Transient fusion ensures granule replenishment to enable repeated release after IgE-mediated mast cell degranulation
To ensure normal immune function, mast cells employ different pathways to release mediators. Here, we report a thus far unknown capacity of mast cells to recycle and reuse secretory granules after an antigen-evoked degranulation process under physiological conditions; this phenomenon involves the ex...
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| Veröffentlicht in: | Journal of cell science 2016-11, Vol.129 (21), p.3989-4000 |
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| creator | Balseiro-Gomez, Santiago Flores, Juan A Acosta, Jorge Ramirez-Ponce, M Pilar Ales, Eva |
| description | To ensure normal immune function, mast cells employ different pathways to release mediators. Here, we report a thus far unknown capacity of mast cells to recycle and reuse secretory granules after an antigen-evoked degranulation process under physiological conditions; this phenomenon involves the existence of a recycling secretory granule pool that is available for release in a short time scale. Rapid endocytic modes contributed to the recycling of ∼60% of the total secretory granule population, which involved kiss-and-run and cavicapture mechanisms, causing retention of the intragranular matrix. We found the presence of normal-size granules and giant actomyosin- and dynamin-dependent granules, which were characterized by large quantal content. These large structures allowed the recovered mast cells to release a large amount of 5-HT, compensating for the decrease in the number of exocytosed secretory granules. This work uncovers a new physiological role of the exo-endocytosis cycle in the immunological plasticity of mast cells and reveals a new property of their biological secretion. |
| doi_str_mv | 10.1242/jcs.194340 |
| format | Article |
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Here, we report a thus far unknown capacity of mast cells to recycle and reuse secretory granules after an antigen-evoked degranulation process under physiological conditions; this phenomenon involves the existence of a recycling secretory granule pool that is available for release in a short time scale. Rapid endocytic modes contributed to the recycling of ∼60% of the total secretory granule population, which involved kiss-and-run and cavicapture mechanisms, causing retention of the intragranular matrix. We found the presence of normal-size granules and giant actomyosin- and dynamin-dependent granules, which were characterized by large quantal content. These large structures allowed the recovered mast cells to release a large amount of 5-HT, compensating for the decrease in the number of exocytosed secretory granules. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Actins - metabolism Animals Antigens - metabolism Calcimycin - pharmacology Cell Degranulation - drug effects Dynamins - metabolism Electrochemical Techniques Endocytosis - drug effects Exocytosis - drug effects Immunoglobulin E - metabolism Mast Cells - drug effects Mast Cells - physiology Membrane Fusion - drug effects Mice, Inbred C57BL Models, Biological Myosin Type II - metabolism Secretory Vesicles - drug effects Secretory Vesicles - metabolism Serotonin - metabolism |
| title | Transient fusion ensures granule replenishment to enable repeated release after IgE-mediated mast cell degranulation |
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