Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications
Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight a...
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| Veröffentlicht in: | International journal of biological macromolecules 2016-12, Vol.93 (Pt A), p.1133-1140 |
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| container_title | International journal of biological macromolecules |
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| creator | Lou, Ruyun Xie, Hongguo Zheng, Huizhen Ren, Ying Gao, Meng Guo, Xin Song, Yizhe Yu, Weiting Liu, Xiudong Ma, Xiaojun |
| description | Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte–matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system. |
| doi_str_mv | 10.1016/j.ijbiomac.2016.09.078 |
| format | Article |
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The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte–matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2016.09.078</identifier><identifier>PMID: 27667543</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alginates - chemistry ; Alginates - metabolism ; Alginates - pharmacology ; Animals ; Asialoglycoproteins - metabolism ; Biological Transport ; Capsules ; Cell Survival - drug effects ; Chitosan - chemistry ; Galactose - chemistry ; Galactosylated chitosan ; Glucuronic Acid - chemistry ; Glucuronic Acid - metabolism ; Glucuronic Acid - pharmacology ; Hepatocyte ; Hepatocytes - cytology ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Hexuronic Acids - chemistry ; Hexuronic Acids - metabolism ; Hexuronic Acids - pharmacology ; Male ; Mechanical Phenomena ; Microcapsules ; Molecular Weight ; Permeability ; Polylysine - chemistry ; Rats ; Rats, Sprague-Dawley ; Solubility ; Water - chemistry</subject><ispartof>International journal of biological macromolecules, 2016-12, Vol.93 (Pt A), p.1133-1140</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-6c931ae9979a26e7158b5fb7e262a45bbac7f7b0adf6df728bd4d0c1c11a516b3</citedby><cites>FETCH-LOGICAL-c368t-6c931ae9979a26e7158b5fb7e262a45bbac7f7b0adf6df728bd4d0c1c11a516b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2016.09.078$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27667543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lou, Ruyun</creatorcontrib><creatorcontrib>Xie, Hongguo</creatorcontrib><creatorcontrib>Zheng, Huizhen</creatorcontrib><creatorcontrib>Ren, Ying</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Guo, Xin</creatorcontrib><creatorcontrib>Song, Yizhe</creatorcontrib><creatorcontrib>Yu, Weiting</creatorcontrib><creatorcontrib>Liu, Xiudong</creatorcontrib><creatorcontrib>Ma, Xiaojun</creatorcontrib><title>Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte–matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system.</description><subject>Alginates - chemistry</subject><subject>Alginates - metabolism</subject><subject>Alginates - pharmacology</subject><subject>Animals</subject><subject>Asialoglycoproteins - metabolism</subject><subject>Biological Transport</subject><subject>Capsules</subject><subject>Cell Survival - drug effects</subject><subject>Chitosan - chemistry</subject><subject>Galactose - chemistry</subject><subject>Galactosylated chitosan</subject><subject>Glucuronic Acid - chemistry</subject><subject>Glucuronic Acid - metabolism</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Hepatocyte</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Hexuronic Acids - chemistry</subject><subject>Hexuronic Acids - metabolism</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Male</subject><subject>Mechanical Phenomena</subject><subject>Microcapsules</subject><subject>Molecular Weight</subject><subject>Permeability</subject><subject>Polylysine - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Solubility</subject><subject>Water - chemistry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vHCEMhlHVqtmk_QsRx15mAvMBzK1R1C8pUi_tGRnwZFkxwxTYVvvvS7SbXnuybD229T6E3HLWcsbF3aH1B-PjArbtat-yqWVSvSI7ruTUMMb612TH-MAbxXt2Ra5zPtSpGLl6S646KYQch35H5vvw5Fco2BjI6OjibYoWtnwMmOkfX_b0CQLYEvMpVMxRu_e1gZX6tWBaIdA5Jrolv0A60T1uUKI9FaSwbcFbKD6u-R15M0PI-P5Sb8jPz59-PHxtHr9_-fZw_9jYXqjSCDv1HHCa5ASdQMlHZcbZSOxEB8NoDFg5S8PAzcLNslPGDY5ZbjmHkQvT35AP57tbir-OmItefLYYAqwYj1lz1Y_92A1KVFSc0Ro454SzvmTQnOlnx_qgXxzrZ8eaTbo6rou3lx9Hs6D7t_YitQIfzwDWpL89Jp2tx9Wi8wlt0S76__34C8bilC8</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Lou, Ruyun</creator><creator>Xie, Hongguo</creator><creator>Zheng, Huizhen</creator><creator>Ren, Ying</creator><creator>Gao, Meng</creator><creator>Guo, Xin</creator><creator>Song, Yizhe</creator><creator>Yu, Weiting</creator><creator>Liu, Xiudong</creator><creator>Ma, Xiaojun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications</title><author>Lou, Ruyun ; Xie, Hongguo ; Zheng, Huizhen ; Ren, Ying ; Gao, Meng ; Guo, Xin ; Song, Yizhe ; Yu, Weiting ; Liu, Xiudong ; Ma, Xiaojun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-6c931ae9979a26e7158b5fb7e262a45bbac7f7b0adf6df728bd4d0c1c11a516b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Alginates - chemistry</topic><topic>Alginates - metabolism</topic><topic>Alginates - pharmacology</topic><topic>Animals</topic><topic>Asialoglycoproteins - metabolism</topic><topic>Biological Transport</topic><topic>Capsules</topic><topic>Cell Survival - drug effects</topic><topic>Chitosan - chemistry</topic><topic>Galactose - chemistry</topic><topic>Galactosylated chitosan</topic><topic>Glucuronic Acid - chemistry</topic><topic>Glucuronic Acid - metabolism</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Hepatocyte</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>Hexuronic Acids - chemistry</topic><topic>Hexuronic Acids - metabolism</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Male</topic><topic>Mechanical Phenomena</topic><topic>Microcapsules</topic><topic>Molecular Weight</topic><topic>Permeability</topic><topic>Polylysine - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Solubility</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lou, Ruyun</creatorcontrib><creatorcontrib>Xie, Hongguo</creatorcontrib><creatorcontrib>Zheng, Huizhen</creatorcontrib><creatorcontrib>Ren, Ying</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Guo, Xin</creatorcontrib><creatorcontrib>Song, Yizhe</creatorcontrib><creatorcontrib>Yu, Weiting</creatorcontrib><creatorcontrib>Liu, Xiudong</creatorcontrib><creatorcontrib>Ma, Xiaojun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lou, Ruyun</au><au>Xie, Hongguo</au><au>Zheng, Huizhen</au><au>Ren, Ying</au><au>Gao, Meng</au><au>Guo, Xin</au><au>Song, Yizhe</au><au>Yu, Weiting</au><au>Liu, Xiudong</au><au>Ma, Xiaojun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2016-12</date><risdate>2016</risdate><volume>93</volume><issue>Pt A</issue><spage>1133</spage><epage>1140</epage><pages>1133-1140</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte–matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27667543</pmid><doi>10.1016/j.ijbiomac.2016.09.078</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alginates - chemistry Alginates - metabolism Alginates - pharmacology Animals Asialoglycoproteins - metabolism Biological Transport Capsules Cell Survival - drug effects Chitosan - chemistry Galactose - chemistry Galactosylated chitosan Glucuronic Acid - chemistry Glucuronic Acid - metabolism Glucuronic Acid - pharmacology Hepatocyte Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - metabolism Hexuronic Acids - chemistry Hexuronic Acids - metabolism Hexuronic Acids - pharmacology Male Mechanical Phenomena Microcapsules Molecular Weight Permeability Polylysine - chemistry Rats Rats, Sprague-Dawley Solubility Water - chemistry |
| title | Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications |
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