Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells
Aim Physical activity is a potent way to impede vascular ageing. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation...
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| Veröffentlicht in: | Acta Physiologica 2017-06, Vol.220 (2), p.251-262 |
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| creator | Zietzer, A. Buschmann, E. E. Janke, D. Li, L. Brix, M. Meyborg, H. Stawowy, P. Jungk, C. Buschmann, I. Hillmeister, P. |
| description | Aim
Physical activity is a potent way to impede vascular ageing. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation and enhances endovascular fluid shear stress to increase collateral growth (arteriogenesis). To evaluate the effects of physical exercise and ISRT on the telomere biology of peripheral blood mononuclear cells (PBMCs), we conducted two clinical trials.
Methods
In the ISRT‐1 study, we assessed PBMC telomerase activity in 26 young healthy volunteers upon a single (short‐term) ISRT session and a single treadmill running session. In the ISRT‐2 study, we investigated PBMC telomere biology of 14 elderly patients with PAD, who underwent 30 h of (long‐term) ISRT within a 5‐week period.
Results
We demonstrate that telomerase activity significantly increased from 39.84 Total Product Generated (TPG) Units ± 6.15 to 58.10 TPG ± 10.46 upon a single treadmill running session in healthy volunteers. In the ISRT‐2 trial, PBMC telomerase activity and the mRNA expression of the telomere‐protective factor TRF2 increased from 40.87 TPG ± 4.45 to 60.98 TPG ± 6.83 and 2.10‐fold ± 0.40, respectively, upon long‐term ISRT in elderly patients with PAD.
Conclusion
In summary, we show that acute exercise and long‐term ISRT positively affect PBMC telomerase activity, which is indicative for an improved regenerative potential of immune cells and vascular tissues. Long‐term ISRT also enhances the gene expression of the telomere‐protective factor TRF2. |
| doi_str_mv | 10.1111/apha.12820 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835517739</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1899369786</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3570-7942c24a49b22564ea1411310ae0aeac0b5811d0f667082eece89e54661b63723</originalsourceid><addsrcrecordid>eNp9kc9q3DAQxkVp6YYklz5AEfRSAptoZFmyj0to_sBCckjORpZnawXZciW76d7yBCHPmCeJnE1z6KFiQGLm930M-gj5AuwY0jnRQ6uPgRecfSB7oESxBAXy4_ubFQtyGOMdYww4ZILzz2TBlVJMlMUeeVyZaUQ6tNtojXYU_2AwNiLVfUOd738-PzyNGDpq-8b-ts2UmNiiDjTopBtbDHrYpqkJqJNsROe71JsdzJgU4zyk7dTpng4Y7DArHK2d9w3tfO_7ybjZz6Bz8YB82mgX8fDt3ie3Zz9uTi-W66vzy9PVemmyXLGlKgU3XGhR1pznUqAGAZAB05hKG1bnBUDDNlKmD-CIBosScyEl1DJTPNsn33e-Q_C_Joxj1dk4b6B79FOsoMjyHJTKyoR--we981Po03aJKstMlqqQiTraUSb4GANuqiHYTodtBayag6rmoKrXoBL89c1yqjts3tG_sSQAdsC9dbj9j1W1ur5Y7UxfAEaRn_8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1899369786</pqid></control><display><type>article</type><title>Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Zietzer, A. ; Buschmann, E. E. ; Janke, D. ; Li, L. ; Brix, M. ; Meyborg, H. ; Stawowy, P. ; Jungk, C. ; Buschmann, I. ; Hillmeister, P.</creator><creatorcontrib>Zietzer, A. ; Buschmann, E. E. ; Janke, D. ; Li, L. ; Brix, M. ; Meyborg, H. ; Stawowy, P. ; Jungk, C. ; Buschmann, I. ; Hillmeister, P.</creatorcontrib><description>Aim
Physical activity is a potent way to impede vascular ageing. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation and enhances endovascular fluid shear stress to increase collateral growth (arteriogenesis). To evaluate the effects of physical exercise and ISRT on the telomere biology of peripheral blood mononuclear cells (PBMCs), we conducted two clinical trials.
