Nitrification rates in a saline medium at different dissolved oxygen concentrations

A kinetic study was carried out in a saline medium to assess the effect of O^sub 2^ on the two-step nitrification process: for the first nitritation step, 2-26 mg dissolved O^sub 2^ (DO) l^sup -1^ was used and for the second nitratation step, 0.5-24 mg DO l^sup -1^ was used. Nitritation rate was mea...

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Veröffentlicht in:Biotechnology letters 2001-10, Vol.23 (19), p.1597-1602
Hauptverfasser: SANCHEZ, Omar, MARTI, Maria C, ASPC, Estrella, ROECKEL, Marlene
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container_end_page 1602
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container_title Biotechnology letters
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creator SANCHEZ, Omar
MARTI, Maria C
ASPC, Estrella
ROECKEL, Marlene
description A kinetic study was carried out in a saline medium to assess the effect of O^sub 2^ on the two-step nitrification process: for the first nitritation step, 2-26 mg dissolved O^sub 2^ (DO) l^sup -1^ was used and for the second nitratation step, 0.5-24 mg DO l^sup -1^ was used. Nitritation rate was measured in the presence of sodium azide so as to inhibit nitratation. Ammonia-oxidizing (AOB) and nitrite-oxidizing (NOB) bacterial in the mixed culture were determined by 16 S rRNA hybridization. The affinity constants for oxygen O^sub 2^ of the AOB and the NOB were 1.66 mg O^sub 2^ l^sup -1^ and 3 mg O^sub 2^ l^sup -1^ respectively. The larger than the previously reported values of these constants might be due to the high salt content in the medium. High O^sub 2^ concentrations did inhibit the nitrification rate.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/A:1011977629398
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Nitritation rate was measured in the presence of sodium azide so as to inhibit nitratation. Ammonia-oxidizing (AOB) and nitrite-oxidizing (NOB) bacterial in the mixed culture were determined by 16 S rRNA hybridization. The affinity constants for oxygen O^sub 2^ of the AOB and the NOB were 1.66 mg O^sub 2^ l^sup -1^ and 3 mg O^sub 2^ l^sup -1^ respectively. The larger than the previously reported values of these constants might be due to the high salt content in the medium. 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subjects ammonia
Biological and medical sciences
Biological treatment of waters
Biotechnology
Dissolved oxygen
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Nitrification
rRNA 16S
sodium azide
title Nitrification rates in a saline medium at different dissolved oxygen concentrations
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