Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus
Antibiotic A201A produced by Saccharothrix mutabilis subsp. capreolus NRRL3817 contains an aminonucleoside ( N 6 , N 6 -dimethyl-3′-amino-3′-deoxyadenosyl), a polyketide ( α -methyl- p -coumaric acid) and a disaccharide moiety. The heterologous expression in Streptomyces lividans and Streptomyces co...
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| Veröffentlicht in: | Journal of antibiotics 2017-04, Vol.70 (4), p.404-413 |
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| creator | Saugar, Irene Molloy, Brian Sanz, Eloisa Blanca Sánchez, María Fernández-Lobato, María Jiménez, Antonio |
| description | Antibiotic A201A produced by
Saccharothrix mutabilis
subsp.
capreolus
NRRL3817 contains an aminonucleoside (
N
6
,
N
6
-dimethyl-3′-amino-3′-deoxyadenosyl), a polyketide (
α
-methyl-
p
-coumaric acid) and a disaccharide moiety. The heterologous expression in
Streptomyces lividans
and
Streptomyces coelicolor
of a
S. mutabilis
genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (
ata
). The analysis of the nucleotide sequence of the fragment reveals the presence of 32 putative open reading frames (ORF), 28 of which according to boundary gene inactivation experiments are likely to be sufficient for A201A biosynthesis. Most of these ORFs could be assigned to the biosynthesis of the antibiotic three structural moieties. Indeed, five ORFs had been previously implicated in the biosynthesis of the aminonucleoside moiety, at least nine were related to the biosynthesis of the polyketide (
ata-PKS1
-
ataPKS4, ata18, ata19, ata2, ata4
and
ata7
) and six were associated with the synthesis of the disaccharide (
ata12
,
ata13, ata16, ata17, ata5
and
ata10
) moieties. In addition to AtaP5, three putative methyltransferase genes are also found in the
ata
cluster (Ata6, Ata8 and Ata11), and no regulatory genes were found. |
| doi_str_mv | 10.1038/ja.2016.123 |
| format | Article |
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Saccharothrix mutabilis
subsp.
capreolus
NRRL3817 contains an aminonucleoside (
N
6
,
N
6
-dimethyl-3′-amino-3′-deoxyadenosyl), a polyketide (
α
-methyl-
p
-coumaric acid) and a disaccharide moiety. The heterologous expression in
Streptomyces lividans
and
Streptomyces coelicolor
of a
S. mutabilis
genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (
ata
). The analysis of the nucleotide sequence of the fragment reveals the presence of 32 putative open reading frames (ORF), 28 of which according to boundary gene inactivation experiments are likely to be sufficient for A201A biosynthesis. Most of these ORFs could be assigned to the biosynthesis of the antibiotic three structural moieties. Indeed, five ORFs had been previously implicated in the biosynthesis of the aminonucleoside moiety, at least nine were related to the biosynthesis of the polyketide (
ata-PKS1
-
ataPKS4, ata18, ata19, ata2, ata4
and
ata7
) and six were associated with the synthesis of the disaccharide (
ata12
,
ata13, ata16, ata17, ata5
and
ata10
) moieties. In addition to AtaP5, three putative methyltransferase genes are also found in the
ata
cluster (Ata6, Ata8 and Ata11), and no regulatory genes were found.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/ja.2016.123</identifier><identifier>PMID: 27731336</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/22 ; 45/23 ; 45/41 ; 631/45 ; 631/61 ; 82/16 ; Actinomycetales - genetics ; Amino Acid Sequence ; Aminoglycosides - biosynthesis ; Aminoglycosides - genetics ; Anti-Bacterial Agents - biosynthesis ; Antibiotics ; Bacteriology ; Base Sequence ; Biomedical and Life Sciences ; Bioorganic Chemistry ; Biosynthesis ; Computational Biology ; Coumaric acid ; Disaccharides ; Disaccharides - biosynthesis ; Disaccharides - genetics ; Gene Targeting ; Genes ; Inactivation ; Life Sciences ; Medicinal Chemistry ; Methyltransferase ; Methyltransferases - genetics ; Microbiology ; Multigene Family - genetics ; Nucleotide sequence ; Nucleotides ; Oligonucleotides - chemistry ; Open reading frames ; Organic Chemistry ; original-article ; p-Coumaric acid ; Plasmids ; Polyketides - metabolism ; Streptomyces - genetics ; Streptomyces - metabolism</subject><ispartof>Journal of antibiotics, 2017-04, Vol.70 (4), p.404-413</ispartof><rights>Japan Antibiotics Research Association 2017</rights><rights>Copyright Nature Publishing Group Apr 2017</rights><rights>Japan Antibiotics Research Association 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3583-7edda6503b68908d163862335534c362d37063a822c5f33acd2bd06dcae994763</citedby><cites>FETCH-LOGICAL-c3583-7edda6503b68908d163862335534c362d37063a822c5f33acd2bd06dcae994763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27731336$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saugar, Irene</creatorcontrib><creatorcontrib>Molloy, Brian</creatorcontrib><creatorcontrib>Sanz, Eloisa</creatorcontrib><creatorcontrib>Blanca Sánchez, María</creatorcontrib><creatorcontrib>Fernández-Lobato, María</creatorcontrib><creatorcontrib>Jiménez, Antonio</creatorcontrib><title>Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>Antibiotic A201A produced by
Saccharothrix mutabilis
subsp.
