DIVERSITY OF THE TYPE 1 INTRON–ITS REGION OF THE 18S rRNA GENE IN PSEUDOGYMNOASCUS SPECIES FROM THE RED HILLS OF KANSAS
Gypsum caves found throughout the Red Hills of Kansas have the state's most diverse and largest population of cave-roosting bats. White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected i...
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| Veröffentlicht in: | Journal of zoo and wildlife medicine 2016-09, Vol.47 (3), p.883-885 |
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| container_title | Journal of zoo and wildlife medicine |
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| creator | Chen, Xi Crupper, Scott S |
| description | Gypsum caves found throughout the Red Hills of Kansas have the state's most diverse and largest population of cave-roosting bats. White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected in the gypsum caves as this disease moves westward from the eastern United States. Cave soil was obtained from the gypsum caves, and using the polymerase chain reaction, a 624-nucleotide DNA fragment specific to the Type 1 intron–internal transcribed spacer region of the 18S rRNA gene from Pseudogymnoascus species was amplified. Subsequent cloning and DNA sequencing indicated P. destructans DNA was present, along with 26 uncharacterized Pseudogymnoascus DNA variants. However, no evidence of WNS was observed in bat populations residing in these caves. |
| doi_str_mv | 10.1638/2015-0089.1 |
| format | Article |
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White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected in the gypsum caves as this disease moves westward from the eastern United States. Cave soil was obtained from the gypsum caves, and using the polymerase chain reaction, a 624-nucleotide DNA fragment specific to the Type 1 intron–internal transcribed spacer region of the 18S rRNA gene from Pseudogymnoascus species was amplified. Subsequent cloning and DNA sequencing indicated P. destructans DNA was present, along with 26 uncharacterized Pseudogymnoascus DNA variants. However, no evidence of WNS was observed in bat populations residing in these caves.</description><identifier>ISSN: 1042-7260</identifier><identifier>EISSN: 1937-2825</identifier><identifier>DOI: 10.1638/2015-0089.1</identifier><identifier>PMID: 27691955</identifier><language>eng</language><publisher>United States: American Association of Zoo Veterinarians</publisher><subject>Animals ; Ascomycota - genetics ; BRIEF COMMUNICATIONS ; Chiroptera - microbiology ; DNA, Fungal - genetics ; DNA, Ribosomal Spacer - genetics ; Geomyces destructans ; gypsum caves ; Kansas ; Pseudogymnoascus destructans ; Red Hills ; RNA, Fungal - genetics ; RNA, Ribosomal, 18S - genetics ; Soil Microbiology ; white-nose syndrome</subject><ispartof>Journal of zoo and wildlife medicine, 2016-09, Vol.47 (3), p.883-885</ispartof><rights>Copyright 2016 by American Association of Zoo Veterinarians</rights><rights>Copyright 2016 American Association of Zoo Veterinarians</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b303t-e56d905c690bd4164403c52f077022df4ac10b759f9079f27b9da3b2865ff5cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24773971$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24773971$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27691955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Crupper, Scott S</creatorcontrib><title>DIVERSITY OF THE TYPE 1 INTRON–ITS REGION OF THE 18S rRNA GENE IN PSEUDOGYMNOASCUS SPECIES FROM THE RED HILLS OF KANSAS</title><title>Journal of zoo and wildlife medicine</title><addtitle>J Zoo Wildl Med</addtitle><description>Gypsum caves found throughout the Red Hills of Kansas have the state's most diverse and largest population of cave-roosting bats. White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected in the gypsum caves as this disease moves westward from the eastern United States. Cave soil was obtained from the gypsum caves, and using the polymerase chain reaction, a 624-nucleotide DNA fragment specific to the Type 1 intron–internal transcribed spacer region of the 18S rRNA gene from Pseudogymnoascus species was amplified. Subsequent cloning and DNA sequencing indicated P. destructans DNA was present, along with 26 uncharacterized Pseudogymnoascus DNA variants. However, no evidence of WNS was observed in bat populations residing in these caves.