Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos
Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study,...
Gespeichert in:
| Veröffentlicht in: | Theriogenology 2017-01, Vol.87, p.298-305 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 305 |
|---|---|
| container_issue | |
| container_start_page | 298 |
| container_title | Theriogenology |
| container_volume | 87 |
| creator | Jin, Long Zhu, Hai-Ying Guo, Qing Li, Xiao-Chen Zhang, Yu-Chen Cui, Cheng-Du Li, Wen-Xue Cui, Zheng-Yun Yin, Xi-Jun Kang, Jin-Dan |
| description | Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12. We compared the in vitro embryonic development of SCNT embryos treated with different concentrations of MGCD0103 for 24 hours. Our results reported that treating with 0.2-μM MGCD0103 for 24 hours effectively improved the development of SCNT embryos, in comparison to the control group (blastocyst formation rate, 25.5 vs. 10.7%, P |
| doi_str_mv | 10.1016/j.theriogenology.2016.09.011 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1835414009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X16304241</els_id><sourcerecordid>1835414009</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-a11440ad59c8b1a2df48eec9ae86ed0b37efe562316d36180f90455efee7615e3</originalsourceid><addsrcrecordid>eNqNUU2P0zAUtBCILYW_gHzgwGET7DhxEokLKvslLeICEjfLsV9aV7EdbHdRfyD_C0ctlbhxsvw0M-_NDELvKCkpofzDvkw7CMZvwfnJb49llacl6UtC6TO0ol3bF6xi9DlaEdKzgvf0xxV6FeOeEMI4py_RVdW2dVUTtkK_b8YRVMJ-xDsTk3eApYJ0nGQy3mHrtRmNOn1-mbTDX-42nwkl7BrLC0PDmRMBG7czg0k-XONMcQc1gQw4wBz8Nkhrjdti6TTOJjLvCSY_W3BJTlh5O0MCp2C5ZvZBmawdvc3bFVYwTRe5FKSLIwQMdghHH1-jF6OcIrw5v2v0_fbm2-a-ePx697D59Fgo1vFUSErrmkjd9KobqKz0WHcAqpfQcdBkYC2M0PAcHteM046MPambJg-h5bQBtkbvT7rZzc8DxCSsictl0oE_REE71tS0XnJfo48nqAo-xgCjmIOxMhwFJWIpUuzFv0WKpUhBepGLzPS3502HwYK-kP82lwG3JwBkv08GgojKLOFpE3KhQnvzf5v-ALUSvcs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835414009</pqid></control><display><type>article</type><title>Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Jin, Long ; Zhu, Hai-Ying ; Guo, Qing ; Li, Xiao-Chen ; Zhang, Yu-Chen ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</creator><creatorcontrib>Jin, Long ; Zhu, Hai-Ying ; Guo, Qing ; Li, Xiao-Chen ; Zhang, Yu-Chen ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</creatorcontrib><description>Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12. We compared the in vitro embryonic development of SCNT embryos treated with different concentrations of MGCD0103 for 24 hours. Our results reported that treating with 0.2-μM MGCD0103 for 24 hours effectively improved the development of SCNT embryos, in comparison to the control group (blastocyst formation rate, 25.5 vs. 10.7%, P < 0.05). Then we tested the in vitro development of SCNT embryos treated with 0.2-μM MGCD0103 for various intervals after activation. Treatment for 6 hours significantly improved the development of pig SCNT embryos, compared with the control group (blastocyst formation rate, 21.2 vs. 10.5%, P < 0.05). Furthermore, MGCD0103 supplementation significantly (P < 0.05) increases the average fluorescence intensity of AcH3K9 and AcH4K12 in embryos at the pseudo-pronuclear stage. To examine the in vivo development, MGCD0103-treated SCNT embryos were transferred into two surrogate sows, one of whom became pregnant and three fetuses developed. These results suggest that MGCD0103 can enhance the nuclear reprogramming and improve in vitro developmental potential of porcine SCNT embryos.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2016.09.011</identifier><identifier>PMID: 27742403</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylation - drug effects ; Animals ; Benzamides - pharmacology ; Cellular Reprogramming - drug effects ; Cloning, Organism ; Embryo, Mammalian - drug effects ; Female ; Histone deacetylase inhibitors (HDACi) ; Histone Deacetylase Inhibitors - pharmacology ; Histones - metabolism ; In vitro development ; MGCD0103 ; Nuclear Transfer Techniques - veterinary ; Porcine ; Pregnancy ; Pyrimidines - pharmacology ; Somatic cell nuclear transfer (SCNT) ; Swine - embryology</subject><ispartof>Theriogenology, 2017-01, Vol.87, p.298-305</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-a11440ad59c8b1a2df48eec9ae86ed0b37efe562316d36180f90455efee7615e3</citedby><cites>FETCH-LOGICAL-c386t-a11440ad59c8b1a2df48eec9ae86ed0b37efe562316d36180f90455efee7615e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2016.09.