Biocompatibility of three new calcium silicate‐based endodontic sealers on human periodontal ligament stem cells

Aim To evaluate the biocompatibility of three calcium silicate‐based endodontic sealers, Bioroot BC Sealer (Septodont, Saint‐Maur‐des‐Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano‐ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs). Methodology Human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound‐healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One‐way analysis of variance (anova) followed by a Bonferroni post‐test was performed (P