3D Printed Micro Free-Flow Electrophoresis Device
The cost, time, and restrictions on creative flexibility associated with current fabrication methods present significant challenges in the development and application of microfluidic devices. Additive manufacturing, also referred to as three-dimensional (3D) printing, provides many advantages over e...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2016-08, Vol.88 (15), p.7675-7682 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7682 |
|---|---|
| container_issue | 15 |
| container_start_page | 7675 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 88 |
| creator | Anciaux, Sarah K. Geiger, Matthew Bowser, Michael T. |
| description | The cost, time, and restrictions on creative flexibility associated with current fabrication methods present significant challenges in the development and application of microfluidic devices. Additive manufacturing, also referred to as three-dimensional (3D) printing, provides many advantages over existing methods. With 3D printing, devices can be made in a cost-effective manner with the ability to rapidly prototype new designs. We have fabricated a micro free-flow electrophoresis (μFFE) device using a low-cost, consumer-grade 3D printer. Test prints were performed to determine the minimum feature sizes that could be reproducibly produced using 3D printing fabrication. Microfluidic ridges could be fabricated with dimensions as small as 20 μm high × 640 μm wide. Minimum valley dimensions were 30 μm wide × 130 μm wide. An acetone vapor bath was used to smooth acrylonitrile–butadiene–styrene (ABS) surfaces and facilitate bonding of fully enclosed channels. The surfaces of the 3D-printed features were profiled and compared to a similar device fabricated in a glass substrate. Stable stream profiles were obtained in a 3D-printed μFFE device. Separations of fluorescent dyes in the 3D-printed device and its glass counterpart were comparable. A μFFE separation of myoglobin and cytochrome c was also demonstrated on a 3D-printed device. Limits of detection for rhodamine 110 were determined to be 2 and 0.3 nM for the 3D-printed and glass devices, respectively. |
| doi_str_mv | 10.1021/acs.analchem.6b01573 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835560279</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4147148021</sourcerecordid><originalsourceid>FETCH-LOGICAL-a516t-671228f2cb4184269029c2118949783a1819a6c1643fd40140c791aa33899f23</originalsourceid><addsrcrecordid>eNqNkEtLAzEURoMotlb_gciAGzdT700yeSylDxUUXXQf0jRDp8x0ajKj-O-d0lbBhbi6m_OdC4eQS4QhAsVb6-LQrm3plr4aijlgJtkR6WNGIRVK0WPSBwCWUgnQI2cxrgAQAcUp6VHJpGQZ7xNk4-Q1FOvGL5LnwoU6mQbv02lZfyST0rsm1JtlHXwsYjL274Xz5-Qkt2X0F_s7ILPpZDZ6SJ9e7h9Hd0-pzVA0qZBIqcqpm3NUnAoNVDuKqDTXUjGLCrUVDgVn-YIDcnBSo7WMKa1zygbkZqfdhPqt9bExVRGdL0u79nUbDSqWZQKo1P9AQQngGRcdev0LXdVt6CpuKUSqM5TQUXxHdT1iDD43m1BUNnwaBLONb7r45hDf7ON3s6u9vJ1XfvE9OtTuANgB2_nP47-cX408jl4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1811295170</pqid></control><display><type>article</type><title>3D Printed Micro Free-Flow Electrophoresis Device</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Anciaux, Sarah K. ; Geiger, Matthew ; Bowser, Michael T.</creator><creatorcontrib>Anciaux, Sarah K. ; Geiger, Matthew ; Bowser, Michael T.</creatorcontrib><description>The cost, time, and restrictions on creative flexibility associated with current fabrication methods present significant challenges in the development and application of microfluidic devices. Additive manufacturing, also referred to as three-dimensional (3D) printing, provides many advantages over existing methods. With 3D printing, devices can be made in a cost-effective manner with the ability to rapidly prototype new designs. We have fabricated a micro free-flow electrophoresis (μFFE) device using a low-cost, consumer-grade 3D printer. Test prints were performed to determine the minimum feature sizes that could be reproducibly produced using 3D printing fabrication. Microfluidic ridges could be fabricated with dimensions as small as 20 μm high × 640 μm wide. Minimum valley dimensions were 30 μm wide × 130 μm wide. An acetone vapor bath was used to smooth acrylonitrile–butadiene–styrene (ABS) surfaces and facilitate bonding of fully enclosed channels. The surfaces of the 3D-printed features were profiled and compared to a similar device fabricated in a glass substrate. Stable stream profiles were obtained in a 3D-printed μFFE device. Separations of fluorescent dyes in the 3D-printed device and its glass counterpart were comparable. A μFFE separation of myoglobin and cytochrome c was also demonstrated on a 3D-printed device. Limits of detection for rhodamine 110 were determined to be 2 and 0.3 nM for the 3D-printed and glass devices, respectively.