Toxoplasma gondii infection in dairy ewes: Vertical transmission and influence on milk production and reproductive performance
The present study aimed to evaluate the transmission of toxoplasmosis (vertical and venereal) and its influence on milk production and reproductive problems of Lacaune sheep seropositives for Toxoplasma gondii. Males and females were serologically selected using indirect immunofluorescence method in...
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| Veröffentlicht in: | Microbial pathogenesis 2016-10, Vol.99, p.101-105 |
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| creator | Klauck, Vanderlei Pazinato, Rafael Radavelli, Willian M. Custódio, Edimar Bianchi, Anderson E. Camillo, Giovana Cezar, Alfredo S. Vogel, Fernanda F. Tonin, Alexandre A. Ferreira, Rogério Stefani, Lenita M. Da Silva, Aleksandro S. |
| description | The present study aimed to evaluate the transmission of toxoplasmosis (vertical and venereal) and its influence on milk production and reproductive problems of Lacaune sheep seropositives for Toxoplasma gondii. Males and females were serologically selected using indirect immunofluorescence method in three steps of the study. Step 1: In order to evaluate the influence of toxoplasmosis on milk production, the volume of milk produced by 40 sheep (22 seronegatives and 18 seropositives for T. gondii) was weekly measured throughout the lactation period. There were no significant differences between these two groups; in other words, toxoplasmosis did not affect milk production. Step 2: In order to assess T. gondii venereal transmission, five samples of semen from seropositive rams (n = 5) were tested by endpoint and real time PCR with two days of interval; however, these semen samples were PCR negatives for T. gondii. Step 3: To evaluate reproductive problems, 12 seropositive animals out of a flock of 68 pregnant ewes showed signs of reproductive problems, such as abortion or fetal resorption. T. gondii transplacental transmission was evaluated on blood drawn from newborn lambs (n = 41), and their respective seropositive mothers (n = 30) after single, double or triple births. Serological tests showed that 65.8% of the lambs had antibodies against this protozoan, indicating a high transmission from ewe to fetus during pregnancy. Therefore, it is concluded that toxoplasmosis in sheep may impair reproduction with a high percentage of vertical transmission.
•Infection by Toxoplasma gondii adversely affects sheep, causing reproductive failure.•Vertical transmission of the parasite is high in Lacaune ewes.•Ewes seropositive for T. gondii have no affected its production of milk.•Seropositive rams (natural infection) has no parasite in semen, different experimental cases. |
| doi_str_mv | 10.1016/j.micpath.2016.08.012 |
| format | Article |
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•Infection by Toxoplasma gondii adversely affects sheep, causing reproductive failure.•Vertical transmission of the parasite is high in Lacaune ewes.•Ewes seropositive for T. gondii have no affected its production of milk.•Seropositive rams (natural infection) has no parasite in semen, different experimental cases.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2016.08.012</identifier><identifier>PMID: 27531118</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Female ; Infectious Disease Transmission, Vertical ; Lactation ; Male ; Milk - metabolism ; Reproduction ; Reproductive problems ; Semen - parasitology ; Sheep ; Sheep Diseases - epidemiology ; Sheep Diseases - transmission ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; Toxoplasmosis ; Toxoplasmosis, Animal - epidemiology ; Toxoplasmosis, Animal - transmission</subject><ispartof>Microbial pathogenesis, 2016-10, Vol.99, p.101-105</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-9607f03b1e9fe4d7cf9737dbb7c0fb604cc1d7f009f0d9fa83a4796f017a3d743</citedby><cites>FETCH-LOGICAL-c398t-9607f03b1e9fe4d7cf9737dbb7c0fb604cc1d7f009f0d9fa83a4796f017a3d743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0882401016304053$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27531118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klauck, Vanderlei</creatorcontrib><creatorcontrib>Pazinato, Rafael</creatorcontrib><creatorcontrib>Radavelli, Willian M.</creatorcontrib><creatorcontrib>Custódio, Edimar</creatorcontrib><creatorcontrib>Bianchi, Anderson E.