The main pigment of the dormant Mycobacterium smegmatis is porphyrin
Upon transition of Mycobacterium smegmatis into the dormant state, accumulation of a dark brown fluorescent pigment was observed. This pigment gave bright red fluorescence in both cells and the culture medium. Based on 1H-NMR, MALDI and UV spectra, the fluorescent compounds, extracted from the cultu...
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| Veröffentlicht in: | FEMS microbiology letters 2016-10, Vol.363 (19), p.fnw206 |
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| container_title | FEMS microbiology letters |
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| creator | Nikitushkin, V. D. Shleeva, M. O. Zinin, A. I. Trutneva, K. A. Ostrovsky, D. N. Kaprelyants, A. S. |
| description | Upon transition of Mycobacterium smegmatis into the dormant state, accumulation of a dark brown fluorescent pigment was observed. This pigment gave bright red fluorescence in both cells and the culture medium. Based on 1H-NMR, MALDI and UV spectra, the fluorescent compounds, extracted from the culture medium as well as from the dormant cells, were concluded to be a mixture of free coproporphyrin III and uroporphyrin III and their corresponding methyl esters. A possible significance of porphyrin pigment accumulation in the dormant cells is discussed.
The main pigment of the dormant Mycobacterium smegmatis is porphyrin. |
| doi_str_mv | 10.1093/femsle/fnw206 |
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The main pigment of the dormant Mycobacterium smegmatis is porphyrin.</description><subject>Accumulation</subject><subject>Cell culture</subject><subject>Chemical compounds</subject><subject>Coproporphyrins - chemistry</subject><subject>Coproporphyrins - isolation & purification</subject><subject>Culture Media - chemistry</subject><subject>Esters</subject><subject>Fluorescence</subject><subject>Gram-positive bacteria</subject><subject>Microbiology</subject><subject>Mycobacterium smegmatis</subject><subject>Mycobacterium smegmatis - chemistry</subject><subject>Mycobacterium smegmatis - physiology</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Pigments</subject><subject>Pigments, Biological - chemistry</subject><subject>Pigments, Biological - isolation & purification</subject><subject>Porphyrins - chemistry</subject><subject>Porphyrins - isolation & purification</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Uroporphyrins - chemistry</subject><subject>Uroporphyrins - isolation & purification</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkM1LwzAYh4Mobk6PXqXgxUtdmqRNcpT5CRMv8xzaNNkymqYmLbL_3kjnB16EF94PHn68PACcZ_A6gxzPtbKhUXPdviNYHIBpllOSFrxgh7_mCTgJYQshJBE6BhNEC8gRxlNwu9qoxJamTTqztqrtE6eTPt5q520Z1-eddFUpe-XNYJNg1dqWvQlJrM75brPzpj0FR7psgjrb9xl4vb9bLR7T5cvD0-JmmUrMWZ8iLhmrSS0pQ4QxrQvCagkxQoRiCiknutKMy0plqCJEopoXWmtSRETTSuIZuBpzO-_eBhV6YU2QqmnKVrkhiIzhHDMMcxbRyz_o1g2-jd8JBGGO8zznOFLpSEnvQvBKi84bW_qdyKD41CtGvWLUG_mLfepQWVV_018-fz50Q_dP1gc_mIT6</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Nikitushkin, V. 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D.</au><au>Shleeva, M. O.</au><au>Zinin, A. I.</au><au>Trutneva, K. A.</au><au>Ostrovsky, D. N.</au><au>Kaprelyants, A. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The main pigment of the dormant Mycobacterium smegmatis is porphyrin</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>363</volume><issue>19</issue><spage>fnw206</spage><pages>fnw206-</pages><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Upon transition of Mycobacterium smegmatis into the dormant state, accumulation of a dark brown fluorescent pigment was observed. This pigment gave bright red fluorescence in both cells and the culture medium. Based on 1H-NMR, MALDI and UV spectra, the fluorescent compounds, extracted from the culture medium as well as from the dormant cells, were concluded to be a mixture of free coproporphyrin III and uroporphyrin III and their corresponding methyl esters. A possible significance of porphyrin pigment accumulation in the dormant cells is discussed.
The main pigment of the dormant Mycobacterium smegmatis is porphyrin.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27609233</pmid><doi>10.1093/femsle/fnw206</doi></addata></record> |
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| source | Oxford University Press Journals; MEDLINE; Alma/SFX Local Collection |
| subjects | Accumulation Cell culture Chemical compounds Coproporphyrins - chemistry Coproporphyrins - isolation & purification Culture Media - chemistry Esters Fluorescence Gram-positive bacteria Microbiology Mycobacterium smegmatis Mycobacterium smegmatis - chemistry Mycobacterium smegmatis - physiology NMR Nuclear magnetic resonance Pigments Pigments, Biological - chemistry Pigments, Biological - isolation & purification Porphyrins - chemistry Porphyrins - isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Uroporphyrins - chemistry Uroporphyrins - isolation & purification |
| title | The main pigment of the dormant Mycobacterium smegmatis is porphyrin |
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