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.6b01573</identifier><identifier>PMID: 27377354</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>3-D printers ; 3D printing ; ABS resins ; Additive manufacturing ; Analytical chemistry ; Capillary electrophoresis ; Chemical bonds ; Cytochromes c - isolation & purification ; Devices ; Electrophoresis ; Electrophoresis - economics ; Electrophoresis - instrumentation ; Electrophoresis - methods ; Glass ; Glass substrates ; Limit of Detection ; Microfluidics ; Myoglobin - isolation & purification ; Printing, Three-Dimensional - instrumentation ; Rapid prototyping ; Rhodamines - analysis ; Separation ; Three dimensional printing</subject><ispartof>Analytical chemistry (Washington), 2016-08, Vol.88 (15), p.7675-7682</ispartof><rights>Copyright © 2016 American Chemical Society</rights><rights>Copyright American Chemical Society Aug 2, 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a516t-671228f2cb4184269029c2118949783a1819a6c1643fd40140c791aa33899f23</citedby><cites>FETCH-LOGICAL-a516t-671228f2cb4184269029c2118949783a1819a6c1643fd40140c791aa33899f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.6b01573$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.6b01573$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27377354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anciaux, Sarah K.</creatorcontrib><creatorcontrib>Geiger, Matthew</creatorcontrib><creatorcontrib>Bowser, Michael T.</creatorcontrib><title>3D Printed Micro Free-Flow Electrophoresis Device</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The cost, time, and restrictions on creative flexibility associated with current fabrication methods present significant challenges in the development and application of microfluidic devices. Additive manufacturing, also referred to as three-dimensional (3D) printing, provides many advantages over existing methods. With 3D printing, devices can be made in a cost-effective manner with the ability to rapidly prototype new designs. We have fabricated a micro free-flow electrophoresis (μFFE) device using a low-cost, consumer-grade 3D printer. Test prints were performed to determine the minimum feature sizes that could be reproducibly produced using 3D printing fabrication. Microfluidic ridges could be fabricated with dimensions as small as 20 μm high × 640 μm wide. Minimum valley dimensions were 30 μm wide × 130 μm wide. An acetone vapor bath was used to smooth acrylonitrile–butadiene–styrene (ABS) surfaces and facilitate bonding of fully enclosed channels. The surfaces of the 3D-printed features were profiled and compared to a similar device fabricated in a glass substrate. Stable stream profiles were obtained in a 3D-printed μFFE device. Separations of fluorescent dyes in the 3D-printed device and its glass counterpart were comparable. A μFFE separation of myoglobin and cytochrome c was also demonstrated on a 3D-printed device. Limits of detection for rhodamine 110 were determined to be 2 and 0.3 nM for the 3D-printed and glass devices, respectively.</description><subject>3-D printers</subject><subject>3D printing</subject><subject>ABS resins</subject><subject>Additive manufacturing</subject><subject>Analytical chemistry</subject><subject>Capillary electrophoresis</subject><subject>Chemical bonds</subject><subject>Cytochromes c - isolation & purification</subject><subject>Devices</subject><subject>Electrophoresis</subject><subject>Electrophoresis - economics</subject><subject>Electrophoresis - instrumentation</subject><subject>Electrophoresis - methods</subject><subject>Glass</subject><subject>Glass substrates</subject><subject>Limit of Detection</subject><subject>Microfluidics</subject><subject>Myoglobin - isolation & purification</subject><subject>Printing, Three-Dimensional - instrumentation</subject><subject>Rapid prototyping</subject><subject>Rhodamines - analysis</subject><subject>Separation</subject><subject>Three dimensional printing</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLAzEURoMotlb_gciAGzdT700yeSylDxUUXXQf0jRDp8x0ajKj-O-d0lbBhbi6m_OdC4eQS4QhAsVb6-LQrm3plr4aijlgJtkR6WNGIRVK0WPSBwCWUgnQI2cxrgAQAcUp6VHJpGQZ7xNk4-Q1FOvGL5LnwoU6mQbv02lZfyST0rsm1JtlHXwsYjL274Xz5-Qkt2X0F_s7ILPpZDZ6SJ9e7h9Hd0-pzVA0qZBIqcqpm3NUnAoNVDuKqDTXUjGLCrUVDgVn-YIDcnBSo7WMKa1zygbkZqfdhPqt9bExVRGdL0u79nUbDSqWZQKo1P9AQQngGRcdev0LXdVt6CpuKUSqM5TQUXxHdT1iDD43m1BUNnwaBLONb7r45hDf7ON3s6u9vJ1XfvE9OtTuANgB2_nP47-cX408jl4</recordid><startdate>20160802</startdate><enddate>20160802</enddate><creator>Anciaux, Sarah K.</creator><creator>Geiger, Matthew</creator><creator>Bowser, Michael T.