</creatorcontrib><creatorcontrib>Camillo, Giovana</creatorcontrib><creatorcontrib>Cezar, Alfredo S.</creatorcontrib><creatorcontrib>Vogel, Fernanda F.</creatorcontrib><creatorcontrib>Tonin, Alexandre A.</creatorcontrib><creatorcontrib>Ferreira, Rogério</creatorcontrib><creatorcontrib>Stefani, Lenita M.</creatorcontrib><creatorcontrib>Da Silva, Aleksandro S.</creatorcontrib><title>Toxoplasma gondii infection in dairy ewes: Vertical transmission and influence on milk production and reproductive performance</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The present study aimed to evaluate the transmission of toxoplasmosis (vertical and venereal) and its influence on milk production and reproductive problems of Lacaune sheep seropositives for Toxoplasma gondii. Males and females were serologically selected using indirect immunofluorescence method in three steps of the study. Step 1: In order to evaluate the influence of toxoplasmosis on milk production, the volume of milk produced by 40 sheep (22 seronegatives and 18 seropositives for T. gondii) was weekly measured throughout the lactation period. There were no significant differences between these two groups; in other words, toxoplasmosis did not affect milk production. Step 2: In order to assess T. gondii venereal transmission, five samples of semen from seropositive rams (n = 5) were tested by endpoint and real time PCR with two days of interval; however, these semen samples were PCR negatives for T. gondii. Step 3: To evaluate reproductive problems, 12 seropositive animals out of a flock of 68 pregnant ewes showed signs of reproductive problems, such as abortion or fetal resorption. T. gondii transplacental transmission was evaluated on blood drawn from newborn lambs (n = 41), and their respective seropositive mothers (n = 30) after single, double or triple births. Serological tests showed that 65.8% of the lambs had antibodies against this protozoan, indicating a high transmission from ewe to fetus during pregnancy. Therefore, it is concluded that toxoplasmosis in sheep may impair reproduction with a high percentage of vertical transmission.
•Infection by Toxoplasma gondii adversely affects sheep, causing reproductive failure.•Vertical transmission of the parasite is high in Lacaune ewes.•Ewes seropositive for T. gondii have no affected its production of milk.•Seropositive rams (natural infection) has no parasite in semen, different experimental cases.</description><subject>Animals</subject><subject>Female</subject><subject>Infectious Disease Transmission, Vertical</subject><subject>Lactation</subject><subject>Male</subject><subject>Milk - metabolism</subject><subject>Reproduction</subject><subject>Reproductive problems</subject><subject>Semen - parasitology</subject><subject>Sheep</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - transmission</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Toxoplasmosis, Animal - transmission</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1TAQQC1ERW8LnwDykk3COE5imw1CFVCkSmzabi3HHoMveWEnpd3023F0b9nCyuPRmYfmEPKaQcmAte_25RDsbJYfZZW_JcgSWPWM7BiotmAVyOdkB1JWRQ0MTslZSnsAUDVXL8hpJRrOGJM78ng93U9zb9Jg6PdpdCHQMHq0S5jGHFFnQnyg-BvTe3qLcQnW9HSJZkxDSGmDzOi2kn7F0SLNiSH0P-kcJ7ceumxAxKfEHdIZo5_iYDL_kpx40yd8dXzPyc3nT9cXl8XVty9fLz5eFZYruRSqBeGBdwyVx9oJ65XgwnWdsOC7FmprmcsEKA9OeSO5qYVqPTBhuBM1PydvD33zGr9WTIvO61vsezPitCbNJG-4aGTD_wetqroRIDLaHFAbp5Qiej3HMJj4oBnozZLe66MlvVnSIHW2lOveHEes3YDub9WTlgx8OACYb3IXMOpkw3ZfF2J2o90U_jHiD3iUqOM</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Klauck, Vanderlei</creator><creator>Pazinato, Rafael</creator><creator>Radavelli, Willian M.</creator><creator>Custódio, Edimar</creator><creator>Bianchi, Anderson E.</creator><creator>Camillo, Giovana</creator><creator>Cezar, Alfredo S.</creator><creator>Vogel, Fernanda F.</creator><creator>Tonin, Alexandre A.</creator><creator>Ferreira, Rogério</creator><creator>Stefani, Lenita M.</creator><creator>Da Silva, Aleksandro S.