to MPO activity linearly within the clinical range.•Cy7-NphS was mitochondria targeted.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2016.06.039</identifier><identifier>PMID: 27322938</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bio-imaging ; Bioelectricity ; Biotechnology ; Carbocyanines - chemistry ; Cells (biology) ; ClO−/MPO detection ; Enzyme Activation ; Fluorescence ; Fluorescent Dyes - chemistry ; HL-60 Cells ; Humans ; Hypochlorous Acid - metabolism ; Imaging ; Infrared Rays ; Microscopy, Fluorescence - methods ; Mitochondria ; Mitochondria - enzymology ; Mitochondria - ultrastructure ; Mitochondria targeting ; Molecular Imaging - methods ; Monitoring ; Near-infrared ; Pathogens ; Peroxidase - metabolism ; Real-time monitoring ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Biosensors & bioelectronics, 2016-12, Vol.86, p.68-74</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-88fa6082e65a01b4045fc0e565f89c3f28cb7b7183f0a54acc3264d0511cc3723</citedby><cites>FETCH-LOGICAL-c422t-88fa6082e65a01b4045fc0e565f89c3f28cb7b7183f0a54acc3264d0511cc3723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2016.06.039$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27322938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Fengshou</creatorcontrib><creatorcontrib>Jia, Yan</creatorcontrib><creatorcontrib>Zhang, Yanan</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Zhao, Guangjiu</creatorcontrib><creatorcontrib>Qu, Zongjin</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><creatorcontrib>Chen, Yahong</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><title>A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological roles under complex biological conditions. In this study, a turn-on near infrared (NIR) fluorescent probe Cy7-NphS has been designed and developed for highly selective and sensitive sensing of HClO and Myeloperoxidase (MPO) with fast response time. The newly developed probe has been successfully applied in real-time monitoring of HClO and MPO activity in PBS solutions and living HL-60 cells. When applied in MPO activity determination, the probe showed very high sensitivity with a detection limit of as low as 3.69×10−3U/mL. Furthermore, the living cell imaging study suggested that this probe could detect HClO in mitochondria.
•Cy7-NphS is a near-infrared probe for HClO/MPO sensing.•Cy7-NphS merit fast response time and high sensitivity.•Cy7-NphS could be used for real-time HClO imaging in living cells.•Cy7-NphS could respond to MPO activity linearly within the clinical range.•Cy7-NphS was mitochondria targeted.</description><subject>Bio-imaging</subject><subject>Bioelectricity</subject><subject>Biotechnology</subject><subject>Carbocyanines - chemistry</subject><subject>Cells (biology)</subject><subject>ClO−/MPO detection</subject><subject>Enzyme Activation</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Hypochlorous Acid - metabolism</subject><subject>Imaging</subject><subject>Infrared Rays</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Mitochondria</subject><subject>Mitochondria - enzymology</subject><subject>Mitochondria - ultrastructure</subject><subject>Mitochondria targeting</subject><subject>Molecular Imaging - methods</subject><subject>Monitoring</subject><subject>Near-infrared</subject><subject>Pathogens</subject><subject>Peroxidase - metabolism</subject><subject>Real-time monitoring</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9uEzEQxi0EomngBTggH7ls8J_1elfiUkVAkYp6gbPl9Y7LRLt2sJ2KvAMPjdMUjqjSSB7Jv-8bez5C3nC24Yx373ebEWPeiNpvWC05PCMr3mvZtEKq52TFBtU1quvkBbnMeccY03xgL8mF0FKIQfYr8vuKXm_n2ybvwaFHRwPY1GDwySaYqJ8PMUF2EArdpzgC9THRCQqkBYMtGAONnn49whz3kOIvnGwGal3BeyxHasNEcbF3GO7ogiW6HzFMCe38MJVioHMF66WDec6vyAtv5wyvH881-f7p47ftdXNz-_nL9uqmca0Qpel7bzvWC-iUZXxsWau8Y6A65fvBSS96N-pR8156ZlVrnZOiayemOK-tFnJN3p1965d-HiAXs2A-vcAGiIdsqlIpLZRST0CFHphu5ZNQrgcua0BrIs6oSzHnBN7sU11TOhrOzClbszOnbM0pW8NqyaGK3j76H8YFpn-Sv2FW4MMZgLq7e4RkskMIDiZM4IqZIv7P_w-b97Zz</recordid><startdate>20161215</startdate><enddate>20161215</enddate><creator>Tian, Fengshou</creator><creator>Jia, Yan</creator><creator>Zhang, Yanan</creator><creator>Song, Wei</creator><creator>Zhao, Guangjiu</creator><creator>Qu, Zongjin</creator><creator>Li, Chunyan</creator><creator>Chen, Yahong</creator><creator>Li, Peng</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20161215</creationdate><title>A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells</title><author>Tian, Fengshou ; Jia, Yan ; Zhang, Yanan ; Song, Wei ; Zhao, Guangjiu ; Qu, Zongjin ; Li, Chunyan ; Chen, Yahong ; Li, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-88fa6082e65a01b4045fc0e565f89c3f28cb7b7183f0a54acc3264d0511cc3723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bio-imaging</topic><topic>Bioelectricity</topic><topic>Biotechnology</topic><topic>Carbocyanines - chemistry</topic><topic>Cells (biology)</topic><topic>ClO−/MPO detection</topic><topic>Enzyme Activation</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Hypochlorous Acid - metabolism</topic><topic>Imaging</topic><topic>Infrared Rays</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Mitochondria</topic><topic>Mitochondria - enzymology</topic><topic>Mitochondria - ultrastructure</topic><topic>Mitochondria targeting</topic><topic>Molecular Imaging - methods</topic><topic>Monitoring</topic><topic>Near-infrared</topic><topic>Pathogens</topic><topic>Peroxidase - metabolism</topic><topic>Real-time monitoring</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Fengshou</creatorcontrib><creatorcontrib>Jia, Yan</creatorcontrib><creatorcontrib>Zhang, Yanan</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Zhao, Guangjiu</creatorcontrib><creatorcontrib>Qu, Zongjin</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><creatorcontrib>Chen, Yahong</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Fengshou</au><au>Jia, Yan</au><au>Zhang, Yanan</au><au>Song, Wei</au><au>Zhao, Guangjiu</au><au>Qu, Zongjin</au><au>Li, Chunyan</au><au>Chen, Yahong</au><au>Li, Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2016-12-15</date><risdate>2016</risdate><volume>86</volume><spage>68</spage><epage>74</epage><pages>68-74</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological roles under complex biological conditions. In this study, a turn-on near infrared (NIR) fluorescent probe Cy7-NphS has been designed and developed for highly selective and sensitive sensing of HClO and Myeloperoxidase (MPO) with fast response time. The newly developed probe has been successfully applied in real-time monitoring of HClO and MPO activity in PBS solutions and living HL-60 cells. When applied in MPO activity determination, the probe showed very high sensitivity with a detection limit of as low as 3.69×10−3U/mL. Furthermore, the living cell imaging study suggested that this probe could detect HClO in mitochondria.
•Cy7-NphS is a near-infrared probe for HClO/MPO sensing.•Cy7-NphS merit fast response time and high sensitivity.•Cy7-NphS could be used for real-time HClO imaging in living cells.•Cy7-NphS could respond to MPO activity linearly within the clinical range.•Cy7-NphS was mitochondria targeted.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>27322938</pmid><doi>10.1016/j.bios.2016.06.039</doi><tpages>7</tpages></addata></record> |
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| subjects | Bio-imaging Bioelectricity Biotechnology Carbocyanines - chemistry Cells (biology) ClO−/MPO detection Enzyme Activation Fluorescence Fluorescent Dyes - chemistry HL-60 Cells Humans Hypochlorous Acid - metabolism Imaging Infrared Rays Microscopy, Fluorescence - methods Mitochondria Mitochondria - enzymology Mitochondria - ultrastructure Mitochondria targeting Molecular Imaging - methods Monitoring Near-infrared Pathogens Peroxidase - metabolism Real-time monitoring Reproducibility of Results Sensitivity and Specificity |
| title | A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells |
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