A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery
It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective t...
Gespeichert in:
| Veröffentlicht in: | Materials Science & Engineering C 2017-01, Vol.70 (Pt 1), p.572-585 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 585 |
|---|---|
| container_issue | Pt 1 |
| container_start_page | 572 |
| container_title | Materials Science & Engineering C |
| container_volume | 70 |
| creator | Gu, Yaming Guo, Yizhen Wang, Changyong Xu, Jiake Wu, Jianping Kirk, Thomas Brett Ma, Dong Xue, Wei |
| description | It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
[Display omitted]
•PAMAM-functionalized GO was prepared and used to co-deliver DOX and MMP-9 shRNA plasmid.•It showed an effective delivery of DOX and MMP-9 to MCF-7 cells.•The co-loaded PAMAM-GO suggested a potential application in cancer therapy. |
| doi_str_mv | 10.1016/j.msec.2016.09.035 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835521142</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0928493116313686</els_id><sourcerecordid>1835521142</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-707d7df2dd414c6e8e75f8dcdc2d581ca0579d0fa46e63718dd0f8cf4419cbc33</originalsourceid><addsrcrecordid>eNp9kE1v1DAQQC0EotvCH-CAfOSS4I8ktiUuVYGC1FKEQOJmuTMT6lUSL_ZuxfLr8WoLR04eS2-eNI-xF1K0Usjh9bqdC0Gr6twK1wrdP2IraY1uhHTyMVsJp2zTOS1P2GkpayEGq416yk6UMUY4LVYMzvkmTfswR0xhXogjLZjjTJmPuwW2MS1hir8J-Y8cNndUifQrIvExZf725jsPC_Lr68-N4-Xuy6dqm0KpMg6pQZriPeX9M_ZkDFOh5w_vGfv2_t3Xiw_N1c3lx4vzqwZ0P2wbIwwaHBViJzsYyJLpR4uAoLC3EoLojUMxhm6gQRtpsX4sjF0nHdyC1mfs1dG7yennjsrWz7EATVNYKO2Kl1b3vZKyUxVVRxRyKiXT6Df16JD3Xgp_iOvX_hDXH-J64XyNW5dePvh3tzPhv5W_NSvw5ghQvfI-UvYFIi1AGDPB1mOK__P_AdfIi5I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835521142</pqid></control><display><type>article</type><title>A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Gu, Yaming ; Guo, Yizhen ; Wang, Changyong ; Xu, Jiake ; Wu, Jianping ; Kirk, Thomas Brett ; Ma, Dong ; Xue, Wei</creator><creatorcontrib>Gu, Yaming ; Guo, Yizhen ; Wang, Changyong ; Xu, Jiake ; Wu, Jianping ; Kirk, Thomas Brett ; Ma, Dong ; Xue, Wei</creatorcontrib><description>It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
[Display omitted]
•PAMAM-functionalized GO was prepared and used to co-deliver DOX and MMP-9 shRNA plasmid.•It showed an effective delivery of DOX and MMP-9 to MCF-7 cells.•The co-loaded PAMAM-GO suggested a potential application in cancer therapy.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2016.09.035</identifier><identifier>PMID: 27770930</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3T3 Cells ; Animals ; Apoptosis - drug effects ; Biocompatible Materials - pharmacology ; Cell Survival - drug effects ; Co-delivery ; Dendrimer ; Dendrimers - chemical synthesis ; Dendrimers - chemistry ; DNA - metabolism ; DOX ; Doxorubicin - pharmacology ; Drug Delivery Systems ; Drug Liberation ; Electrophoresis, Agar Gel ; Flow Cytometry ; Gene Transfer Techniques ; Graphene oxide ; Graphite - chemistry ; Humans ; Inhibitory Concentration 50 ; Matrix Metalloproteinase 9 - metabolism ; MCF-7 Cells ; Mice ; MMP-9 shRNA plasmid ; Plasmids - administration & dosage ; RNA, Small Interfering - administration & dosage ; Spectroscopy, Fourier Transform Infrared ; Spectrum Analysis, Raman ; Static Electricity ; Thermogravimetry ; Transfection</subject><ispartof>Materials Science & Engineering C, 2017-01, Vol.70 (Pt 1), p.572-585</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-707d7df2dd414c6e8e75f8dcdc2d581ca0579d0fa46e63718dd0f8cf4419cbc33</citedby><cites>FETCH-LOGICAL-c356t-707d7df2dd414c6e8e75f8dcdc2d581ca0579d0fa46e63718dd0f8cf4419cbc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0928493116313686$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27770930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Yaming</creatorcontrib><creatorcontrib>Guo, Yizhen</creatorcontrib><creatorcontrib>Wang, Changyong</creatorcontrib><creatorcontrib>Xu, Jiake</creatorcontrib><creatorcontrib>Wu, Jianping</creatorcontrib><creatorcontrib>Kirk, Thomas Brett</creatorcontrib><creatorcontrib>Ma, Dong</creatorcontrib><creatorcontrib>Xue, Wei</creatorcontrib><title>A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
[Display omitted]
•PAMAM-functionalized GO was prepared and used to co-deliver DOX and MMP-9 shRNA plasmid.•It showed an effective delivery of DOX and MMP-9 to MCF-7 cells.•The co-loaded PAMAM-GO suggested a potential application in cancer therapy.