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Toxoplasma gondii infection in dairy ewes: Vertical transmission and influence on milk production and reproductive performance</title><author>Klauck, Vanderlei ; Pazinato, Rafael ; Radavelli, Willian M. ; Custódio, Edimar ; Bianchi, Anderson E. ; Camillo, Giovana ; Cezar, Alfredo S. ; Vogel, Fernanda F. ; Tonin, Alexandre A. ; Ferreira, Rogério ; Stefani, Lenita M. ; Da Silva, Aleksandro S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-9607f03b1e9fe4d7cf9737dbb7c0fb604cc1d7f009f0d9fa83a4796f017a3d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Female</topic><topic>Infectious Disease Transmission, Vertical</topic><topic>Lactation</topic><topic>Male</topic><topic>Milk - metabolism</topic><topic>Reproduction</topic><topic>Reproductive problems</topic><topic>Semen - parasitology</topic><topic>Sheep</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - transmission</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Toxoplasmosis, Animal - transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klauck, Vanderlei</creatorcontrib><creatorcontrib>Pazinato, Rafael</creatorcontrib><creatorcontrib>Radavelli, Willian M.</creatorcontrib><creatorcontrib>Custódio, Edimar</creatorcontrib><creatorcontrib>Bianchi, Anderson E.</creatorcontrib><creatorcontrib>Camillo, Giovana</creatorcontrib><creatorcontrib>Cezar, Alfredo S.</creatorcontrib><creatorcontrib>Vogel, Fernanda F.</creatorcontrib><creatorcontrib>Tonin, Alexandre A.</creatorcontrib><creatorcontrib>Ferreira, Rogério</creatorcontrib><creatorcontrib>Stefani, Lenita M.</creatorcontrib><creatorcontrib>Da Silva, Aleksandro S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klauck, Vanderlei</au><au>Pazinato, Rafael</au><au>Radavelli, Willian M.</au><au>Custódio, Edimar</au><au>Bianchi, Anderson E.</au><au>Camillo, Giovana</au><au>Cezar, Alfredo S.</au><au>Vogel, Fernanda F.</au><au>Tonin, Alexandre A.</au><au>Ferreira, Rogério</au><au>Stefani, Lenita M.</au><au>Da Silva, Aleksandro S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxoplasma gondii infection in dairy ewes: Vertical transmission and influence on milk production and reproductive performance</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2016-10</date><risdate>2016</risdate><volume>99</volume><spage>101</spage><epage>105</epage><pages>101-105</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>The present study aimed to evaluate the transmission of toxoplasmosis (vertical and venereal) and its influence on milk production and reproductive problems of Lacaune sheep seropositives for Toxoplasma gondii. Males and females were serologically selected using indirect immunofluorescence method in three steps of the study. Step 1: In order to evaluate the influence of toxoplasmosis on milk production, the volume of milk produced by 40 sheep (22 seronegatives and 18 seropositives for T. gondii) was weekly measured throughout the lactation period. There were no significant differences between these two groups; in other words, toxoplasmosis did not affect milk production. Step 2: In order to assess T. gondii venereal transmission, five samples of semen from seropositive rams (n = 5) were tested by endpoint and real time PCR with two days of interval; however, these semen samples were PCR negatives for T. gondii. Step 3: To evaluate reproductive problems, 12 seropositive animals out of a flock of 68 pregnant ewes showed signs of reproductive problems, such as abortion or fetal resorption. T. gondii transplacental transmission was evaluated on blood drawn from newborn lambs (n = 41), and their respective seropositive mothers (n = 30) after single, double or triple births. Serological tests showed that 65.8% of the lambs had antibodies against this protozoan, indicating a high transmission from ewe to fetus during pregnancy. Therefore, it is concluded that toxoplasmosis in sheep may impair reproduction with a high percentage of vertical transmission.
•Infection by Toxoplasma gondii adversely affects sheep, causing reproductive failure.•Vertical transmission of the parasite is high in Lacaune ewes.•Ewes seropositive for T. gondii have no affected its production of milk.•Seropositive rams (natural infection) has no parasite in semen, different experimental cases.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27531118</pmid><doi>10.1016/j.micpath.2016.08.012</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Female Infectious Disease Transmission, Vertical Lactation Male Milk - metabolism Reproduction Reproductive problems Semen - parasitology Sheep Sheep Diseases - epidemiology Sheep Diseases - transmission Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis Toxoplasmosis, Animal - epidemiology Toxoplasmosis, Animal - transmission |
| title | Toxoplasma gondii infection in dairy ewes: Vertical transmission and influence on milk production and reproductive performance |
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