Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer
Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (...
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description | Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains, suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs. |
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Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains, suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs.</description><identifier>ISSN: 2046-2441</identifier><identifier>EISSN: 2046-2441</identifier><identifier>DOI: 10.1098/rsob.160084</identifier><identifier>PMID: 27707895</identifier><language>eng</language><publisher>England: The Royal Society</publisher><subject>Bacterial Proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Chromosomes, Bacterial ; Chromosomes, Bacterial - genetics ; Cloning, Molecular ; Cloning, Molecular - methods ; Conjugation ; DNA Nucleotidyltransferases ; DNA Nucleotidyltransferases - genetics ; DNA Nucleotidyltransferases - metabolism ; Gene Transfer, Horizontal ; Genetic Variation ; Horizontal Gene Transfer ; Integrative And Mobilizable Elements Mobilization ; Interspersed Repetitive Sequences ; Life Sciences ; Open Reading Frames ; Relaxases ; Ribosomal Proteins ; Ribosomal Proteins - genetics ; Sequence Analysis, DNA ; streptococcus ; Streptococcus agalactiae ; Streptococcus agalactiae - enzymology ; Streptococcus agalactiae - genetics</subject><ispartof>Open biology, 2016-10, Vol.6 (10), p.160084</ispartof><rights>2016 The Authors.</rights><rights>Attribution</rights><rights>2016 The Authors. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-54000b1b2db017cbd0c8cb194ffad8c31c3d546c54218889ebc91130c06af5733</citedby><cites>FETCH-LOGICAL-c489t-54000b1b2db017cbd0c8cb194ffad8c31c3d546c54218889ebc91130c06af5733</cites><orcidid>0000-0003-3958-0543 ; 0000-0003-4156-2782 ; 0000-0001-5352-069X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090054/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090054/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,3322,27147,27924,27925,53791,53793,55555,55565</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27707895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-03108010$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Lorenzo-Diaz, Fabian</creatorcontrib><creatorcontrib>Fernández-Lopez, Cris</creatorcontrib><creatorcontrib>Douarre, Pierre-Emmanuel</creatorcontrib><creatorcontrib>Baez-Ortega, Adrian</creatorcontrib><creatorcontrib>Flores, Carlos</creatorcontrib><creatorcontrib>Glaser, Philippe</creatorcontrib><creatorcontrib>Espinosa, Manuel</creatorcontrib><title>Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer</title><title>Open biology</title><addtitle>Open Biol</addtitle><addtitle>Open Biol</addtitle><description>Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains, suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs.</description><subject>Bacterial Proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Chromosomes, Bacterial</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Cloning, Molecular</subject><subject>Cloning, Molecular - methods</subject><subject>Conjugation</subject><subject>DNA Nucleotidyltransferases</subject><subject>DNA Nucleotidyltransferases - genetics</subject><subject>DNA Nucleotidyltransferases - metabolism</subject><subject>Gene Transfer, Horizontal</subject><subject>Genetic Variation</subject><subject>Horizontal Gene Transfer</subject><subject>Integrative And Mobilizable Elements Mobilization</subject><subject>Interspersed Repetitive Sequences</subject><subject>Life Sciences</subject><subject>Open Reading Frames</subject><subject>Relaxases</subject><subject>Ribosomal Proteins</subject><subject>Ribosomal Proteins - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>streptococcus</subject><subject>Streptococcus agalactiae</subject><subject>Streptococcus agalactiae - enzymology</subject><subject>Streptococcus agalactiae - genetics</subject><issn>2046-2441</issn><issn>2046-2441</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNptksFvFCEUxidGY5vak3fD0cRMhYGZgYtJbWq7yRoP1TNhmMeWDTuMwEy6xj9etlOb1siFF_j4Pd57X1G8JfiMYME_hui7M9JgzNmL4rjCrCkrxsjLJ_FRcRrjFudVN0Qw8ro4qtoWt1zUx8XvmxRgTF57rZVDm-CnEX1GdkiwCSrZGZAaerTznXX2l-ocIHCwgyGh1dfLG7UpwxhXCAbte4hIITMNOlk_ZFgAp-5UhEybvZuhzwGyKaIU1BANhDfFK6NchNOH_aT48eXy-8V1uf52tbo4X5eacZHKmuW_d6Sr-g6TVnc91lx3uRRjVM81JZr2NWt0zSrCORfQaUEIxRo3ytQtpSfFauH2Xm3lGOxOhb30ysr7Ax82UoVktQNZ564JmtvZUsOMIoI2TFDCemUMZ4Jl1qeFNU7dDnqdOxGUewZ9fjPYW7nxcyaLPIEDoFwAt_88uz5fy1HFBFOQmBLMMcEzyfr3DwmD_zlBTHJnowbn1AB-ipJwWtOW8Qpn6YdFqoOPMYB55BMsD3aRB7vIxS5Z_e5pJY_av-bIArwIgt_n8XhtIe3l1k8hTzf-l_kHw9_NDg</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Lorenzo-Diaz, Fabian</creator><creator>Fernández-Lopez, Cris</creator><creator>Douarre, Pierre-Emmanuel</creator><creator>Baez-Ortega, Adrian</creator><creator>Flores, Carlos</creator><creator>Glaser, Philippe</creator><creator>Espinosa, Manuel</creator><general>The Royal Society</general><general>Royal Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3958-0543</orcidid><orcidid>https://orcid.org/0000-0003-4156-2782</orcidid><orcidid>https://orcid.org/0000-0001-5352-069X</orcidid></search><sort><creationdate>20161001</creationdate><title>Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer</title><author>Lorenzo-Diaz, Fabian ; Fernández-Lopez, Cris ; Douarre, Pierre-Emmanuel ; Baez-Ortega, Adrian ; Flores, Carlos ; Glaser, Philippe ; Espinosa, Manuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-54000b1b2db017cbd0c8cb194ffad8c31c3d546c54218889ebc91130c06af5733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacterial Proteins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Chromosomes, Bacterial</topic><topic>Chromosomes, Bacterial - genetics</topic><topic>Cloning, Molecular</topic><topic>Cloning, Molecular - methods</topic><topic>Conjugation</topic><topic>DNA Nucleotidyltransferases</topic><topic>DNA Nucleotidyltransferases - genetics</topic><topic>DNA Nucleotidyltransferases - metabolism</topic><topic>Gene Transfer, Horizontal</topic><topic>Genetic Variation</topic><topic>Horizontal Gene Transfer</topic><topic>Integrative And Mobilizable Elements Mobilization</topic><topic>Interspersed Repetitive Sequences</topic><topic>Life Sciences</topic><topic>Open Reading Frames</topic><topic>Relaxases</topic><topic>Ribosomal Proteins</topic><topic>Ribosomal Proteins - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>streptococcus</topic><topic>Streptococcus agalactiae</topic><topic>Streptococcus agalactiae - enzymology</topic><topic>Streptococcus agalactiae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lorenzo-Diaz, Fabian</creatorcontrib><creatorcontrib>Fernández-Lopez, Cris</creatorcontrib><creatorcontrib>Douarre, Pierre-Emmanuel</creatorcontrib><creatorcontrib>Baez-Ortega, Adrian</creatorcontrib><creatorcontrib>Flores, Carlos</creatorcontrib><creatorcontrib>Glaser, Philippe</creatorcontrib><creatorcontrib>Espinosa, Manuel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Open biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lorenzo-Diaz, Fabian</au><au>Fernández-Lopez, Cris</au><au>Douarre, Pierre-Emmanuel</au><au>Baez-Ortega, Adrian</au><au>Flores, Carlos</au><au>Glaser, Philippe</au><au>Espinosa, Manuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer</atitle><jtitle>Open biology</jtitle><stitle>Open Biol</stitle><addtitle>Open Biol</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>6</volume><issue>10</issue><spage>160084</spage><pages>160084-</pages><issn>2046-2441</issn><eissn>2046-2441</eissn><abstract>Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 3′-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains, suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs.</abstract><cop>England</cop><pub>The Royal Society</pub><pmid>27707895</pmid><doi>10.1098/rsob.160084</doi><orcidid>https://orcid.org/0000-0003-3958-0543</orcidid><orcidid>https://orcid.org/0000-0003-4156-2782</orcidid><orcidid>https://orcid.org/0000-0001-5352-069X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Bacterial Proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Chromosomes, Bacterial Chromosomes, Bacterial - genetics Cloning, Molecular Cloning, Molecular - methods Conjugation DNA Nucleotidyltransferases DNA Nucleotidyltransferases - genetics DNA Nucleotidyltransferases - metabolism Gene Transfer, Horizontal Genetic Variation Horizontal Gene Transfer Integrative And Mobilizable Elements Mobilization Interspersed Repetitive Sequences Life Sciences Open Reading Frames Relaxases Ribosomal Proteins Ribosomal Proteins - genetics Sequence Analysis, DNA streptococcus Streptococcus agalactiae Streptococcus agalactiae - enzymology Streptococcus agalactiae - genetics |
title | Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer |
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