Methods
In the ISRT‐1 study, we assessed PBMC telomerase activity in 26 young healthy volunteers upon a single (short‐term) ISRT session and a single treadmill running session. In the ISRT‐2 study, we investigated PBMC telomere biology of 14 elderly patients with PAD, who underwent 30 h of (long‐term) ISRT within a 5‐week period.
Results
We demonstrate that telomerase activity significantly increased from 39.84 Total Product Generated (TPG) Units ± 6.15 to 58.10 TPG ± 10.46 upon a single treadmill running session in healthy volunteers. In the ISRT‐2 trial, PBMC telomerase activity and the mRNA expression of the telomere‐protective factor TRF2 increased from 40.87 TPG ± 4.45 to 60.98 TPG ± 6.83 and 2.10‐fold ± 0.40, respectively, upon long‐term ISRT in elderly patients with PAD.
Conclusion
In summary, we show that acute exercise and long‐term ISRT positively affect PBMC telomerase activity, which is indicative for an improved regenerative potential of immune cells and vascular tissues. Long‐term ISRT also enhances the gene expression of the telomere‐protective factor TRF2.</description><identifier>ISSN: 1748-1708</identifier><identifier>EISSN: 1748-1716</identifier><identifier>DOI: 10.1111/apha.12820</identifier><identifier>PMID: 27770498</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Cardiovascular system ; Chromosomes ; Clinical trials ; counterpulsation ; Counterpulsation - methods ; Cross-Over Studies ; Exercise ; Exercise - physiology ; Female ; Fitness equipment ; Fluid flow ; Gene expression ; Geriatrics ; Humans ; individual shear rate therapy ; leucocyte ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - enzymology ; Male ; Mechanical stimuli ; Middle Aged ; Peripheral Arterial Disease - therapy ; peripheral artery disease ; Peripheral blood mononuclear cells ; Physical activity ; Physical fitness ; Physical training ; Running ; Shear stress ; Telomerase ; Telomerase - metabolism ; Telomere-binding protein ; TRF2 protein ; Young Adult</subject><ispartof>Acta Physiologica, 2017-06, Vol.220 (2), p.251-262</ispartof><rights>2016 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd</rights><rights>2016 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3570-7942c24a49b22564ea1411310ae0aeac0b5811d0f667082eece89e54661b63723</citedby><cites>FETCH-LOGICAL-c3570-7942c24a49b22564ea1411310ae0aeac0b5811d0f667082eece89e54661b63723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fapha.12820$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fapha.12820$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27770498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zietzer, A.</creatorcontrib><creatorcontrib>Buschmann, E. E.</creatorcontrib><creatorcontrib>Janke, D.</creatorcontrib><creatorcontrib>Li, L.</creatorcontrib><creatorcontrib>Brix, M.</creatorcontrib><creatorcontrib>Meyborg, H.</creatorcontrib><creatorcontrib>Stawowy, P.</creatorcontrib><creatorcontrib>Jungk, C.</creatorcontrib><creatorcontrib>Buschmann, I.</creatorcontrib><creatorcontrib>Hillmeister, P.</creatorcontrib><title>Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells</title><title>Acta Physiologica</title><addtitle>Acta Physiol (Oxf)</addtitle><description>Aim
Physical activity is a potent way to impede vascular ageing. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation and enhances endovascular fluid shear stress to increase collateral growth (arteriogenesis). To evaluate the effects of physical exercise and ISRT on the telomere biology of peripheral blood mononuclear cells (PBMCs), we conducted two clinical trials.
Methods
In the ISRT‐1 study, we assessed PBMC telomerase activity in 26 young healthy volunteers upon a single (short‐term) ISRT session and a single treadmill running session. In the ISRT‐2 study, we investigated PBMC telomere biology of 14 elderly patients with PAD, who underwent 30 h of (long‐term) ISRT within a 5‐week period.
