Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs
Summary The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal v...
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| Veröffentlicht in: | Journal of animal physiology and animal nutrition 2017-10, Vol.101 (5), p.e414-e426 |
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| creator | Liermann, W. Berk, A. Frahm, J. Böschen, V. Dänicke, S. |
| description | Summary
The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 = normal to 4 = severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T‐cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4+, CD8+, CD8low, CD4/CD8 double‐positive T cells, the mean fluorescence intensity of CD4+ T cells and the ratio of CD8low/CD8high T cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1–2.5) and animals with high MSC (MSC = 3–4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0–0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail. |
| doi_str_mv | 10.1111/jpn.12623 |
| format | Article |
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The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 = normal to 4 = severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T‐cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4+, CD8+, CD8low, CD4/CD8 double‐positive T cells, the mean fluorescence intensity of CD4+ T cells and the ratio of CD8low/CD8high T cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1–2.5) and animals with high MSC (MSC = 3–4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0–0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail.</description><identifier>ISSN: 0931-2439</identifier><identifier>EISSN: 1439-0396</identifier><identifier>DOI: 10.1111/jpn.12623</identifier><identifier>PMID: 27805297</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Adaptive Immunity ; Animal Feed ; Animals ; Beans ; Blood ; CD4 antigen ; CD4+ cells ; CD8 antigen ; CD8+ cells ; Diet ; Diet - veterinary ; Feeding ; Feeds ; Flow cytometry ; Fluorescence ; Food Handling ; Gastrointestinal Contents ; Immune system ; Immunology ; intestinal immune system ; Intestine ; Lamina propria ; Lesions ; Lymph nodes ; Lymphocytes ; Lymphocytes T ; Male ; Monoclonal antibodies ; Pelleted feeds ; pelleting ; Peyer's patches ; Phenotyping ; Pigs ; Rapeseed ; Soybeans ; Stomach ; Stomach - immunology ; Stomach Diseases - pathology ; Stomach Diseases - prevention & control ; Stomach Diseases - veterinary ; Swine - immunology ; Swine - physiology ; Swine Diseases - prevention & control ; T-Lymphocyte Subsets - physiology ; Tissues</subject><ispartof>Journal of animal physiology and animal nutrition, 2017-10, Vol.101 (5), p.e414-e426</ispartof><rights>Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH</rights><rights>Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.</rights><rights>2017 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-bf8c645117f9519f73965794f1a8092eef93743b61a484ed240e13fefe65773f3</citedby><cites>FETCH-LOGICAL-c3533-bf8c645117f9519f73965794f1a8092eef93743b61a484ed240e13fefe65773f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpn.12623$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpn.12623$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27805297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liermann, W.</creatorcontrib><creatorcontrib>Berk, A.</creatorcontrib><creatorcontrib>Frahm, J.</creatorcontrib><creatorcontrib>Böschen, V.</creatorcontrib><creatorcontrib>Dänicke, S.</creatorcontrib><title>Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs</title><title>Journal of animal physiology and animal nutrition</title><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><description>Summary
The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 = normal to 4 = severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T‐cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4+, CD8+, CD8low, CD4/CD8 double‐positive T cells, the mean fluorescence intensity of CD4+ T cells and the ratio of CD8low/CD8high T cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1–2.5) and animals with high MSC (MSC = 3–4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0–0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail.</description><subject>Adaptive Immunity</subject><subject>Animal Feed</subject><subject>Animals</subject><subject>Beans</subject><subject>Blood</subject><subject>CD4 antigen</subject><subject>CD4+ cells</subject><subject>CD8 antigen</subject><subject>CD8+ cells</subject><subject>Diet</subject><subject>Diet - veterinary</subject><subject>Feeding</subject><subject>Feeds</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Food Handling</subject><subject>Gastrointestinal Contents</subject><subject>Immune system</subject><subject>Immunology</subject><subject>intestinal immune system</subject><subject>Intestine</subject><subject>Lamina propria</subject><subject>Lesions</subject><subject>Lymph nodes</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Monoclonal antibodies</subject><subject>Pelleted feeds</subject><subject>pelleting</subject><subject>Peyer's