Effects of adenosine stimulation on the mRNA expression of CLCNKB in the basolateral medullary thick ascending limb of the rat kidney
Adenosine is a molecule produced by several organs within the body, including the kidneys, where it acts as an autoregulatory factor. It mediates ion transport in several nephron segments, including the proximal tubule and the thick ascending limb (TAL). Ion transport is dictated in part by anionic...
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| Veröffentlicht in: | Molecular medicine reports 2016-11, Vol.14 (5), p.4391-4398 |
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| description | Adenosine is a molecule produced by several organs within the body, including the kidneys, where it acts as an autoregulatory factor. It mediates ion transport in several nephron segments, including the proximal tubule and the thick ascending limb (TAL). Ion transport is dictated in part by anionic chloride channels, which regulate crucial kidney functions, and including the reabsorption of Na+ and Cl-, urine concentration, and establishing and maintaining the corticomedullary osmotic gradient. The present study investigated the effects of adenosine on the mRNA expression of chloride voltage-gated channel Kb (CLCNKB), a candidate gene involved in hypertension, which encodes for the ClC-Kb channel. Medullary thick ascending limb (mTAL) tubules were isolated from the rat kidney, and primary cultures of mTAL cells from the mTAL tubules were established. The cells were treated with adenosine and the mRNA expression of CLCNKB was detected by reverse transcription-quantitative polymerase chain reaction. The cells were also treated with pathways inhibitors (H8 and AACOCF3), and the protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP)-protein kinase A (PKA) and phospholipase A2 (PLA2) by were analyzed by western blotting. The findings indicated that adenosine increased the mRNA expression of CLCNKB in primary cultures of medullary TAL cells, and this stimulatory effect was regulated by the cAMP-PKA and PLA2-arachidonic acid (AA) pathways. The present study showed that adenosine affected the mRNA expression of CLCNKB, initially through the cAMP-PKA pathway and then the PLA2-AA pathway. |
| doi_str_mv | 10.3892/mmr.2016.5781 |
| format | Article |
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It mediates ion transport in several nephron segments, including the proximal tubule and the thick ascending limb (TAL). Ion transport is dictated in part by anionic chloride channels, which regulate crucial kidney functions, and including the reabsorption of Na+ and Cl-, urine concentration, and establishing and maintaining the corticomedullary osmotic gradient. The present study investigated the effects of adenosine on the mRNA expression of chloride voltage-gated channel Kb (CLCNKB), a candidate gene involved in hypertension, which encodes for the ClC-Kb channel. Medullary thick ascending limb (mTAL) tubules were isolated from the rat kidney, and primary cultures of mTAL cells from the mTAL tubules were established. The cells were treated with adenosine and the mRNA expression of CLCNKB was detected by reverse transcription-quantitative polymerase chain reaction. The cells were also treated with pathways inhibitors (H8 and AACOCF3), and the protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP)-protein kinase A (PKA) and phospholipase A2 (PLA2) by were analyzed by western blotting. The findings indicated that adenosine increased the mRNA expression of CLCNKB in primary cultures of medullary TAL cells, and this stimulatory effect was regulated by the cAMP-PKA and PLA2-arachidonic acid (AA) pathways. The present study showed that adenosine affected the mRNA expression of CLCNKB, initially through the cAMP-PKA pathway and then the PLA2-AA pathway.