Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro
[Display omitted] •Momordica charantia prevent embryogenesis in Fasciola hepatica eggs.•Anti-ovicidal activity against F. hepatica of the flavonoid quercetin M. charantia.•Momordica charantia is a potential alternative to control helminth parasites. Fasciolosis, a parasitic disease caused by the tre...
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creator | Pereira, Cíntia A.J. Oliveira, Laura L.S. Coaglio, Aytube L. Santos, Fernanda S.O. Cezar, Rodolfo S.M. Mendes, Tiago Oliveira, Fernando L.P. Conzensa, Gustavo Lima, Walter S. |
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•Momordica charantia prevent embryogenesis in Fasciola hepatica eggs.•Anti-ovicidal activity against F. hepatica of the flavonoid quercetin M. charantia.•Momordica charantia is a potential alternative to control helminth parasites.
Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid–liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC–MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions. |
doi_str_mv | 10.1016/j.vetpar.2016.08.025 |
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•Momordica charantia prevent embryogenesis in Fasciola hepatica eggs.•Anti-ovicidal activity against F. hepatica of the flavonoid quercetin M. charantia.•Momordica charantia is a potential alternative to control helminth parasites.
Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid–liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC–MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2016.08.025</identifier><identifier>PMID: 27692319</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Albendazole - analogs & derivatives ; Albendazole - pharmacology ; Animals ; Anthelmintics - pharmacology ; Anti-ovicidal activity ; Cattle ; Cattle Diseases - drug therapy ; Cattle Diseases - parasitology ; Chromatography, High Pressure Liquid - veterinary ; Fasciola hepatica ; Fasciola hepatica - drug effects ; Fasciola hepatica - embryology ; Fascioliasis - drug therapy ; Fascioliasis - parasitology ; Fascioliasis - veterinary ; Feces - parasitology ; Female ; Flavonoid ; Flavonoids - chemistry ; Flavonoids - isolation & purification ; Flavonoids - pharmacology ; Momordica charantia - chemistry ; Momordica charantia L ; Ovum - drug effects ; Parasite Egg Count ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Plants, Medicinal ; Quercetin ; Quercetin - chemistry ; Quercetin - isolation & purification ; Quercetin - pharmacology</subject><ispartof>Veterinary parasitology, 2016-09, Vol.228, p.160-166</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-7f19d7de02aab40e59584885e1d5c6c4df10d479208837d8ee44b30f6deef9a93</citedby><cites>FETCH-LOGICAL-c408t-7f19d7de02aab40e59584885e1d5c6c4df10d479208837d8ee44b30f6deef9a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2016.08.025$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27692319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pereira, Cíntia A.J.</creatorcontrib><creatorcontrib>Oliveira, Laura L.S.</creatorcontrib><creatorcontrib>Coaglio, Aytube L.</creatorcontrib><creatorcontrib>Santos, Fernanda S.O.</creatorcontrib><creatorcontrib>Cezar, Rodolfo S.M.</creatorcontrib><creatorcontrib>Mendes, Tiago</creatorcontrib><creatorcontrib>Oliveira, Fernando L.P.</creatorcontrib><creatorcontrib>Conzensa, Gustavo</creatorcontrib><creatorcontrib>Lima, Walter S.</creatorcontrib><title>Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>[Display omitted]
•Momordica charantia prevent embryogenesis in Fasciola hepatica eggs.•Anti-ovicidal activity against F. hepatica of the flavonoid quercetin M. charantia.•Momordica charantia is a potential alternative to control helminth parasites.
Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid–liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC–MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions.</description><subject>Albendazole - analogs & derivatives</subject><subject>Albendazole - pharmacology</subject><subject>Animals</subject><subject>Anthelmintics - pharmacology</subject><subject>Anti-ovicidal activity</subject><subject>Cattle</subject><subject>Cattle Diseases - drug therapy</subject><subject>Cattle Diseases - parasitology</subject><subject>Chromatography, High Pressure Liquid - veterinary</subject><subject>Fasciola hepatica</subject><subject>Fasciola hepatica - drug effects</subject><subject>Fasciola hepatica - embryology</subject><subject>Fascioliasis - drug therapy</subject><subject>Fascioliasis - parasitology</subject><subject>Fascioliasis - veterinary</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Flavonoid</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - isolation & purification</subject><subject>Flavonoids - pharmacology</subject><subject>Momordica charantia - chemistry</subject><subject>Momordica charantia L</subject><subject>Ovum - drug effects</subject><subject>Parasite Egg Count</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Plants, Medicinal</subject><subject>Quercetin</subject><subject>Quercetin - chemistry</subject><subject>Quercetin - isolation & purification</subject><subject>Quercetin - pharmacology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGO0zAQhi0EYsvCGyDkI5eEceLEzgVptdoFpCIucLam9qR1lcTFdovK0-OqC0dOMyN9M7_mY-ytgFqA6D_s6xPlA8a6KVMNuoame8ZWQqu2aroOnrMVtCArCULdsFcp7QFAQq9esptG9UPTimHFft8t2Vc7mma_5J23HG32J5_PPIz8a5hDdN4itzuMWEjk65rjFv2SMn_EZH2YkO_ogPmC0XabOI6ZIs-_aDoRd3hOl1N-scdNgcJSWl4CYnjNXow4JXrzVG_Zj8eH7_efq_W3T1_u79aVlaBzpUYxOOUIGsSNBOqGTkutOxKus72VbhTgpBoa0LpVThNJuWlh7B3ROODQ3rL317uHGH4eKWUz-2RpmnChcExG6LZrB2gUFFReURtDSpFGc4h-xng2AszFutmbq3VzsW5Am2K9rL17SjhuZnL_lv5qLsDHK0Dlz5OnaIo5Wiw5H8lm44L_f8IfZMeXIQ</recordid><startdate>20160915</startdate><enddate>20160915</enddate><creator>Pereira, Cíntia A.J.