patches</subject><subject>Phenotyping</subject><subject>Pigs</subject><subject>Rapeseed</subject><subject>Soybeans</subject><subject>Stomach</subject><subject>Stomach - immunology</subject><subject>Stomach Diseases - pathology</subject><subject>Stomach Diseases - prevention & control</subject><subject>Stomach Diseases - veterinary</subject><subject>Swine - immunology</subject><subject>Swine - physiology</subject><subject>Swine Diseases - prevention & control</subject><subject>T-Lymphocyte Subsets - physiology</subject><subject>Tissues</subject><issn>0931-2439</issn><issn>1439-0396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9PHCEYh0lT427VQ79AQ9JLPczKn2FmODZma2uMetAzYdkXZTPAdmCy2W8v69geTORC-OXhyQs_hL5SsqBlXWy2YUFZw_gnNKc1lxXhsvmM5kRyWrESzNCXlDaE0FaQ5hjNWNsRwWQ7R2lpLZiccLQ47yI20XsYjNM9XjsouQ5rnME8B2dKZgHKcQCdPYSMY8ApR6_NM-4huRgm3nk_BsBpnzL4g9nqnCG48IS37imdoiOr-wRnb_sJevy1fLj8Xd3cXf25_HlTGS44r1a2M00tKG2tFFTatjxKtLK2VHdEMgAreVvzVUN13dWwZjUByi1YKFjLLT9BPybvdoh_R0hZeZcM9L0OEMekaMeFKLc4Kej3d-gmjkMo0ykqeSdY17EDdT5RZogpDWDVdnBeD3tFiTo0oUoT6rWJwn57M44rD-v_5L-vL8DFBOxcD_uPTer6_nZSvgC3cJI3</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Liermann, W.</creator><creator>Berk, A.</creator><creator>Frahm, J.</creator><creator>Böschen, V.</creator><creator>Dänicke, S.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201710</creationdate><title>Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs</title><author>Liermann, W. ; Berk, A. ; Frahm, J. ; Böschen, V. ; Dänicke, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-bf8c645117f9519f73965794f1a8092eef93743b61a484ed240e13fefe65773f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adaptive Immunity</topic><topic>Animal Feed</topic><topic>Animals</topic><topic>Beans</topic><topic>Blood</topic><topic>CD4 antigen</topic><topic>CD4+ cells</topic><topic>CD8 antigen</topic><topic>CD8+ cells</topic><topic>Diet</topic><topic>Diet - veterinary</topic><topic>Feeding</topic><topic>Feeds</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>Food Handling</topic><topic>Gastrointestinal Contents</topic><topic>Immune system</topic><topic>Immunology</topic><topic>intestinal immune system</topic><topic>Intestine</topic><topic>Lamina propria</topic><topic>Lesions</topic><topic>Lymph nodes</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Monoclonal antibodies</topic><topic>Pelleted feeds</topic><topic>pelleting</topic><topic>Peyer's patches</topic><topic>Phenotyping</topic><topic>Pigs</topic><topic>Rapeseed</topic><topic>Soybeans</topic><topic>Stomach</topic><topic>Stomach - immunology</topic><topic>Stomach Diseases - pathology</topic><topic>Stomach Diseases - prevention & control</topic><topic>Stomach Diseases - veterinary</topic><topic>Swine - immunology</topic><topic>Swine - physiology</topic><topic>Swine Diseases - prevention & control</topic><topic>T-Lymphocyte Subsets - physiology</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liermann, W.</creatorcontrib><creatorcontrib>Berk, A.</creatorcontrib><creatorcontrib>Frahm, J.</creatorcontrib><creatorcontrib>Böschen, V.</creatorcontrib><creatorcontrib>Dänicke, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal physiology and animal nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liermann, W.</au><au>Berk, A.</au><au>Frahm, J.</au><au>Böschen, V.</au><au>Dänicke, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs</atitle><jtitle>Journal of animal physiology and animal nutrition</jtitle><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><date>2017-10</date><risdate>2017</risdate><volume>101</volume><issue>5</issue><spage>e414</spage><epage>e426</epage><pages>e414-e426</pages><issn>0931-2439</issn><eissn>1439-0396</eissn><abstract>Summary
The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 = normal to 4 = severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T‐cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4+, CD8+, CD8low, CD4/CD8 double‐positive T cells, the mean fluorescence intensity of CD4+ T cells and the ratio of CD8low/CD8high T cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1–2.5) and animals with high MSC (MSC = 3–4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0–0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27805297</pmid><doi>10.1111/jpn.12623</doi><tpages>13</tpages></addata></record> |
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| subjects | Adaptive Immunity Animal Feed Animals Beans Blood CD4 antigen CD4+ cells CD8 antigen CD8+ cells Diet Diet - veterinary Feeding Feeds Flow cytometry Fluorescence Food Handling Gastrointestinal Contents Immune system Immunology intestinal immune system Intestine Lamina propria Lesions Lymph nodes Lymphocytes Lymphocytes T Male Monoclonal antibodies Pelleted feeds pelleting Peyer's patches Phenotyping Pigs Rapeseed Soybeans Stomach Stomach - immunology Stomach Diseases - pathology Stomach Diseases - prevention & control Stomach Diseases - veterinary Swine - immunology Swine - physiology Swine Diseases - prevention & control T-Lymphocyte Subsets - physiology Tissues |
| title | Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs |
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