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2016.5781</identifier><identifier>PMID: 27748841</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>adenosine ; Adenosine - administration & dosage ; Adenosine - metabolism ; Animals ; Anion Transport Proteins - biosynthesis ; Anion Transport Proteins - genetics ; Arachidonic acid ; Arachidonic Acid - metabolism ; Arachidonic Acids - administration & dosage ; Chloride ; Chloride channels ; Chloride Channels - biosynthesis ; Chloride Channels - genetics ; chloride voltage-gated channel Kb ; Cyclic AMP ; Cyclic AMP - metabolism ; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors ; Cyclic AMP-Dependent Protein Kinases - biosynthesis ; Cyclic AMP-Dependent Protein Kinases - genetics ; Gene expression ; Gene Expression Regulation - drug effects ; Genetic aspects ; Health aspects ; Homeostasis ; Hypertension ; Ion channels ; Isoquinolines - administration & dosage ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - metabolism ; Kidneys ; Loop of Henle - drug effects ; Loop of Henle - metabolism ; Messenger RNA ; Nephrons - drug effects ; Nephrons - metabolism ; Phospholipase A2 ; Phospholipases A2 - biosynthesis ; Phospholipases A2 - genetics ; Polymerase chain reaction ; Potassium ; Primary Cell Culture ; Protein kinase A ; protein kinase A2 ; Rats ; Reabsorption ; Reverse transcription ; RNA, Messenger - biosynthesis ; Rodents ; Signal Transduction - drug effects ; Sodium ; Studies ; Tubules ; Urine ; Western blotting</subject><ispartof>Molecular medicine reports, 2016-11, Vol.14 (5), p.4391-4398</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c415t-44c0c54912538e8ac0055cb13ece2ace6076d43d8de93dae0612a6936fd1edf03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27748841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luan, Haiyan</creatorcontrib><creatorcontrib>Wu, Peng</creatorcontrib><creatorcontrib>Wang, Mingxiao</creatorcontrib><creatorcontrib>Sui, Hongyu</creatorcontrib><creatorcontrib>Fan, Lili</creatorcontrib><creatorcontrib>Gu, Ruimin</creatorcontrib><title>Effects of adenosine stimulation on the mRNA expression of CLCNKB in the basolateral medullary thick ascending limb of the rat kidney</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Adenosine is a molecule produced by several organs within the body, including the kidneys, where it acts as an autoregulatory factor. It mediates ion transport in several nephron segments, including the proximal tubule and the thick ascending limb (TAL). Ion transport is dictated in part by anionic chloride channels, which regulate crucial kidney functions, and including the reabsorption of Na+ and Cl-, urine concentration, and establishing and maintaining the corticomedullary osmotic gradient. The present study investigated the effects of adenosine on the mRNA expression of chloride voltage-gated channel Kb (CLCNKB), a candidate gene involved in hypertension, which encodes for the ClC-Kb channel. Medullary thick ascending limb (mTAL) tubules were isolated from the rat kidney, and primary cultures of mTAL cells from the mTAL tubules were established. The cells were treated with adenosine and the mRNA expression of CLCNKB was detected by reverse transcription-quantitative polymerase chain reaction. The cells were also treated with pathways inhibitors (H8 and AACOCF3), and the protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP)-protein kinase A (PKA) and phospholipase A2 (PLA2) by were analyzed by western blotting. The findings indicated that adenosine increased the mRNA expression of CLCNKB in primary cultures of medullary TAL cells, and this stimulatory effect was regulated by the cAMP-PKA and PLA2-arachidonic acid (AA) pathways. The present study showed that adenosine affected the mRNA expression of CLCNKB, initially through the cAMP-PKA pathway and then the PLA2-AA pathway.</description><subject>adenosine</subject><subject>Adenosine - administration & dosage</subject><subject>Adenosine - metabolism</subject><subject>Animals</subject><subject>Anion Transport Proteins - biosynthesis</subject><subject>Anion Transport Proteins - genetics</subject><subject>Arachidonic acid</subject><subject>Arachidonic Acid - metabolism</subject><subject>Arachidonic Acids - administration & dosage</subject><subject>Chloride</subject><subject>Chloride channels</subject><subject>Chloride Channels - biosynthesis</subject><subject>Chloride Channels - genetics</subject><subject>chloride voltage-gated channel Kb</subject><subject>Cyclic AMP</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors</subject><subject>Cyclic