</creator><creator>Oliveira, Laura L.S.</creator><creator>Coaglio, Aytube L.</creator><creator>Santos, Fernanda S.O.</creator><creator>Cezar, Rodolfo S.M.</creator><creator>Mendes, Tiago</creator><creator>Oliveira, Fernando L.P.</creator><creator>Conzensa, Gustavo</creator><creator>Lima, Walter S.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160915</creationdate><title>Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro</title><author>Pereira, Cíntia A.J. ; Oliveira, Laura L.S. ; Coaglio, Aytube L. ; Santos, Fernanda S.O. ; Cezar, Rodolfo S.M. ; Mendes, Tiago ; Oliveira, Fernando L.P. ; Conzensa, Gustavo ; Lima, Walter S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-7f19d7de02aab40e59584885e1d5c6c4df10d479208837d8ee44b30f6deef9a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Albendazole - analogs & derivatives</topic><topic>Albendazole - pharmacology</topic><topic>Animals</topic><topic>Anthelmintics - pharmacology</topic><topic>Anti-ovicidal activity</topic><topic>Cattle</topic><topic>Cattle Diseases - drug therapy</topic><topic>Cattle Diseases - parasitology</topic><topic>Chromatography, High Pressure Liquid - veterinary</topic><topic>Fasciola hepatica</topic><topic>Fasciola hepatica - drug effects</topic><topic>Fasciola hepatica - embryology</topic><topic>Fascioliasis - drug therapy</topic><topic>Fascioliasis - parasitology</topic><topic>Fascioliasis - veterinary</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Flavonoid</topic><topic>Flavonoids - chemistry</topic><topic>Flavonoids - isolation & purification</topic><topic>Flavonoids - pharmacology</topic><topic>Momordica charantia - chemistry</topic><topic>Momordica charantia L</topic><topic>Ovum - drug effects</topic><topic>Parasite Egg Count</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Plants, Medicinal</topic><topic>Quercetin</topic><topic>Quercetin - chemistry</topic><topic>Quercetin - isolation & purification</topic><topic>Quercetin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, Cíntia A.J.</creatorcontrib><creatorcontrib>Oliveira, Laura L.S.</creatorcontrib><creatorcontrib>Coaglio, Aytube L.</creatorcontrib><creatorcontrib>Santos, Fernanda S.O.</creatorcontrib><creatorcontrib>Cezar, Rodolfo S.M.</creatorcontrib><creatorcontrib>Mendes, Tiago</creatorcontrib><creatorcontrib>Oliveira, Fernando L.P.</creatorcontrib><creatorcontrib>Conzensa, Gustavo</creatorcontrib><creatorcontrib>Lima, Walter S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, Cíntia A.J.</au><au>Oliveira, Laura L.S.</au><au>Coaglio, Aytube L.</au><au>Santos, Fernanda S.O.</au><au>Cezar, Rodolfo S.M.</au><au>Mendes, Tiago</au><au>Oliveira, Fernando L.P.</au><au>Conzensa, Gustavo</au><au>Lima, Walter S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2016-09-15</date><risdate>2016</risdate><volume>228</volume><spage>160</spage><epage>166</epage><pages>160-166</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>[Display omitted]
•Momordica charantia prevent embryogenesis in Fasciola hepatica eggs.•Anti-ovicidal activity against F. hepatica of the flavonoid quercetin M. charantia.•Momordica charantia is a potential alternative to control helminth parasites.
Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid–liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC–MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27692319</pmid><doi>10.1016/j.vetpar.2016.08.025</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Albendazole - analogs & derivatives Albendazole - pharmacology Animals Anthelmintics - pharmacology Anti-ovicidal activity Cattle Cattle Diseases - drug therapy Cattle Diseases - parasitology Chromatography, High Pressure Liquid - veterinary Fasciola hepatica Fasciola hepatica - drug effects Fasciola hepatica - embryology Fascioliasis - drug therapy Fascioliasis - parasitology Fascioliasis - veterinary Feces - parasitology Female Flavonoid Flavonoids - chemistry Flavonoids - isolation & purification Flavonoids - pharmacology Momordica charantia - chemistry Momordica charantia L Ovum - drug effects Parasite Egg Count Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Leaves - chemistry Plants, Medicinal Quercetin Quercetin - chemistry Quercetin - isolation & purification Quercetin - pharmacology |
title | Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro |
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