capreolus
NRRL3817 contains an aminonucleoside (
N
6
,
N
6
-dimethyl-3′-amino-3′-deoxyadenosyl), a polyketide (
α
-methyl-
p
-coumaric acid) and a disaccharide moiety. The heterologous expression in
Streptomyces lividans
and
Streptomyces coelicolor
of a
S. mutabilis
genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (
ata
). The analysis of the nucleotide sequence of the fragment reveals the presence of 32 putative open reading frames (ORF), 28 of which according to boundary gene inactivation experiments are likely to be sufficient for A201A biosynthesis. Most of these ORFs could be assigned to the biosynthesis of the antibiotic three structural moieties. Indeed, five ORFs had been previously implicated in the biosynthesis of the aminonucleoside moiety, at least nine were related to the biosynthesis of the polyketide (
ata-PKS1
-
ataPKS4, ata18, ata19, ata2, ata4
and
ata7
) and six were associated with the synthesis of the disaccharide (
ata12
,
ata13, ata16, ata17, ata5
and
ata10
) moieties. In addition to AtaP5, three putative methyltransferase genes are also found in the
ata
cluster (Ata6, Ata8 and Ata11), and no regulatory genes were found.</description><subject>38/22</subject><subject>45/23</subject><subject>45/41</subject><subject>631/45</subject><subject>631/61</subject><subject>82/16</subject><subject>Actinomycetales - genetics</subject><subject>Amino Acid Sequence</subject><subject>Aminoglycosides - biosynthesis</subject><subject>Aminoglycosides - genetics</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Antibiotics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Bioorganic Chemistry</subject><subject>Biosynthesis</subject><subject>Computational Biology</subject><subject>Coumaric acid</subject><subject>Disaccharides</subject><subject>Disaccharides - biosynthesis</subject><subject>Disaccharides - genetics</subject><subject>Gene Targeting</subject><subject>Genes</subject><subject>Inactivation</subject><subject>Life Sciences</subject><subject>Medicinal Chemistry</subject><subject>Methyltransferase</subject><subject>Methyltransferases - genetics</subject><subject>Microbiology</subject><subject>Multigene Family - genetics</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Oligonucleotides - chemistry</subject><subject>Open reading frames</subject><subject>Organic Chemistry</subject><subject>original-article</subject><subject>p-Coumaric acid</subject><subject>Plasmids</subject><subject>Polyketides - metabolism</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUGL1DAYhoMo7rh68i4BLyvaMcnXpulxGNxVWPCgnsPXJN3J0CZj0sKuP8LfbMZZRUS8JIE83_t88BLynLM1Z6De7nEtGJdrLuABWXGleMVr2T0kK8YEr5QS7Iw8yXnPGLTQqsfkTLQtcAC5It-3O0xoZpf8N5x9DDQOdN452vuY70J5zd7QGxccNeOSC0cvcMZXdIjpJ7cp7g3FyYcYFjO6mL11FMPsS8Jxdkhxop_QmCKK8y75WzotM_Z-9Jnmpc-HNTV4SC6W_Kfk0YBjds_u73Py5fLd5-376vrj1Yft5roy0CioWmctyoZBL1XHlOUSlBQATQO1ASkstEwCKiFMMwCgsaK3TFqDruvqVsI5uTjlHlL8urg868ln48YRg4tL1lxBU5dDqoK-_AvdxyWFsp0Wkhej6KD5H1UaqVnHRXPUvj5RJsWckxv0IfkJ053mTB_L1HvUxzJ1KbPQL-4zl35y9jf7q70CvDkBuXyFG5f-kP4j7wfRYafO</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Saugar, Irene</creator><creator>Molloy, Brian</creator><creator>Sanz, Eloisa</creator><creator>Blanca Sánchez, María</creator><creator>Fernández-Lobato, María</creator><creator>Jiménez, Antonio</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20170401</creationdate><title>Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus</title><author>Saugar, Irene ; Molloy, Brian ; Sanz, Eloisa ; Blanca Sánchez, María ; Fernández-Lobato, María ; Jiménez, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3583-7edda6503b68908d163862335534c362d37063a822c5f33acd2bd06dcae994763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>38/22</topic><topic>45/23</topic><topic>45/41</topic><topic>631/45</topic><topic>631/61</topic><topic>82/16</topic><topic>Actinomycetales - genetics</topic><topic>Amino Acid Sequence</topic><topic>Aminoglycosides - biosynthesis</topic><topic>Aminoglycosides - genetics</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Antibiotics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biomedical and Life Sciences</topic><topic>Bioorganic