AMP-Dependent Protein Kinases - biosynthesis</subject><subject>Cyclic AMP-Dependent Protein Kinases - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Homeostasis</subject><subject>Hypertension</subject><subject>Ion channels</subject><subject>Isoquinolines - administration & dosage</subject><subject>Kidney Tubules, Proximal - drug effects</subject><subject>Kidney Tubules, Proximal - metabolism</subject><subject>Kidneys</subject><subject>Loop of Henle - drug effects</subject><subject>Loop of Henle - metabolism</subject><subject>Messenger RNA</subject><subject>Nephrons - drug effects</subject><subject>Nephrons - metabolism</subject><subject>Phospholipase A2</subject><subject>Phospholipases A2 - biosynthesis</subject><subject>Phospholipases A2 - genetics</subject><subject>Polymerase chain reaction</subject><subject>Potassium</subject><subject>Primary Cell Culture</subject><subject>Protein kinase A</subject><subject>protein kinase A2</subject><subject>Rats</subject><subject>Reabsorption</subject><subject>Reverse transcription</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Rodents</subject><subject>Signal Transduction - drug effects</subject><subject>Sodium</subject><subject>Studies</subject><subject>Tubules</subject><subject>Urine</subject><subject>Western blotting</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkV9rFDEUxQdRbK0--ioBH_Rl1vydSR7XpVZxqSD6HLLJTU07k6zJDNgP4Pc2010riiSQy83vXA73NM1zgldMKvpmHPOKYtKtRC_Jg-aU9Iq0DGP-8FhTpfqT5kkp1xh3ggr1uDmhfc-l5OS0-XnuPdipoOSRcRBTCRFQmcI4D2YKKaJ6p2-Axs-XawQ_9hlKuWt7tNluLj--ReEA7ExJVQLZDGgENw-Dybf1J9gbZIqF6EK8QkMYd4t2UWQzoZvgItw-bR55MxR4dnzPmq_vzr9s3rfbTxcfNuttazkRU8u5xVZwRahgEqSxGAthd4SBBWosdLjvHGdOOlDMGcAdoaZTrPOOgPOYnTWvD3P3OX2foUx6DNVatRohzUUTyQTnXJCuoi__Qa_TnGN1p4litFeUYvWHujID6BB9mrKxy1C95j1VUuF-oVb_oepxMAabIvhQ-38J2oPA5lRKBq_3OYx1n5pgvcSua-x6iV0vsVf-xdHsvKu7v6d_51yBVweg7E0NwqVyz9RJLeEtFi1nirBfH7a0iw</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Luan, Haiyan</creator><creator>Wu, Peng</creator><creator>Wang, Mingxiao</creator><creator>Sui, Hongyu</creator><creator>Fan, Lili</creator><creator>Gu, Ruimin</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20161101</creationdate><title>Effects of adenosine stimulation on the mRNA expression of CLCNKB in the basolateral medullary thick ascending limb of the rat kidney</title><author>Luan, Haiyan ; Wu, Peng ; Wang, Mingxiao ; Sui, Hongyu ; Fan, Lili ; Gu, Ruimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-44c0c54912538e8ac0055cb13ece2ace6076d43d8de93dae0612a6936fd1edf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>adenosine</topic><topic>Adenosine - administration & dosage</topic><topic>Adenosine - metabolism</topic><topic>Animals</topic><topic>Anion Transport Proteins - biosynthesis</topic><topic>Anion Transport Proteins - genetics</topic><topic>Arachidonic acid</topic><topic>Arachidonic Acid - metabolism</topic><topic>Arachidonic Acids - administration & dosage</topic><topic>Chloride</topic><topic>Chloride channels</topic><topic>Chloride Channels - biosynthesis</topic><topic>Chloride Channels - genetics</topic><topic>chloride voltage-gated channel Kb</topic><topic>Cyclic AMP</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors</topic><topic>Cyclic AMP-Dependent Protein Kinases - biosynthesis</topic><topic>Cyclic AMP-Dependent Protein Kinases - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Homeostasis</topic><topic>Hypertension</topic><topic>Ion channels</topic><topic>Isoquinolines - administration & dosage</topic><topic>Kidney Tubules, Proximal - drug effects</topic><topic>Kidney Tubules, Proximal - metabolism</topic><topic>Kidneys</topic><topic>Loop of Henle - drug effects</topic><topic>Loop of Henle - metabolism</topic><topic>Messenger RNA</topic><topic>Nephrons - drug effects</topic><topic>Nephrons - metabolism</topic><topic>Phospholipase A2</topic><topic>Phospholipases A2 - biosynthesis</topic><topic>Phospholipases A2 - genetics</topic><topic>Polymerase chain reaction</topic><topic>Potassium</topic><topic>Primary Cell Culture</topic><topic>Protein kinase A</topic><topic>protein kinase A2</topic><topic>Rats</topic><topic>Reabsorption</topic><topic>Reverse