Results
We demonstrate that telomerase activity significantly increased from 39.84 Total Product Generated (TPG) Units ± 6.15 to 58.10 TPG ± 10.46 upon a single treadmill running session in healthy volunteers. In the ISRT‐2 trial, PBMC telomerase activity and the mRNA expression of the telomere‐protective factor TRF2 increased from 40.87 TPG ± 4.45 to 60.98 TPG ± 6.83 and 2.10‐fold ± 0.40, respectively, upon long‐term ISRT in elderly patients with PAD.
Conclusion
In summary, we show that acute exercise and long‐term ISRT positively affect PBMC telomerase activity, which is indicative for an improved regenerative potential of immune cells and vascular tissues. Long‐term ISRT also enhances the gene expression of the telomere‐protective factor TRF2.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Cardiovascular system</subject><subject>Chromosomes</subject><subject>Clinical trials</subject><subject>counterpulsation</subject><subject>Counterpulsation - methods</subject><subject>Cross-Over Studies</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Female</subject><subject>Fitness equipment</subject><subject>Fluid flow</subject><subject>Gene expression</subject><subject>Geriatrics</subject><subject>Humans</subject><subject>individual shear rate therapy</subject><subject>leucocyte</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - enzymology</subject><subject>Male</subject><subject>Mechanical stimuli</subject><subject>Middle Aged</subject><subject>Peripheral Arterial Disease - therapy</subject><subject>peripheral artery disease</subject><subject>Peripheral blood mononuclear cells</subject><subject>Physical activity</subject><subject>Physical fitness</subject><subject>Physical training</subject><subject>Running</subject><subject>Shear stress</subject><subject>Telomerase</subject><subject>Telomerase - metabolism</subject><subject>Telomere-binding protein</subject><subject>TRF2 protein</subject><subject>Young Adult</subject><issn>1748-1708</issn><issn>1748-1716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9q3DAQxkVp6YYklz5AEfRSAptoZFmyj0to_sBCckjORpZnawXZciW76d7yBCHPmCeJnE1z6KFiQGLm930M-gj5AuwY0jnRQ6uPgRecfSB7oESxBAXy4_ubFQtyGOMdYww4ZILzz2TBlVJMlMUeeVyZaUQ6tNtojXYU_2AwNiLVfUOd738-PzyNGDpq-8b-ts2UmNiiDjTopBtbDHrYpqkJqJNsROe71JsdzJgU4zyk7dTpng4Y7DArHK2d9w3tfO_7ybjZz6Bz8YB82mgX8fDt3ie3Zz9uTi-W66vzy9PVemmyXLGlKgU3XGhR1pznUqAGAZAB05hKG1bnBUDDNlKmD-CIBosScyEl1DJTPNsn33e-Q_C_Joxj1dk4b6B79FOsoMjyHJTKyoR--we981Po03aJKstMlqqQiTraUSb4GANuqiHYTodtBayag6rmoKrXoBL89c1yqjts3tG_sSQAdsC9dbj9j1W1ur5Y7UxfAEaRn_8</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Zietzer, A.</creator><creator>Buschmann, E. E.</creator><creator>Janke, D.</creator><creator>Li, L.</creator><creator>Brix, M.</creator><creator>Meyborg, H.</creator><creator>Stawowy, P.</creator><creator>Jungk, C.</creator><creator>Buschmann, I.</creator><creator>Hillmeister, P.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TS</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells</title><author>Zietzer, A. ; Buschmann, E. E. ; Janke, D. ; Li, L. ; Brix, M. ; Meyborg, H. ; Stawowy, P. ; Jungk, C. ; Buschmann, I. ; Hillmeister, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3570-7942c24a49b22564ea1411310ae0aeac0b5811d0f667082eece89e54661b63723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Cardiovascular system</topic><topic>Chromosomes</topic><topic>Clinical trials</topic><topic>counterpulsation</topic><topic>Counterpulsation - methods</topic><topic>Cross-Over Studies</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Female</topic><topic>Fitness equipment</topic><topic>Fluid flow</topic><topic>Gene expression</topic><topic>Geriatrics</topic><topic>Humans</topic><topic>individual shear rate therapy</topic><topic>leucocyte</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - enzymology</topic><topic>Male</topic><topic>Mechanical stimuli</topic><topic>Middle Aged</topic><topic>Peripheral Arterial Disease - therapy</topic><topic>peripheral artery disease</topic><topic>Peripheral blood mononuclear cells</topic><topic>Physical activity</topic><topic>Physical fitness</topic><topic>Physical training</topic><topic>Running</topic><topic>Shear stress</topic><topic>Telomerase</topic><topic>Telomerase - metabolism</topic><topic>Telomere-binding protein</topic><topic>TRF2 protein</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zietzer, A.