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27742403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhu, Hai-Ying</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><creatorcontrib>Li, Xiao-Chen</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Cui, Cheng-Du</creatorcontrib><creatorcontrib>Li, Wen-Xue</creatorcontrib><creatorcontrib>Cui, Zheng-Yun</creatorcontrib><creatorcontrib>Yin, Xi-Jun</creatorcontrib><creatorcontrib>Kang, Jin-Dan</creatorcontrib><title>Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12. We compared the in vitro embryonic development of SCNT embryos treated with different concentrations of MGCD0103 for 24 hours. Our results reported that treating with 0.2-μM MGCD0103 for 24 hours effectively improved the development of SCNT embryos, in comparison to the control group (blastocyst formation rate, 25.5 vs. 10.7%, P < 0.05). Then we tested the in vitro development of SCNT embryos treated with 0.2-μM MGCD0103 for various intervals after activation. Treatment for 6 hours significantly improved the development of pig SCNT embryos, compared with the control group (blastocyst formation rate, 21.2 vs. 10.5%, P < 0.05). Furthermore, MGCD0103 supplementation significantly (P < 0.05) increases the average fluorescence intensity of AcH3K9 and AcH4K12 in embryos at the pseudo-pronuclear stage. To examine the in vivo development, MGCD0103-treated SCNT embryos were transferred into two surrogate sows, one of whom became pregnant and three fetuses developed. These results suggest that MGCD0103 can enhance the nuclear reprogramming and improve in vitro developmental potential of porcine SCNT embryos.</description><subject>Acetylation - drug effects</subject><subject>Animals</subject><subject>Benzamides - pharmacology</subject><subject>Cellular Reprogramming - drug effects</subject><subject>Cloning, Organism</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Female</subject><subject>Histone deacetylase inhibitors (HDACi)</subject><subject>Histone Deacetylase Inhibitors - pharmacology</subject><subject>Histones - metabolism</subject><subject>In vitro development</subject><subject>MGCD0103</subject><subject>Nuclear Transfer Techniques - veterinary</subject><subject>Porcine</subject><subject>Pregnancy</subject><subject>Pyrimidines - pharmacology</subject><subject>Somatic cell nuclear transfer (SCNT)</subject><subject>Swine - embryology</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU2P0zAUtBCILYW_gHzgwGET7DhxEokLKvslLeICEjfLsV9aV7EdbHdRfyD_C0ctlbhxsvw0M-_NDELvKCkpofzDvkw7CMZvwfnJb49llacl6UtC6TO0ol3bF6xi9DlaEdKzgvf0xxV6FeOeEMI4py_RVdW2dVUTtkK_b8YRVMJ-xDsTk3eApYJ0nGQy3mHrtRmNOn1-mbTDX-42nwkl7BrLC0PDmRMBG7czg0k-XONMcQc1gQw4wBz8Nkhrjdti6TTOJjLvCSY_W3BJTlh5O0MCp2C5ZvZBmawdvc3bFVYwTRe5FKSLIwQMdghHH1-jF6OcIrw5v2v0_fbm2-a-ePx697D59Fgo1vFUSErrmkjd9KobqKz0WHcAqpfQcdBkYC2M0PAcHteM046MPambJg-h5bQBtkbvT7rZzc8DxCSsictl0oE_REE71tS0XnJfo48nqAo-xgCjmIOxMhwFJWIpUuzFv0WKpUhBepGLzPS3502HwYK-kP82lwG3JwBkv08GgojKLOFpE3KhQnvzf5v-ALUSvcs</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Jin, Long</creator><creator>Zhu, Hai-Ying</creator><creator>Guo, Qing</creator><creator>Li, Xiao-Chen</creator><creator>Zhang, Yu-Chen</creator><creator>Cui, Cheng-Du</creator><creator>Li, Wen-Xue</creator><creator>Cui, Zheng-Yun</creator><creator>Yin, Xi-Jun</creator><creator>Kang, Jin-Dan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos</title><author>Jin, Long ; Zhu, Hai-Ying ; Guo, Qing ; Li, Xiao-Chen ; Zhang, Yu-Chen ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-a11440ad59c8b1a2df48eec9ae86ed0b37efe562316d36180f90455efee7615e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acetylation - drug effects</topic><topic>Animals</topic><topic>Benzamides - pharmacology</topic><topic>Cellular Reprogramming - drug effects</topic><topic>Cloning, Organism</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Female</topic><topic>Histone deacetylase inhibitors (HDACi)</topic><topic>Histone Deacetylase Inhibitors - pharmacology</topic><topic>Histones - metabolism</topic><topic>In vitro development</topic><topic>MGCD0103</topic><topic>Nuclear Transfer Techniques - veterinary</topic><topic>Porcine</topic><topic>Pregnancy</topic><topic>Pyrimidines - pharmacology</topic><topic>Somatic cell nuclear transfer (SCNT)</topic><topic>Swine - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhu, Hai-Ying</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><creatorcontrib>Li, Xiao-Chen</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Cui, Cheng-Du</creatorcontrib><creatorcontrib>Li, Wen-Xue</creatorcontrib><creatorcontrib>Cui, Zheng-Yun</creatorcontrib><creatorcontrib>Yin, Xi-Jun</creatorcontrib><creatorcontrib>Kang, Jin-Dan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Long</au><au>Zhu, Hai-Ying</au><au>Guo, Qing</au><au>Li, Xiao-Chen</au><au>Zhang, Yu-Chen</au><au>Cui, Cheng-Du</au><au>Li, Wen-Xue</au><au>Cui, Zheng-Yun</au><au>Yin, Xi-Jun</au><au>Kang, Jin-Dan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>87</volume><spage>298</spage><epage>305</epage><pages>298-305</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12. We compared the in vitro embryonic development of SCNT embryos treated with different concentrations of MGCD0103 for 24 hours. Our results reported that treating with 0.2-μM MGCD0103 for 24 hours effectively improved the development of SCNT embryos, in comparison to the control group (blastocyst formation rate, 25.5 vs. 10.7%, P < 0.05). Then we tested the in vitro development of SCNT embryos treated with 0.2-μM MGCD0103 for various intervals after activation. Treatment for 6 hours significantly improved the development of pig SCNT embryos, compared with the control group (blastocyst formation rate, 21.2 vs. 10.5%, P < 0.05). Furthermore, MGCD0103 supplementation significantly (P < 0.05) increases the average fluorescence intensity of AcH3K9 and AcH4K12 in embryos at the pseudo-pronuclear stage. To examine the in vivo development, MGCD0103-treated SCNT embryos were transferred into two surrogate sows, one of whom became pregnant and three fetuses developed. These results suggest that MGCD0103 can enhance the nuclear reprogramming and improve in vitro developmental potential of porcine SCNT embryos.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27742403</pmid><doi>10.1016/j.theriogenology.2016.09.011</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-691X |
| ispartof | Theriogenology, 2017-01, Vol.87, p.298-305 |
| issn | 0093-691X 1879-3231 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835414009 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acetylation - drug effects Animals Benzamides - pharmacology Cellular Reprogramming - drug effects Cloning, Organism Embryo, Mammalian - drug effects Female Histone deacetylase inhibitors (HDACi) Histone Deacetylase Inhibitors - pharmacology Histones - metabolism In vitro development MGCD0103 Nuclear Transfer Techniques - veterinary Porcine Pregnancy Pyrimidines - pharmacology Somatic cell nuclear transfer (SCNT) Swine - embryology |
| title | Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T08%3A22%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20histone%20acetylation%20modification%20with%20MGCD0103,%20a%20histone%20deacetylase%20inhibitor,%20on%20nuclear%20reprogramming%20and%20the%20developmental%20competence%20of%20porcine%20somatic%20cell%20nuclear%20transfer%20embryos&rft.jtitle=Theriogenology&rft.au=Jin,%20Long&rft.date=2017-01-01&rft.volume=87&rft.spage=298&rft.epage=305&rft.pages=298-305&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/j.theriogenology.2016.09.011&rft_dat=%3Cproquest_cross%3E1835414009%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1835414009&rft_id=info:pmid/27742403&rft_els_id=S0093691X16304241&rfr_iscdi=true |