Chemistry</topic><topic>Biosynthesis</topic><topic>Computational Biology</topic><topic>Coumaric acid</topic><topic>Disaccharides</topic><topic>Disaccharides - biosynthesis</topic><topic>Disaccharides - genetics</topic><topic>Gene Targeting</topic><topic>Genes</topic><topic>Inactivation</topic><topic>Life Sciences</topic><topic>Medicinal Chemistry</topic><topic>Methyltransferase</topic><topic>Methyltransferases - genetics</topic><topic>Microbiology</topic><topic>Multigene Family - genetics</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Oligonucleotides - chemistry</topic><topic>Open reading frames</topic><topic>Organic Chemistry</topic><topic>original-article</topic><topic>p-Coumaric acid</topic><topic>Plasmids</topic><topic>Polyketides - metabolism</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saugar, Irene</creatorcontrib><creatorcontrib>Molloy, Brian</creatorcontrib><creatorcontrib>Sanz, Eloisa</creatorcontrib><creatorcontrib>Blanca Sánchez, María</creatorcontrib><creatorcontrib>Fernández-Lobato, María</creatorcontrib><creatorcontrib>Jiménez, Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antibiotics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saugar, Irene</au><au>Molloy, Brian</au><au>Sanz, Eloisa</au><au>Blanca Sánchez, María</au><au>Fernández-Lobato, María</au><au>Jiménez, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus</atitle><jtitle>Journal of antibiotics</jtitle><stitle>J Antibiot</stitle><addtitle>J Antibiot (Tokyo)</addtitle><date>2017-04-01</date><risdate>2017</risdate><volume>70</volume><issue>4</issue><spage>404</spage><epage>413</epage><pages>404-413</pages><issn>0021-8820</issn><eissn>1881-1469</eissn><abstract>Antibiotic A201A produced by
Saccharothrix mutabilis
subsp.
capreolus
NRRL3817 contains an aminonucleoside (
N
6
,
N
6
-dimethyl-3′-amino-3′-deoxyadenosyl), a polyketide (
α
-methyl-
p
-coumaric acid) and a disaccharide moiety. The heterologous expression in
Streptomyces lividans
and
Streptomyces coelicolor
of a
S. mutabilis
genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (
ata
). The analysis of the nucleotide sequence of the fragment reveals the presence of 32 putative open reading frames (ORF), 28 of which according to boundary gene inactivation experiments are likely to be sufficient for A201A biosynthesis. Most of these ORFs could be assigned to the biosynthesis of the antibiotic three structural moieties. Indeed, five ORFs had been previously implicated in the biosynthesis of the aminonucleoside moiety, at least nine were related to the biosynthesis of the polyketide (
ata-PKS1
-
ataPKS4, ata18, ata19, ata2, ata4
and
ata7
) and six were associated with the synthesis of the disaccharide (
ata12
,
ata13, ata16, ata17, ata5
and
ata10
) moieties. In addition to AtaP5, three putative methyltransferase genes are also found in the
ata
cluster (Ata6, Ata8 and Ata11), and no regulatory genes were found.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27731336</pmid><doi>10.1038/ja.2016.123</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8820 |
| ispartof | Journal of antibiotics, 2017-04, Vol.70 (4), p.404-413 |
| issn | 0021-8820 1881-1469 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835418368 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 38/22 45/23 45/41 631/45 631/61 82/16 Actinomycetales - genetics Amino Acid Sequence Aminoglycosides - biosynthesis Aminoglycosides - genetics Anti-Bacterial Agents - biosynthesis Antibiotics Bacteriology Base Sequence Biomedical and Life Sciences Bioorganic Chemistry Biosynthesis Computational Biology Coumaric acid Disaccharides Disaccharides - biosynthesis Disaccharides - genetics Gene Targeting Genes Inactivation Life Sciences Medicinal Chemistry Methyltransferase Methyltransferases - genetics Microbiology Multigene Family - genetics Nucleotide sequence Nucleotides Oligonucleotides - chemistry Open reading frames Organic Chemistry original-article p-Coumaric acid Plasmids Polyketides - metabolism Streptomyces - genetics Streptomyces - metabolism |
| title | Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus |
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