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Co-delivery</subject><subject>Dendrimer</subject><subject>Dendrimers - chemical synthesis</subject><subject>Dendrimers - chemistry</subject><subject>DNA - metabolism</subject><subject>DOX</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Delivery Systems</subject><subject>Drug Liberation</subject><subject>Electrophoresis, Agar Gel</subject><subject>Flow Cytometry</subject><subject>Gene Transfer Techniques</subject><subject>Graphene oxide</subject><subject>Graphite - chemistry</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>MCF-7 Cells</subject><subject>Mice</subject><subject>MMP-9 shRNA plasmid</subject><subject>Plasmids - administration & dosage</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Spectrum Analysis, Raman</subject><subject>Static Electricity</subject><subject>Thermogravimetry</subject><subject>Transfection</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQQC0EotvCH-CAfOSS4I8ktiUuVYGC1FKEQOJmuTMT6lUSL_ZuxfLr8WoLR04eS2-eNI-xF1K0Usjh9bqdC0Gr6twK1wrdP2IraY1uhHTyMVsJp2zTOS1P2GkpayEGq416yk6UMUY4LVYMzvkmTfswR0xhXogjLZjjTJmPuwW2MS1hir8J-Y8cNndUifQrIvExZf725jsPC_Lr68-N4-Xuy6dqm0KpMg6pQZriPeX9M_ZkDFOh5w_vGfv2_t3Xiw_N1c3lx4vzqwZ0P2wbIwwaHBViJzsYyJLpR4uAoLC3EoLojUMxhm6gQRtpsX4sjF0nHdyC1mfs1dG7yennjsrWz7EATVNYKO2Kl1b3vZKyUxVVRxRyKiXT6Df16JD3Xgp_iOvX_hDXH-J64XyNW5dePvh3tzPhv5W_NSvw5ghQvfI-UvYFIi1AGDPB1mOK__P_AdfIi5I</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Gu, Yaming</creator><creator>Guo, Yizhen</creator><creator>Wang, Changyong</creator><creator>Xu, Jiake</creator><creator>Wu, Jianping</creator><creator>Kirk, Thomas Brett</creator><creator>Ma, Dong</creator><creator>Xue, Wei</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery</title><author>Gu, Yaming ; Guo, Yizhen ; Wang, Changyong ; Xu, Jiake ; Wu, Jianping ; Kirk, Thomas Brett ; Ma, Dong ; Xue, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-707d7df2dd414c6e8e75f8dcdc2d581ca0579d0fa46e63718dd0f8cf4419cbc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>Co-delivery</topic><topic>Dendrimer</topic><topic>Dendrimers - chemical synthesis</topic><topic>Dendrimers - chemistry</topic><topic>DNA - metabolism</topic><topic>DOX</topic><topic>Doxorubicin - pharmacology</topic><topic>Drug Delivery Systems</topic><topic>Drug Liberation</topic><topic>Electrophoresis, Agar Gel</topic><topic>Flow Cytometry</topic><topic>Gene Transfer Techniques</topic><topic>Graphene oxide</topic><topic>Graphite - chemistry</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>MCF-7 Cells</topic><topic>Mice</topic><topic>MMP-9 shRNA plasmid</topic><topic>Plasmids - administration & dosage</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Spectrum Analysis, Raman</topic><topic>Static Electricity</topic><topic>Thermogravimetry</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Gu, Yaming</creatorcontrib><creatorcontrib>Guo, Yizhen</creatorcontrib><creatorcontrib>Wang, Changyong</creatorcontrib><creatorcontrib>Xu, Jiake</creatorcontrib><creatorcontrib>Wu, Jianping</creatorcontrib><creatorcontrib>Kirk, Thomas Brett</creatorcontrib><creatorcontrib>Ma, Dong</creatorcontrib><creatorcontrib>Xue, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Materials Science & Engineering C</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Yaming</au><au>Guo, Yizhen</au><au>Wang, Changyong</au><au>Xu, Jiake</au><au>Wu, Jianping</au><au>Kirk, Thomas Brett</au><au>Ma, Dong</au><au>Xue, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>70</volume><issue>Pt 1</issue><spage>572</spage><epage>585</epage><pages>572-585</pages><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
[Display omitted]
•PAMAM-functionalized GO was prepared and used to co-deliver DOX and MMP-9 shRNA plasmid.•It showed an effective delivery of DOX and MMP-9 to MCF-7 cells.•The co-loaded PAMAM-GO suggested a potential application in cancer therapy.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27770930</pmid><doi>10.1016/j.msec.2016.09.035</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0928-4931 |
| ispartof | Materials Science & Engineering C, 2017-01, Vol.70 (Pt 1), p.572-585 |
| issn | 0928-4931 1873-0191 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835521142 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3T3 Cells Animals Apoptosis - drug effects Biocompatible Materials - pharmacology Cell Survival - drug effects Co-delivery Dendrimer Dendrimers - chemical synthesis Dendrimers - chemistry DNA - metabolism DOX Doxorubicin - pharmacology Drug Delivery Systems Drug Liberation Electrophoresis, Agar Gel Flow Cytometry Gene Transfer Techniques Graphene oxide Graphite - chemistry Humans Inhibitory Concentration 50 Matrix Metalloproteinase 9 - metabolism MCF-7 Cells Mice MMP-9 shRNA plasmid Plasmids - administration & dosage RNA, Small Interfering - administration & dosage Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman Static Electricity Thermogravimetry Transfection |
| title | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T11%3A54%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20polyamidoamne%20dendrimer%20functionalized%20graphene%20oxide%20for%20DOX%20and%20MMP-9%20shRNA%20plasmid%20co-delivery&rft.jtitle=Materials%20Science%20&%20Engineering%20C&rft.au=Gu,%20Yaming&rft.date=2017-01-01&rft.volume=70&rft.issue=Pt%201&rft.spage=572&rft.epage=585&rft.pages=572-585&rft.issn=0928-4931&rft.eissn=1873-0191&rft_id=info:doi/10.1016/j.msec.2016.09.035&rft_dat=%3Cproquest_cross%3E1835521142%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1835521142&rft_id=info:pmid/27770930&rft_els_id=S0928493116313686&rfr_iscdi=true |