</creatorcontrib><creatorcontrib>Buschmann, E. E.</creatorcontrib><creatorcontrib>Janke, D.</creatorcontrib><creatorcontrib>Li, L.</creatorcontrib><creatorcontrib>Brix, M.</creatorcontrib><creatorcontrib>Meyborg, H.</creatorcontrib><creatorcontrib>Stawowy, P.</creatorcontrib><creatorcontrib>Jungk, C.</creatorcontrib><creatorcontrib>Buschmann, I.</creatorcontrib><creatorcontrib>Hillmeister, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Acta Physiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zietzer, A.</au><au>Buschmann, E. E.</au><au>Janke, D.</au><au>Li, L.</au><au>Brix, M.</au><au>Meyborg, H.</au><au>Stawowy, P.</au><au>Jungk, C.</au><au>Buschmann, I.</au><au>Hillmeister, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells</atitle><jtitle>Acta Physiologica</jtitle><addtitle>Acta Physiol (Oxf)</addtitle><date>2017-06</date><risdate>2017</risdate><volume>220</volume><issue>2</issue><spage>251</spage><epage>262</epage><pages>251-262</pages><issn>1748-1708</issn><eissn>1748-1716</eissn><abstract>Aim
Physical activity is a potent way to impede vascular ageing. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation and enhances endovascular fluid shear stress to increase collateral growth (arteriogenesis). To evaluate the effects of physical exercise and ISRT on the telomere biology of peripheral blood mononuclear cells (PBMCs), we conducted two clinical trials.
Methods
In the ISRT‐1 study, we assessed PBMC telomerase activity in 26 young healthy volunteers upon a single (short‐term) ISRT session and a single treadmill running session. In the ISRT‐2 study, we investigated PBMC telomere biology of 14 elderly patients with PAD, who underwent 30 h of (long‐term) ISRT within a 5‐week period.
Results
We demonstrate that telomerase activity significantly increased from 39.84 Total Product Generated (TPG) Units ± 6.15 to 58.10 TPG ± 10.46 upon a single treadmill running session in healthy volunteers. In the ISRT‐2 trial, PBMC telomerase activity and the mRNA expression of the telomere‐protective factor TRF2 increased from 40.87 TPG ± 4.45 to 60.98 TPG ± 6.83 and 2.10‐fold ± 0.40, respectively, upon long‐term ISRT in elderly patients with PAD.
Conclusion
In summary, we show that acute exercise and long‐term ISRT positively affect PBMC telomerase activity, which is indicative for an improved regenerative potential of immune cells and vascular tissues. Long‐term ISRT also enhances the gene expression of the telomere‐protective factor TRF2.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27770498</pmid><doi>10.1111/apha.12820</doi><tpages>12</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Cardiovascular system Chromosomes Clinical trials counterpulsation Counterpulsation - methods Cross-Over Studies Exercise Exercise - physiology Female Fitness equipment Fluid flow Gene expression Geriatrics Humans individual shear rate therapy leucocyte Leukocytes (mononuclear) Leukocytes, Mononuclear - enzymology Male Mechanical stimuli Middle Aged Peripheral Arterial Disease - therapy peripheral artery disease Peripheral blood mononuclear cells Physical activity Physical fitness Physical training Running Shear stress Telomerase Telomerase - metabolism Telomere-binding protein TRF2 protein Young Adult |
| title | Acute physical exercise and long‐term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells |
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