</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20160802</creationdate><title>3D Printed Micro Free-Flow Electrophoresis Device</title><author>Anciaux, Sarah K. ; Geiger, Matthew ; Bowser, Michael T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a516t-671228f2cb4184269029c2118949783a1819a6c1643fd40140c791aa33899f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3-D printers</topic><topic>3D printing</topic><topic>ABS resins</topic><topic>Additive manufacturing</topic><topic>Analytical chemistry</topic><topic>Capillary electrophoresis</topic><topic>Chemical bonds</topic><topic>Cytochromes c - isolation & purification</topic><topic>Devices</topic><topic>Electrophoresis</topic><topic>Electrophoresis - economics</topic><topic>Electrophoresis - instrumentation</topic><topic>Electrophoresis - methods</topic><topic>Glass</topic><topic>Glass substrates</topic><topic>Limit of Detection</topic><topic>Microfluidics</topic><topic>Myoglobin - isolation & purification</topic><topic>Printing, Three-Dimensional - instrumentation</topic><topic>Rapid prototyping</topic><topic>Rhodamines - analysis</topic><topic>Separation</topic><topic>Three dimensional printing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anciaux, Sarah K.</creatorcontrib><creatorcontrib>Geiger, Matthew</creatorcontrib><creatorcontrib>Bowser, Michael T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anciaux, Sarah K.</au><au>Geiger, Matthew</au><au>Bowser, Michael T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D Printed Micro Free-Flow Electrophoresis Device</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2016-08-02</date><risdate>2016</risdate><volume>88</volume><issue>15</issue><spage>7675</spage><epage>7682</epage><pages>7675-7682</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The cost, time, and restrictions on creative flexibility associated with current fabrication methods present significant challenges in the development and application of microfluidic devices. Additive manufacturing, also referred to as three-dimensional (3D) printing, provides many advantages over existing methods. With 3D printing, devices can be made in a cost-effective manner with the ability to rapidly prototype new designs. We have fabricated a micro free-flow electrophoresis (μFFE) device using a low-cost, consumer-grade 3D printer. Test prints were performed to determine the minimum feature sizes that could be reproducibly produced using 3D printing fabrication. Microfluidic ridges could be fabricated with dimensions as small as 20 μm high × 640 μm wide. Minimum valley dimensions were 30 μm wide × 130 μm wide. An acetone vapor bath was used to smooth acrylonitrile–butadiene–styrene (ABS) surfaces and facilitate bonding of fully enclosed channels. The surfaces of the 3D-printed features were profiled and compared to a similar device fabricated in a glass substrate. Stable stream profiles were obtained in a 3D-printed μFFE device. Separations of fluorescent dyes in the 3D-printed device and its glass counterpart were comparable. A μFFE separation of myoglobin and cytochrome c was also demonstrated on a 3D-printed device. Limits of detection for rhodamine 110 were determined to be 2 and 0.3 nM for the 3D-printed and glass devices, respectively.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>27377354</pmid><doi>10.1021/acs.analchem.6b01573</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2016-08, Vol.88 (15), p.7675-7682 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835560279 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | 3-D printers 3D printing ABS resins Additive manufacturing Analytical chemistry Capillary electrophoresis Chemical bonds Cytochromes c - isolation & purification Devices Electrophoresis Electrophoresis - economics Electrophoresis - instrumentation Electrophoresis - methods Glass Glass substrates Limit of Detection Microfluidics Myoglobin - isolation & purification Printing, Three-Dimensional - instrumentation Rapid prototyping Rhodamines - analysis Separation Three dimensional printing |
| title | 3D Printed Micro Free-Flow Electrophoresis Device |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T05%3A26%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=3D%20Printed%20Micro%20Free-Flow%20Electrophoresis%20Device&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Anciaux,%20Sarah%20K.&rft.date=2016-08-02&rft.volume=88&rft.issue=15&rft.spage=7675&rft.epage=7682&rft.pages=7675-7682&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/acs.analchem.6b01573&rft_dat=%3Cproquest_cross%3E4147148021%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1811295170&rft_id=info:pmid/27377354&rfr_iscdi=true |