transcription</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Rodents</topic><topic>Signal Transduction - drug effects</topic><topic>Sodium</topic><topic>Studies</topic><topic>Tubules</topic><topic>Urine</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luan, Haiyan</creatorcontrib><creatorcontrib>Wu, Peng</creatorcontrib><creatorcontrib>Wang, Mingxiao</creatorcontrib><creatorcontrib>Sui, Hongyu</creatorcontrib><creatorcontrib>Fan, Lili</creatorcontrib><creatorcontrib>Gu, Ruimin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luan, Haiyan</au><au>Wu, Peng</au><au>Wang, Mingxiao</au><au>Sui, Hongyu</au><au>Fan, Lili</au><au>Gu, Ruimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of adenosine stimulation on the mRNA expression of CLCNKB in the basolateral medullary thick ascending limb of the rat kidney</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>14</volume><issue>5</issue><spage>4391</spage><epage>4398</epage><pages>4391-4398</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Adenosine is a molecule produced by several organs within the body, including the kidneys, where it acts as an autoregulatory factor. It mediates ion transport in several nephron segments, including the proximal tubule and the thick ascending limb (TAL). Ion transport is dictated in part by anionic chloride channels, which regulate crucial kidney functions, and including the reabsorption of Na+ and Cl-, urine concentration, and establishing and maintaining the corticomedullary osmotic gradient. The present study investigated the effects of adenosine on the mRNA expression of chloride voltage-gated channel Kb (CLCNKB), a candidate gene involved in hypertension, which encodes for the ClC-Kb channel. Medullary thick ascending limb (mTAL) tubules were isolated from the rat kidney, and primary cultures of mTAL cells from the mTAL tubules were established. The cells were treated with adenosine and the mRNA expression of CLCNKB was detected by reverse transcription-quantitative polymerase chain reaction. The cells were also treated with pathways inhibitors (H8 and AACOCF3), and the protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP)-protein kinase A (PKA) and phospholipase A2 (PLA2) by were analyzed by western blotting. The findings indicated that adenosine increased the mRNA expression of CLCNKB in primary cultures of medullary TAL cells, and this stimulatory effect was regulated by the cAMP-PKA and PLA2-arachidonic acid (AA) pathways. The present study showed that adenosine affected the mRNA expression of CLCNKB, initially through the cAMP-PKA pathway and then the PLA2-AA pathway.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27748841</pmid><doi>10.3892/mmr.2016.5781</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | adenosine Adenosine - administration & dosage Adenosine - metabolism Animals Anion Transport Proteins - biosynthesis Anion Transport Proteins - genetics Arachidonic acid Arachidonic Acid - metabolism Arachidonic Acids - administration & dosage Chloride Chloride channels Chloride Channels - biosynthesis Chloride Channels - genetics chloride voltage-gated channel Kb Cyclic AMP Cyclic AMP - metabolism Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors Cyclic AMP-Dependent Protein Kinases - biosynthesis Cyclic AMP-Dependent Protein Kinases - genetics Gene expression Gene Expression Regulation - drug effects Genetic aspects Health aspects Homeostasis Hypertension Ion channels Isoquinolines - administration & dosage Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - metabolism Kidneys Loop of Henle - drug effects Loop of Henle - metabolism Messenger RNA Nephrons - drug effects Nephrons - metabolism Phospholipase A2 Phospholipases A2 - biosynthesis Phospholipases A2 - genetics Polymerase chain reaction Potassium Primary Cell Culture Protein kinase A protein kinase A2 Rats Reabsorption Reverse transcription RNA, Messenger - biosynthesis Rodents Signal Transduction - drug effects Sodium Studies Tubules Urine Western blotting |
| title | Effects of adenosine stimulation on the mRNA expression of CLCNKB in the basolateral medullary thick ascending limb of the rat kidney |
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