</description><subject>Animals</subject><subject>Ascomycota - genetics</subject><subject>BRIEF COMMUNICATIONS</subject><subject>Chiroptera - microbiology</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Geomyces destructans</subject><subject>gypsum caves</subject><subject>Kansas</subject><subject>Pseudogymnoascus destructans</subject><subject>Red Hills</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Soil Microbiology</subject><subject>white-nose syndrome</subject><issn>1042-7260</issn><issn>1937-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1u2zAURomiRZ04nTq34NgiUHJJiqI4CjZtC7UlQ5QLeBL0R8CBHTmiPXjLO-QN8ySRaqfdOt0LfOd-FzgIfSVwRzzm31Mg3AHw5R35gK6IZMKhPuUfux1c6gjqwQBdW_sAQDxK3M9oQIUnieT8Cp3G4W-V6DBd43iC05nC6XqpMMFhlCZx9Pr8EqYaJ2oaxtE7QXyN2yQK8FRFqgPxUqvVOJ6uF1Ec6NFKY71Uo1BpPEnixZ-TRI3xLJzPdd_xK4h0oG_QJ5Nvbf3lModoNVHpaObM42k4CuZOwYAdnJp7lQReehKKyiWe6wIrOTUgBFBaGTcvCRSCSyNBSENFIaucFdT3uDG8NGyIfpx7923zdKztIdttbFlvt_lj3RxtRnzGGfelZB16e0bLtrG2rU22bze7vD1lBLLedda7znrXGeno75fiY7Grq7_su9wO-HYGHuyhaf_lrhBMir7g5zkvNk3zWP_32RtEdoTh</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Chen, Xi</creator><creator>Crupper, Scott S</creator><general>American Association of Zoo Veterinarians</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201609</creationdate><title>DIVERSITY OF THE TYPE 1 INTRON–ITS REGION OF THE 18S rRNA GENE IN PSEUDOGYMNOASCUS SPECIES FROM THE RED HILLS OF KANSAS</title><author>Chen, Xi ; Crupper, Scott S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b303t-e56d905c690bd4164403c52f077022df4ac10b759f9079f27b9da3b2865ff5cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Ascomycota - genetics</topic><topic>BRIEF COMMUNICATIONS</topic><topic>Chiroptera - microbiology</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Geomyces destructans</topic><topic>gypsum caves</topic><topic>Kansas</topic><topic>Pseudogymnoascus destructans</topic><topic>Red Hills</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Soil Microbiology</topic><topic>white-nose syndrome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Crupper, Scott S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of zoo and wildlife medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xi</au><au>Crupper, Scott S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DIVERSITY OF THE TYPE 1 INTRON–ITS REGION OF THE 18S rRNA GENE IN PSEUDOGYMNOASCUS SPECIES FROM THE RED HILLS OF KANSAS</atitle><jtitle>Journal of zoo and wildlife medicine</jtitle><addtitle>J Zoo Wildl Med</addtitle><date>2016-09</date><risdate>2016</risdate><volume>47</volume><issue>3</issue><spage>883</spage><epage>885</epage><pages>883-885</pages><issn>1042-7260</issn><eissn>1937-2825</eissn><abstract>Gypsum caves found throughout the Red Hills of Kansas have the state's most diverse and largest population of cave-roosting bats. White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected in the gypsum caves as this disease moves westward from the eastern United States. Cave soil was obtained from the gypsum caves, and using the polymerase chain reaction, a 624-nucleotide DNA fragment specific to the Type 1 intron–internal transcribed spacer region of the 18S rRNA gene from Pseudogymnoascus species was amplified. Subsequent cloning and DNA sequencing indicated P. destructans DNA was present, along with 26 uncharacterized Pseudogymnoascus DNA variants. However, no evidence of WNS was observed in bat populations residing in these caves.</abstract><cop>United States</cop><pub>American Association of Zoo Veterinarians</pub><pmid>27691955</pmid><doi>10.1638/2015-0089.1</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 1042-7260 |
| ispartof | Journal of zoo and wildlife medicine, 2016-09, Vol.47 (3), p.883-885 |
| issn | 1042-7260 1937-2825 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Animals Ascomycota - genetics BRIEF COMMUNICATIONS Chiroptera - microbiology DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics Geomyces destructans gypsum caves Kansas Pseudogymnoascus destructans Red Hills RNA, Fungal - genetics RNA, Ribosomal, 18S - genetics Soil Microbiology white-nose syndrome |
| title | DIVERSITY OF THE TYPE 1 INTRON–ITS REGION OF THE 18S rRNA GENE IN PSEUDOGYMNOASCUS SPECIES FROM THE RED HILLS OF KANSAS |
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